In vitro and in vivo assessment of a bilayered degradable rapamycin-eluting stent for ureteral stricture caused by holmium: YAG laser lithotripsy

doi:10.1016/j.actbio.2023.10.009

IMR OpenIR

	In vitro and in vivo assessment of a bilayered degradable rapamycin-eluting stent for ureteral stricture caused by holmium: YAG laser lithotripsy
	Hu, Jiyuan 1,2; Wang, Zhenyu 2; Hu, Hao 3; Zhao, Jing 4; Li, Hongwei 2; Zhang, Xinyu 2; Bi, Jianbin 1; Li, Jianzhong 1,2
通讯作者	Hu, Hao(huhao@bjmu.edu.cn) ; Li, Jianzhong(174030098@qq.com)
	2023-12-01
发表期刊	ACTA BIOMATERIALIA
ISSN	1742-7061
卷号	172 页码:321-329
摘要	Ureteral stricture caused by holmium: YAG laser lithotripsy is one of the most challenging issues for urologists. Currently, evidence for rapamycin application in reducing ureterostenosis is not sufficient. This study aimed to assess the inhibition of ureteral stricture of rapamycin-eluting stents in vitro and in vivo. A bilayered drug-eluting ureteral stent consisted of drug blending with poly (lactic-co-glycolic acid) (PU/drug stent), which was over-layered by polycaprolactone (PCL) by ultrasonic atomizing spraying. Stent morphology was observed by scanning electron microscope. A kidney-ureter-bladder model was established to simulate the stents-releasing condition, and high-performance liquid chromatography was used to measure the drug release rate. The inhibitory proliferation was detected by CCK-8. The bladder of rats was injured through electro tome, and stents were implanted for 7, 14, and 28 days. The effects of drug-eluting stents was investigated by hematoxylin-eosin staining, immunofluorescence staining, realtime quantitative polymerase chain reaction and western blot. The bilayered stents could block the burst loss of the drug and maintained a sustained delivery period because of the 5.3 mu m thickness of the PCL layer. The relative growth rates of cells plotted inhibitory effect on the proliferation of human urethral scar fibroblast cells. For in vivo results of 28 days, the bilayered stent maintained structural integrity and induced less deposition of crystals, thinner and less lamina propria connective tissues were formed, and alpha-SMA and TGF-beta 1 were downregulated. Bilayered rapamycin-eluting stent is significantly effective in alleviating fibrosis in in vitro and in vivo models.
关键词	Ureteral stricture Bilayer drug-eluting Ureteral stent Rapamycin Lithotripsy
资助者	Shenyang Public Health Science and Technology Project ; Capital's Funds for Health Improvement and Research
DOI	10.1016/j.actbio.2023.10.009
收录类别	SCI
语种	英语
资助项目	Shenyang Public Health Science and Technology Project[21-172-9-10] ; Capital's Funds for Health Improvement and Research[2020-2Z-40810]
WOS研究方向	Engineering ; Materials Science
WOS类目	Engineering, Biomedical ; Materials Science, Biomaterials
WOS记录号	WOS:001154340700001
出版者	ELSEVIER SCI LTD
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/183973
专题	中国科学院金属研究所
通讯作者	Hu, Hao; Li, Jianzhong
作者单位	1.China Med Univ, Affiliated Hosp 1, Dept Urol, Shenyang 110002, Peoples R China 2.Gen Hosp Northern Theater Command, Dept Urol, Shenyang 110840, Peoples R China 3.Peking Univ, Dept Urol, Peoples Hosp, Beijing 100044, Peoples R China 4.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Hu, Jiyuan,Wang, Zhenyu,Hu, Hao,et al. In vitro and in vivo assessment of a bilayered degradable rapamycin-eluting stent for ureteral stricture caused by holmium: YAG laser lithotripsy[J]. ACTA BIOMATERIALIA,2023,172:321-329.
APA	Hu, Jiyuan.,Wang, Zhenyu.,Hu, Hao.,Zhao, Jing.,Li, Hongwei.,...&Li, Jianzhong.(2023).In vitro and in vivo assessment of a bilayered degradable rapamycin-eluting stent for ureteral stricture caused by holmium: YAG laser lithotripsy.ACTA BIOMATERIALIA,172,321-329.
MLA	Hu, Jiyuan,et al."In vitro and in vivo assessment of a bilayered degradable rapamycin-eluting stent for ureteral stricture caused by holmium: YAG laser lithotripsy".ACTA BIOMATERIALIA 172(2023):321-329.