Improving osteoinduction and osteogenesis of Ti6Al4V alloy porous scaffold by regulating the pore structure

doi:10.3389/fchem.2023.1190630

IMR OpenIR

	Improving osteoinduction and osteogenesis of Ti6Al4V alloy porous scaffold by regulating the pore structure
	Wang, Chao 1,2; Wu, Jie 1,2; Liu, Leyi 1,2; Xu, Duoling 1,2; Liu, Yuanbo 1,2; Li, Shujun 3; Hou, Wentao 3; Wang, Jian 4; Chen, Xun 1,2; Sheng, Liyuan 4; Lin, Huancai 1,2; Yu, Dongsheng 1,2
通讯作者	Sheng, Liyuan(lysheng@yeah.net) ; Lin, Huancai(linhc@mail.sysu.edu.cn) ; Yu, Dongsheng(yudsh@mail.sysu.edu.cn)
	2023-05-17
发表期刊	FRONTIERS IN CHEMISTRY
ISSN	2296-2646
卷号	11 页码:15
摘要	Titanium alloy scaffolds with a porous structure have attracted much attention in bone defect repair. However, which pore structure is more beneficial to bone defect repair is controversial. In the present research, the Ti6Al4V alloy porous scaffolds with gradient pore sizes were designed and fabricated. The microstructure characterization, tests of mechanical properties, and in vitro and in vivo experiments have been performed to systematically evaluate the effect of pore size on osteoinduction and osteogenesis. The results revealed that the contact angle with water, compressive strength, and elastic modulus of the Ti6Al4V alloy porous scaffolds decreased gradually with the increase of pore size. However, there were obvious drops when the pore size of the porous scaffold was around 600 mu m. As the pore size increased, the proliferation and integrin beta 1 of RAW 264.7 macrophages seeded on Ti6Al4V alloy porous scaffolds increased at first, reaching a maximum value at a pore size of around 600 mu m, and then decreased subsequently. The proliferation, integrin beta 1, and osteogenic gene-related expressions of Bone marrow mesenchymal stem cells (BMSCs) seeded on Ti6Al4V alloy porous scaffolds with different pore sizes all exhibited similar variations which rose with increased pore size firstly, obtaining the maximum value at pore size about 600 mu m, and then declined. The in vivo experiments confirmed the in vitro results, and the Ti6Al4V alloy porous scaffold with a pore size of 600 mu m possessed the better capability to induce new bone formation. Therefore, for the design of Ti6Al4V alloy with a regular porous scaffold, the surface morphology, porosity, strength, and elastic modulus should be considered systematically, which would determine the capability of osteoinduction and osteogenesis.
关键词	porous scaffold Ti6Al4V alloy pore structure osteogenesis bone defect repairing
资助者	National Natural Science Foundation of China ; Special Funds for the Cultivation of Guangdong College Students' Scientific and Technological Innovation
DOI	10.3389/fchem.2023.1190630
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[81974146] ; National Natural Science Foundation of China[U2241245] ; National Natural Science Foundation of China[51871220] ; Special Funds for the Cultivation of Guangdong College Students' Scientific and Technological Innovation[pdjh 2022b0012] ; Special Funds for the Cultivation of Guangdong College Students' Scientific and Technological Innovation[pdjh 2023a0004]
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:001159067000001
出版者	FRONTIERS MEDIA SA
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/184239
专题	中国科学院金属研究所
通讯作者	Sheng, Liyuan; Lin, Huancai; Yu, Dongsheng
作者单位	1.Sun Yat Sen Univ, Hosp Stomatol, Guanghua Sch Stomatol, Guangzhou, Peoples R China 2.Sun Yat Sen Univ, Guangdong Prov Key Lab Stomatol, Guangzhou, Peoples R China 3.Chinese Acad Sci, Inst Met Res, Shenyang, Peoples R China 4.Peking Univ, Shenzhen Inst, Shenzhen, Peoples R China
推荐引用方式 GB/T 7714	Wang, Chao,Wu, Jie,Liu, Leyi,et al. Improving osteoinduction and osteogenesis of Ti6Al4V alloy porous scaffold by regulating the pore structure[J]. FRONTIERS IN CHEMISTRY,2023,11:15.
APA	Wang, Chao.,Wu, Jie.,Liu, Leyi.,Xu, Duoling.,Liu, Yuanbo.,...&Yu, Dongsheng.(2023).Improving osteoinduction and osteogenesis of Ti6Al4V alloy porous scaffold by regulating the pore structure.FRONTIERS IN CHEMISTRY,11,15.
MLA	Wang, Chao,et al."Improving osteoinduction and osteogenesis of Ti6Al4V alloy porous scaffold by regulating the pore structure".FRONTIERS IN CHEMISTRY 11(2023):15.