Enhancing the angiogenesis and osteogenesis of low elastic modulus Ti-Nb-Zr-Sn alloy surface through grafting growth factors-containing gelatin nanoparticles

doi:10.1016/j.surfin.2024.104136

IMR OpenIR

	Enhancing the angiogenesis and osteogenesis of low elastic modulus Ti-Nb-Zr-Sn alloy surface through grafting growth factors-containing gelatin nanoparticles
	Yeh, Chia-En 1; Chen, Wei-Cheng 2,3; Chen, Chiang-Sang 4,5; Hou, Wen-Tao 6; Li, Shu-Jun 6; Hao, Yu-Lin 6; Sun, Ying-Sui 7,8; Huang, Her-Hsiung 1,8,9,10,11
通讯作者	Huang, Her-Hsiung(beer.huang@nycu.edu.tw)
	2024-03-01
发表期刊	SURFACES AND INTERFACES
ISSN	2468-0230
卷号	46 页码:15
摘要	The study aimed to apply a multiple surface modification for low elastic modulus Ti-24 % Nb-4 % Zr-8 % Sn (Ti2448) alloy, to enhance its biological properties for bone implant applications. We employed a combined sandblasting/acid etching/alkaline etching process to modify the Ti2448 alloy surfaces with mixed nano/ submicron/micron-scaled pits and super wettability. We prepared gelatin nanoparticles (GNPs) loaded with growth factors: vascular endothelial growth factor/platelet-derived growth factor (VEGF/PDGF) and/or bone morphogenetic protein-2 (BMP-2). The growth factors-containing GNPs were then sequentially grafted onto the surface-modified Ti2448 alloy in a layered (single layer: BMP-2 or VEGF/PDGF; double layer: inner BMP-2 and outer VEGF/PDGF) or mixed BMP-2/VEGF/PDGF manner. Surface characterizations, including morphology, roughness, surface energy, and cytotoxicity, of Ti2448 alloy were analyzed. For biological response, we investigated the tube formation ability of human umbilical vein endothelial cells (HUVECs) and the cell responses (adhesion, proliferation, and mineralization) of human bone marrow mesenchymal stem cells (hMSCs). The results demonstrated the successful preparation of GNPs with 200 similar to 300 nm particle size and their successful loading with the growth factors using natural crosslinker genipin. The growth factor-containing GNPs were effectively grafted onto the surface-modified hydrophilic Ti2448 alloy without significantly reducing its wettability (water contact angle remained below 40 degrees). The results of the study showed that the GNPs-grafted Ti2448 alloy surfaces, with the GNPs loaded with VEGF/PDGF and/or BMP-2, exhibited enhanced biological properties. The GNPs-grafted surfaces were found to be non-toxic and capable of promoting tube formation of HUVECs, particularly when single VEGF/PDGF layer or double (inner BMP-2/ outer VEGF/PDGF) layer was loaded on GNPs (enhancing the cumulative tube length approximately 1.4 times that of no GNPs-grafted surfaces). Moreover, the GNPs-grafted surfaces enhanced the adhesion-related proteins and extracellular matrix mineralization of hMSCs, particularly with the loading of single VEGF/PDGF or mixed VEGF/PDGF/BMP-2 on GNPs (enhancing alpha v beta 3 integrin patch number > 3.5 times, vinculin patch number > 2 times, and mineralization approximately 1.4 times). Based on these findings, we concluded that the proposed Ti2448 alloy surfaces, when grafted with VEGF/PDGF- and/or BMP-2-containing GNPs, have the potential for bone implant applications. The surface modifications on the Ti2448 alloy may promote angiogenesis in the early stage of bone repair (through VEGF/PDGF) and osteogenesis in the late stage (through BMP-2), thereby accelerating the overall bone healing process.
关键词	Ti-24%Nb-4%Zr-8%Sn alloy Gelatin nanoparticle Genipin Growth factor Angiogenesis Osteogenesis
资助者	National Science and Technology Council (NSTC), Taiwan ; National Science and Technology Council, Taiwan (National Taiwan University of Science and Technology)
DOI	10.1016/j.surfin.2024.104136
收录类别	SCI
语种	英语
资助项目	National Science and Technology Council (NSTC), Taiwan[108-2314-B-010-013-MY3] ; National Science and Technology Council, Taiwan (National Taiwan University of Science and Technology)[JEOL 6500F: EM0000007900] ; [NSTC 111-2731-M]
WOS研究方向	Chemistry ; Materials Science ; Physics
WOS类目	Chemistry, Physical ; Materials Science, Coatings & Films ; Physics, Applied ; Physics, Condensed Matter
WOS记录号	WOS:001208626600001
出版者	ELSEVIER
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/185651
专题	中国科学院金属研究所
通讯作者	Huang, Her-Hsiung
作者单位	1.Natl Yang Ming Chiao Tung Univ, Inst Oral Biol, Taipei 112, Taiwan 2.MacKay Med Coll, Dept Med, New Taipei 252, Taiwan 3.MacKay Mem Hosp, Dept Orthoped Surg, Div Sports Med & Surg, Taipei 104, Taiwan 4.Far Eastern Mem Hosp, Dept Orthoped Surg, New Taipei 220, Taiwan 5.Asia Eastern Univ Sci & Technol, Dept Mat & Text, New Taipei 220, Taiwan 6.Chinese Acad Sci, Inst Met Res, Shi Changxu Innovat Ctr Adv Mat, Shenyang 110016, Peoples R China 7.Taipei Med Univ, Sch Dent Technol, Taipei 110, Taiwan 8.Natl Yang Ming Chiao Tung Univ, Dept Dent, 155,Sec 2,Li Nong St, Taipei 112, Taiwan 9.China Med Univ, China Med Univ Hosp, Dept Med Res, Taichung 404, Taiwan 10.Asia Univ, Dept Bioinformat & Med Engn, Taichung 413, Taiwan 11.Kaohsiung Med Univ, Sch Dent, Kaohsiung 807, Taiwan
推荐引用方式 GB/T 7714	Yeh, Chia-En,Chen, Wei-Cheng,Chen, Chiang-Sang,et al. Enhancing the angiogenesis and osteogenesis of low elastic modulus Ti-Nb-Zr-Sn alloy surface through grafting growth factors-containing gelatin nanoparticles[J]. SURFACES AND INTERFACES,2024,46:15.
APA	Yeh, Chia-En.,Chen, Wei-Cheng.,Chen, Chiang-Sang.,Hou, Wen-Tao.,Li, Shu-Jun.,...&Huang, Her-Hsiung.(2024).Enhancing the angiogenesis and osteogenesis of low elastic modulus Ti-Nb-Zr-Sn alloy surface through grafting growth factors-containing gelatin nanoparticles.SURFACES AND INTERFACES,46,15.
MLA	Yeh, Chia-En,et al."Enhancing the angiogenesis and osteogenesis of low elastic modulus Ti-Nb-Zr-Sn alloy surface through grafting growth factors-containing gelatin nanoparticles".SURFACES AND INTERFACES 46(2024):15.