Enhancing osteogenesis and angiogenesis functions for Ti-24Nb-4Zr-8Sn scaffolds with methacrylated gelatin and deferoxamine

doi:10.3389/fbioe.2024.1372636

IMR OpenIR

	Enhancing osteogenesis and angiogenesis functions for Ti-24Nb-4Zr-8Sn scaffolds with methacrylated gelatin and deferoxamine
	Xu, Qian 1,2; Bai, Yun 2,3; Li, Shujun 2,3; Hou, Wentao 2,3; Hao, Yulin 2,3; Yang, Rui 2,3; Li, Xiaowu 1; Zhang, Xing 2,3
通讯作者	Li, Xiaowu(xwli@mail.neu.edu.cn) ; Zhang, Xing(xingzhang@imr.ac.cn)
	2024-04-19
发表期刊	FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
ISSN	2296-4185
卷号	12 页码:11
摘要	Repair of large bone defects remains challenge for orthopedic clinical treatment. Porous titanium alloys have been widely fabricated by the additive manufacturing, which possess the elastic modulus close to that of human cortical bone, good osteoconductivity and osteointegration. However, insufficient bone regeneration and vascularization inside the porous titanium scaffolds severely limit their capability for repair of large-size bone defects. Therefore, it is crucially important to improve the osteogenic function and vascularization of the titanium scaffolds. Herein, methacrylated gelatin (GelMA) were incorporated with the porous Ti-24Nb-4Zr-8Sn (Ti2448) scaffolds prepared by the electron beam melting (EBM) method (Ti2448-GelMA). Besides, the deferoxamine (DFO) as an angiogenic agent was doped into the Ti2448-GelMA scaffold (Ti2448-GelMA/DFO), in order to promote vascularization. The results indicate that GelMA can fully infiltrate into the pores of Ti2448 scaffolds with porous cross-linked network (average pore size: 120.2 +/- 25.1 mu m). Ti2448-GelMA scaffolds facilitated the differentiation of MC3T3-E1 cells by promoting the ALP expression and mineralization, with the amount of calcium contents similar to 2.5 times at day 14, compared with the Ti2448 scaffolds. Impressively, the number of vascular meshes for the Ti2448-GelMA/DFO group (similar to 7.2/mm2) was significantly higher than the control group (similar to 5.3/mm2) after cultivation for 9 h, demonstrating the excellent angiogenesis ability. The Ti2448-GelMA/DFO scaffolds also exhibited sustained release of DFO, with a cumulative release of 82.3% after 28 days. Therefore, Ti2448-GelMA/DFO scaffolds likely provide a new strategy to improve the osteogenesis and angiogenesis for repair of large bone defects.
关键词	Ti2448 GelMA bone scaffolds deferoxamine angiogenesis
资助者	National Natural Science Foundation of China10.13039/501100001809
DOI	10.3389/fbioe.2024.1372636
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China10.13039/501100001809
WOS研究方向	Biotechnology & Applied Microbiology ; Engineering
WOS类目	Biotechnology & Applied Microbiology ; Engineering, Biomedical
WOS记录号	WOS:001216001300001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/185856
专题	中国科学院金属研究所
通讯作者	Li, Xiaowu; Zhang, Xing
作者单位	1.Northeastern Univ, Sch Mat Sci & Engn, Dept Mat Phys & Chem, Minist Educ,Key Lab Anisotropy & Texture Mat, Shenyang, Liaoning, Peoples R China 2.Chinese Acad Sci, Inst Met Res, Shenyang, Liaoning, Peoples R China 3.Univ Sci & Technol China, Sch Mat Sci & Engn, Hefei, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Xu, Qian,Bai, Yun,Li, Shujun,et al. Enhancing osteogenesis and angiogenesis functions for Ti-24Nb-4Zr-8Sn scaffolds with methacrylated gelatin and deferoxamine[J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,2024,12:11.
APA	Xu, Qian.,Bai, Yun.,Li, Shujun.,Hou, Wentao.,Hao, Yulin.,...&Zhang, Xing.(2024).Enhancing osteogenesis and angiogenesis functions for Ti-24Nb-4Zr-8Sn scaffolds with methacrylated gelatin and deferoxamine.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,12,11.
MLA	Xu, Qian,et al."Enhancing osteogenesis and angiogenesis functions for Ti-24Nb-4Zr-8Sn scaffolds with methacrylated gelatin and deferoxamine".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 12(2024):11.