Inhibition effect of copper-bearing metals on arterial neointimal hyperplasia via the AKT/Nrf2/ARE pathway <i>in vitro</i> and <i>in vivo</i>

doi:10.1093/rb/rbae042

IMR OpenIR

	Inhibition effect of copper-bearing metals on arterial neointimal hyperplasia via the AKT/Nrf2/ARE pathway in vitro and in vivo
	Wang, Peng 1; Xu, Xiaohe 2; Gu, Guisong 3; Guo, Qianwen 1,4; Rao, Yanzhi 1,4; Yang, Ke 3; Xi, Tong 3; Yuan, Yonghui 5; Chen, Shanshan 3; Qi, Xun 1,4
通讯作者	Yang, Ke(kyang@imr.ac.cn) ; Chen, Shanshan(sschen@imr.ac.cn) ; Qi, Xun(qixun716@hotmail.com)
	2024-01-02
发表期刊	REGENERATIVE BIOMATERIALS
ISSN	2056-3418
卷号	11 页码:15
摘要	In-stent restenosis can be caused by the activation, proliferation and migration of vascular smooth muscle cells (VSMCs), which affects long-term efficacy of interventional therapy. Copper (Cu) has been proved to accelerate the endothelialization and reduce thrombosis formation, but little is known about its inhibition effect on the excessive proliferation of VSMCs. In this study, 316L-Cu stainless steel and L605-Cu cobalt-based alloy with varying Cu content were fabricated and their effects on surface property, blood compatibility and VSMCs were studied in vitro and in vivo. CCK-8 assay and EdU assay indicated that the Cu-bearing metals had obvious inhibitory effect on proliferation of VSMCs. Blood clotting and hemolysis tests showed that the Cu-bearing metals had good blood compatibility. The inhibition effect of the Cu-bearing metals on migration of cells was detected by Transwell assay. Further studies showed that Cu-bearing metals significantly decreased the mRNA expressions of bFGF, PDGF-B, HGF, Nrf2, GCLC, GCLM, NQO1 and HO1. The phosphorylation of AKT and Nrf2 protein expressions in VSMCs were significantly decreased by Cu-bearing metals. Furthermore, it was also found that SC79 and TBHQ treatments could recover the protein expressions of phospho-AKT and Nrf2, and their downstream proteins as well. Moreover, 316L-Cu stent proved its inhibitory action on the proliferation of VSMCs in vivo. In sum, the results demonstrated that the Cu-bearing metals possessed apparent inhibitory effect on proliferation and migration of VSMCs via regulating the AKT/Nrf2/ARE pathway, showing the Cu-bearing metals as promising stent materials for long-term efficacy of implantation.
关键词	copper-bearing metal neointimal hyperplasia vascular smooth muscle cells AKT/Nrf2/ARE pathway stent
资助者	National Natural Science Foundation ; Science and Technology Program of Liaoning province ; Construction Project of Liaoning Medical Imaging and Interventional Medical Engineering Research Center
DOI	10.1093/rb/rbae042
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation[82272099] ; National Natural Science Foundation[81873918] ; National Natural Science Foundation[51901227] ; Science and Technology Program of Liaoning province[2021JH2/10300063] ; Science and Technology Program of Liaoning province[2023JH26/103000016] ; Construction Project of Liaoning Medical Imaging and Interventional Medical Engineering Research Center[18-006-9-01]
WOS研究方向	Materials Science
WOS类目	Materials Science, Biomaterials
WOS记录号	WOS:001223354400001
出版者	OXFORD UNIV PRESS
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/186190
专题	中国科学院金属研究所
通讯作者	Yang, Ke; Chen, Shanshan; Qi, Xun
作者单位	1.China Med Univ, Hosp 1, Dept Intervent Therapy, Shenyang 110001, Peoples R China 2.China Med Univ, Shengjing Hosp, Dept Ophthalmol, Shenyang 110004, Peoples R China 3.Chinese Acad Sci, Inst Met Res, Shi Changxu Innovat Ctr Adv Mat, Shenyang 110016, Peoples R China 4.China Med Univ, Hosp 1, Dept Radiol, Key Lab Diagnost Imaging & Intervent Radiol Liaoni, Shenyang 110001, Peoples R China 5.China Med Univ, Liaoning Canc Hosp & Inst, Canc Hosp, Clin Res Ctr Malignant Tumor Liaoning Prov, Shenyang 110042, Peoples R China
推荐引用方式 GB/T 7714	Wang, Peng,Xu, Xiaohe,Gu, Guisong,et al. Inhibition effect of copper-bearing metals on arterial neointimal hyperplasia via the AKT/Nrf2/ARE pathway in vitro and in vivo[J]. REGENERATIVE BIOMATERIALS,2024,11:15.
APA	Wang, Peng.,Xu, Xiaohe.,Gu, Guisong.,Guo, Qianwen.,Rao, Yanzhi.,...&Qi, Xun.(2024).Inhibition effect of copper-bearing metals on arterial neointimal hyperplasia via the AKT/Nrf2/ARE pathway in vitro and in vivo.REGENERATIVE BIOMATERIALS,11,15.
MLA	Wang, Peng,et al."Inhibition effect of copper-bearing metals on arterial neointimal hyperplasia via the AKT/Nrf2/ARE pathway in vitro and in vivo".REGENERATIVE BIOMATERIALS 11(2024):15.