SOX9 functionalized scaffolds as a barrier to against cartilage fibrosis

doi:10.1016/j.colsurfb.2024.114011

IMR OpenIR

	SOX9 functionalized scaffolds as a barrier to against cartilage fibrosis
	Pan, Peng; Yu, Xinding; Chen, Tiantian; Liu, Wentao
通讯作者	Liu, Wentao(wtliu@imr.ac.cn)
	2024-09-01
发表期刊	COLLOIDS AND SURFACES B-BIOINTERFACES
ISSN	0927-7765
卷号	241 页码:12
摘要	Hyaline cartilage regeneration will bring evangel to millions of people suffered from cartilage diseases. However, uncontrollable cartilage fibrosis and matrix mineralization are the primary causes of cartilage regeneration failure in many tissue engineering scaffolds. This study presents a new attempt to avoid endochondral ossification or fibrosis in cartilage regeneration therapy by establishing biochemical regulatory area. Here, SOX9 expression plasmids are assembled in cellulose gels by chitosan gene vectors to fabricate SOX9+ functionalized scaffolds. RT-qPCR, western blot and biochemical analysis all show that the SOX9 reinforcement strategy can enhance chondrogenic specific proteins expression and promote GAG production. Notably, the interference from SOX9 has resisted osteogenic inducing significantly, showing an inhibition of COL1, OPN and OC production, and the inhibition efficiency was about 58.4 %, 22.8 % and 76.9 % respectively. In vivo study, implantation of these scaffolds with BMSCs can induce chondrogenic differentiation and resist endochondral ossification effectively. Moreover, specific SOX9+ functionalized area of the gel exhibited the resistance to matrix mineralization, indicating the special biochemical functional area for cartilage regeneration. These results indicate that this strategy is effective for promoting the hyaline cartilage regeneration and avoiding cartilage fibrosis, which provides a new insight to the future development of cartilage regeneration scaffolds.
关键词	Hyaline cartilage regeneration Nonphysical barrier SOX9+functionalized scaffolds Cartilage fibrosis
资助者	Applied Basic Research Programs of Liaoning Province ; Interdisciplinary Convergence Foundation of Medical Engineering of Natural Science Foundation of Liaoning Province ; Shenyang National Laboratory for Materials Science ; Institute of Metal Research, Chinese Academy of Sciences ; Joint Open Fund of State Key Laboratory of Robotics ; China Medical University (Shenyang, China)
DOI	10.1016/j.colsurfb.2024.114011
收录类别	SCI
语种	英语
资助项目	Applied Basic Research Programs of Liaoning Province[2022JH2/101300104] ; Interdisciplinary Convergence Foundation of Medical Engineering of Natural Science Foundation of Liaoning Province[2022-YGJC-02] ; Shenyang National Laboratory for Materials Science ; Institute of Metal Research, Chinese Academy of Sciences[E01SL607] ; Joint Open Fund of State Key Laboratory of Robotics[2022-KF-12-01] ; China Medical University (Shenyang, China)
WOS研究方向	Biophysics ; Chemistry ; Materials Science
WOS类目	Biophysics ; Chemistry, Physical ; Materials Science, Biomaterials
WOS记录号	WOS:001250588400001
出版者	ELSEVIER
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/187241
专题	中国科学院金属研究所
通讯作者	Liu, Wentao
作者单位	Chinese Acad Sci, Inst Met Res, Shenyang Natl Lab Mat Sci, Shenyang 110016, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Pan, Peng,Yu, Xinding,Chen, Tiantian,et al. SOX9 functionalized scaffolds as a barrier to against cartilage fibrosis[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2024,241:12.
APA	Pan, Peng,Yu, Xinding,Chen, Tiantian,&Liu, Wentao.(2024).SOX9 functionalized scaffolds as a barrier to against cartilage fibrosis.COLLOIDS AND SURFACES B-BIOINTERFACES,241,12.
MLA	Pan, Peng,et al."SOX9 functionalized scaffolds as a barrier to against cartilage fibrosis".COLLOIDS AND SURFACES B-BIOINTERFACES 241(2024):12.