Osteoinduction and Osteoconduction Evaluation of Biodegradable Magnesium Alloy Scaffolds in Repairing Large Segmental Defects in Long Bones of Rabbit Models

doi:10.1021/acsomega.4c07635

IMR OpenIR

	Osteoinduction and Osteoconduction Evaluation of Biodegradable Magnesium Alloy Scaffolds in Repairing Large Segmental Defects in Long Bones of Rabbit Models
	Liu, Shujun 1; Gao, Ming 2; Liu, Na 1; Li, Ruixin 1; Zhang, Zhiyong 1; Yao, Yuan 1; Wang, Weiming 1; Tan, Lili 2; Zhang, Nan 1,3
通讯作者	Zhang, Nan(zhn1979-08@163.com)
	2024-11-08
发表期刊	ACS OMEGA
ISSN	2470-1343
卷号	9 期号:46 页码:46419-46428
摘要	Magnesium (Mg) alloy scaffolds demonstrate promising potential for clinical applications in the repair of segmental bone defects. However, the specific mechanisms of osteoconduction and osteoinduction facilitated by these scaffolds themselves during the bone reconstruction process remain inadequately defined. This investigation systematically assesses the properties of MAO-coated Mg base implants both in vitro and in vivo. Furthermore, it elucidates the correlation between scaffold characteristics and bone regeneration in the repair of extensive long-bone defects, measuring up to 20 mm, without the use of additional bone graft materials. Electrochemical measurements and immersion tests conducted in vitro indicate that the MAO coating substantially enhances the corrosion resistance of the underlying Mg alloy. Meanwhile, the application of MAO coatings has been shown to significantly improve cytocompatibility, cellular adhesion, and osteogenic differentiation, as evidenced by the CCK-8 assays, ALP activity measurements, Western blot, and RT-qPCR in vitro. At 24 weeks postimplantation with the MAO-coated Mg alloy scaffold, the large segmental defects were effectively repaired concerning both integrity and continuity. The Micro-CT gradual replacement of old bone with new bone on the implant surface was observed by X-ray and Micro-CT. Meanwhile, the histological results indicated that the MAO-coated Mg alloy scaffold maintained a robust osteogenic response. In summary, the MAO-coated Mg alloy scaffold independently exhibits effective osteoconduction and osteoinduction, playing a significant role in bone repair function without the need for additional bone graft materials.
资助者	Affiliated Xinhua Hospital of Dalian University ; Key Discipline Project of Affiliated Xinhua Hospital of Dalian University ; Interdisciplinary Project of Dalian University ; Basic Applied Research Program of Liaoning Province of China
DOI	10.1021/acsomega.4c07635
收录类别	SCI
语种	英语
资助项目	Affiliated Xinhua Hospital of Dalian University[2022003] ; Key Discipline Project of Affiliated Xinhua Hospital of Dalian University[DLUXK-2023-YB-008] ; Interdisciplinary Project of Dalian University[2022020347-JH2/1013] ; Basic Applied Research Program of Liaoning Province of China
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:001351702800001
出版者	AMER CHEMICAL SOC
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/191195
专题	中国科学院金属研究所
通讯作者	Zhang, Nan
作者单位	1.Dalian Univ, Affiliated Xinhua Hosp, Dept Orthopaed, Dalian 116001, Peoples R China 2.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Peoples R China 3.156 Wansui St, Dalian, Liiaoning, Peoples R China
推荐引用方式 GB/T 7714	Liu, Shujun,Gao, Ming,Liu, Na,et al. Osteoinduction and Osteoconduction Evaluation of Biodegradable Magnesium Alloy Scaffolds in Repairing Large Segmental Defects in Long Bones of Rabbit Models[J]. ACS OMEGA,2024,9(46):46419-46428.
APA	Liu, Shujun.,Gao, Ming.,Liu, Na.,Li, Ruixin.,Zhang, Zhiyong.,...&Zhang, Nan.(2024).Osteoinduction and Osteoconduction Evaluation of Biodegradable Magnesium Alloy Scaffolds in Repairing Large Segmental Defects in Long Bones of Rabbit Models.ACS OMEGA,9(46),46419-46428.
MLA	Liu, Shujun,et al."Osteoinduction and Osteoconduction Evaluation of Biodegradable Magnesium Alloy Scaffolds in Repairing Large Segmental Defects in Long Bones of Rabbit Models".ACS OMEGA 9.46(2024):46419-46428.