Antibacterial effect of 317L stainless steel contained copper in prevention of implant-related infection in vitro and in vivo

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	Antibacterial effect of 317L stainless steel contained copper in prevention of implant-related infection in vitro and in vivo
	H. W. Chai; L. Guo; X. T. Wang; Y. P. Fu; J. L. Guan; L. L. Tan; L. Ren; K. Yang
	2011
发表期刊	Journal of Materials Science-Materials in Medicine
ISSN	0957-4530
卷号	22 期号:11 页码:2525-2535
摘要	Bone and intramedullary bacterial infections are one of the most serious complications of the surgical repair of fractures. To reduce the incidence of implant-related infections, several biomaterial surface treatments with integrated antibiotics, antiseptics, or metal ions have been developed for implants. In this study, we evaluated the antibacterial activity and biocompatibility of 317L stainless steel containing 4.5% copper alloy (317L-Cu) in vitro and in vivo using an animal model. Common pathogens of implant-related infections are Staphylococcus aureus and Escherichia coli, which were injected into implant materials to study their antimicrobial potential. We compared antimicrobial potential of 317L-Cu with 317L stainless steel (317L) and titanium (Ti-6Al-4V) alloys as controls. Compared with controls, 317L-Cu materials inhibited colonization by both bacteria in vitro and in vivo. Compared with 317L and Ti-6Al-4V controls, 317L-Cu showed no significant difference in colony formation of osteoblast-like cells on metal surfaces after 72 h of incubation in vitro. Metal screws containing these materials were also made for our vivo study in a rabbit model. Tissue-implants were analyzed for infection and inflammatory changes by hematoxylin-eosin staining of implants in bone. The screw tract inflammation and infection of 317L-Cu was minimal, although some inflammatory cells gathered at acutely infected sites. In addition, after materials had been implanted for 14 days in vivo, the expression of insulin-like growth factor-1 (IGF-1) in osteoblasts around 317L-Cu screws tracts had increased compared with 317L and Ti-6Al-4V controls. Overall, 317L-Cu demonstrated strong antimicrobial activity and biocompatibility in vitro and in vivo and may be used as a biomaterial to reduce implant-related infections.
部门归属	[chai, hongwei; guo, lei; wang, xiantao; fu, yuping; guan, junlin] china med univ, dept orthoped surg, affiliated hosp 1, shenyang 110001, liaoning, peoples r china. [tan, lili; ren, ling; yang, ke] chinese acad sci, inst met res, shenyang 110016, liaoning, peoples r china.;guo, l (reprint author), china med univ, dept orthoped surg, affiliated hosp 1, shenyang 110001, liaoning, peoples r china;g572@sina.com
关键词	Resistant Staphylococcus-aureus Central Venous Catheters Antimicrobial Activity Surfaces Survival Alloys Bone Osteomyelitis Pathogens Efficacy
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WOS记录号	WOS:000297621900014
引用统计	被引频次：116[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/30245
专题	中国科学院金属研究所
推荐引用方式 GB/T 7714	H. W. Chai,L. Guo,X. T. Wang,et al. Antibacterial effect of 317L stainless steel contained copper in prevention of implant-related infection in vitro and in vivo[J]. Journal of Materials Science-Materials in Medicine,2011,22(11):2525-2535.
APA	H. W. Chai.,L. Guo.,X. T. Wang.,Y. P. Fu.,J. L. Guan.,...&K. Yang.(2011).Antibacterial effect of 317L stainless steel contained copper in prevention of implant-related infection in vitro and in vivo.Journal of Materials Science-Materials in Medicine,22(11),2525-2535.
MLA	H. W. Chai,et al."Antibacterial effect of 317L stainless steel contained copper in prevention of implant-related infection in vitro and in vivo".Journal of Materials Science-Materials in Medicine 22.11(2011):2525-2535.

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