Pathway of programmed cell death in HeLa cells induced by polymeric anti-cancer drugs

IMR OpenIR

	Pathway of programmed cell death in HeLa cells induced by polymeric anti-cancer drugs
	Y. Q. Guan; Z. B. Li; J. M. Chen; H. M. Tao; W. W. Wang; Z. Zheng; L. Li; J. M. Liu
	2011
发表期刊	Biomaterials
ISSN	0142-9612
卷号	32 期号:14 页码:3637-3646
摘要	Synthesis of anticancer polymeric materials plus their biological applications is one of the most charming and active research areas in biological functional materials. However, the predominant mechanisms for controlling cancer cell viability are not yet clear. In this work, cell culture polymeric materials co-immobilized with death signal proteins interferon-gamma (IFN-gamma)/tumor necrosis factor-alpha (TNF-alpha) on the surface were prepared by photochemical method to develop an anticancer polymeric drug model. Various characterizations on the microstructures and compositions, including the Fourier transform infrared spectroscopy, UV absorption spectroscopy, fluorescence measurement, atomic force microscopy, and electron spectroscopy for chemical analysis, were performed. For addressing the biological applications, we investigated systematically the death pathways of HeLa cells attached onto the drug model by means of a series of cell-biology techniques. It was demonstrated that the IFN-gamma plus TNF-alpha co-immobilized on the polymeric material surface exhibited more notable inhibitive effects than the free IFN-gamma plus TNF-alpha, and the induced HeLa cells were mainly along apoptosis-like PCD with the translocation of EndoG from the cytoplasm to the nucleus. These findings indicate that the polymeric drugs with the co-immobilized IFN-gamma plus TNF-alpha may offer significant potentials for therapeutic manipulation of human cervical cancer. (C) 2011 Elsevier Ltd. All rights reserved.
部门归属	[liu, jun-ming] nanjing univ, solid state microstruct lab, nanjing 210093, peoples r china. [guan, yan-qing; li, zhibin; chen, jiamei; tao, huimin; wang, wenwen; zheng, zhe; li, ling] s china normal univ, sch life sci, guangzhou 510613, guangdong, peoples r china. [liu, jun-ming] s china normal univ, sch phys, guangzhou 510613, guangdong, peoples r china. [liu, jun-ming] chinese acad sci, int ctr mat phys, shenyang 110016, peoples r china.;liu, jm (reprint author), nanjing univ, solid state microstruct lab, hankou rd 22, nanjing 210093, peoples r china;liujm@nju.edu.cn
关键词	Co-immobilized Ifn-gamma Plus Tnf-alpha Polystyrene Material Hela Endog Tumor-necrosis-factor Nf-kappa-b Ifn-gamma Interferon-gamma Endonuclease-g Factor-alpha Apoptosis Activation Micelles P53
URL	查看原文
WOS记录号	WOS:000289020600011
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/30364
专题	中国科学院金属研究所
推荐引用方式 GB/T 7714	Y. Q. Guan,Z. B. Li,J. M. Chen,et al. Pathway of programmed cell death in HeLa cells induced by polymeric anti-cancer drugs[J]. Biomaterials,2011,32(14):3637-3646.
APA	Y. Q. Guan.,Z. B. Li.,J. M. Chen.,H. M. Tao.,W. W. Wang.,...&J. M. Liu.(2011).Pathway of programmed cell death in HeLa cells induced by polymeric anti-cancer drugs.Biomaterials,32(14),3637-3646.
MLA	Y. Q. Guan,et al."Pathway of programmed cell death in HeLa cells induced by polymeric anti-cancer drugs".Biomaterials 32.14(2011):3637-3646.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Pathway of programme（2601KB）			开放获取	--