Death signal transduction induced by co-immobilized TNF-alpha plus IFN-gamma and the development of polymeric anti-cancer drugs

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	Death signal transduction induced by co-immobilized TNF-alpha plus IFN-gamma and the development of polymeric anti-cancer drugs
	Y. Q. Guan; Z. B. Li; J. M. Liu
	2010
发表期刊	Biomaterials
ISSN	0142-9612
卷号	31 期号:34 页码:9074-9085
摘要	Based on our earlier work on the apoptosis in HeLa cells induced by TNF-alpha plus IFN-gamma, we investigate how the co-immobilized TNF-alpha plus IFN-gamma promotes the signal transduction of HeLa cells. It is found that the free TNF-alpha plus IFN-gamma has much stronger capability than the co-immobilized TNF-alpha plus IFN-gamma in binding with apoptosis signaling receptors TNFR1, which allows an argument that the co-immobilized TNF-alpha plus IFN-gamma can modulate the death pathway of HeLa cells. Subsequently, we determine the cell membrane surface receptor with which the co-immobilized TNF-alpha plus IFN-gamma binds, and probe the expression of death receptor which induces the apoptosis pathway upstream protein FADD and TRADD. Our results reveal that the death signal transduction, induced by the co-immobilized TNF-alpha plus IFN-gamma, is mainly realized via the IFN-gamma signaling pathway rather than the TNF-alpha one. In addition, the transcription of STAT1 plus its Serine 727 and Tyrosine 701 phosphorylation is not the pre-requisite for inducing the cell death signal transduction. It is thus suggested that the co-immobilized TNF-alpha plus IFN-gamma promotes the activation of some unknown key markers in response to IFN-gamma, and the binding of the co-immobilized TNF-alpha plus IFN-gamma with some other TNF-alpha receptors results in enhanced programmed cell death in HeLa cells. (C) 2010 Elsevier Ltd. All rights reserved.
部门归属	[liu, jun-ming] nanjing univ, solid state microstruct lab, nanjing 210093, peoples r china. [liu, jun-ming] chinese acad sci, int ctr mat phys, shenyang 110016, peoples r china. [liu, jun-ming] s china normal univ, sch phys, guangzhou 510613, guangdong, peoples r china. [guan, yan-qing; li, zhibin] s china normal univ, moe key lab laser life sci, guangzhou 510613, guangdong, peoples r china. [guan, yan-qing; li, zhibin] s china normal univ, sch life sci, guangzhou 510613, guangdong, peoples r china.;liu, jm (reprint author), nanjing univ, solid state microstruct lab, nanjing 210093, peoples r china;liujm@nju.edu.cn
关键词	Hela Co-immobilized Tnf-alpha Plus Ifn-gamma Tnfr1 Ifnr2 Stat1 Tyrosine 701 Phosphorylation Tumor-necrosis-factor Nf-kappa-b Mesenchymal Stem-cells Interferon-gamma Transcription Factors Surface Modification Induced Apoptosis Factor Receptor Growth-factor Activation
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WOS记录号	WOS:000283814600029
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/31123
专题	中国科学院金属研究所
推荐引用方式 GB/T 7714	Y. Q. Guan,Z. B. Li,J. M. Liu. Death signal transduction induced by co-immobilized TNF-alpha plus IFN-gamma and the development of polymeric anti-cancer drugs[J]. Biomaterials,2010,31(34):9074-9085.
APA	Y. Q. Guan,Z. B. Li,&J. M. Liu.(2010).Death signal transduction induced by co-immobilized TNF-alpha plus IFN-gamma and the development of polymeric anti-cancer drugs.Biomaterials,31(34),9074-9085.
MLA	Y. Q. Guan,et al."Death signal transduction induced by co-immobilized TNF-alpha plus IFN-gamma and the development of polymeric anti-cancer drugs".Biomaterials 31.34(2010):9074-9085.