Synthesis of a Kind of Temperature-responsive Cell Culture Surface for Corneal Sheet

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	Synthesis of a Kind of Temperature-responsive Cell Culture Surface for Corneal Sheet
	Y. Q. Guan; Z. B. Li; X. Wang; X. L. Ni; A. N. Yang; J. M. Liu
	2010
发表期刊	Journal of Materials Science & Technology
ISSN	1005-0302
卷号	26 期号:12 页码:1119-1126
摘要	In this study, acrylic acid (AA) and 4-azidoaniline were used to modify poly (N-isopropylacrylamide) (NI-PAAm) in order to fabricate temperature-responsive surface for corneal epithelia cell adhesion and detachment. First, NIPAAm was copolymerized with acrylic acid. Then, the copolymer was coupled with azidoaniline to synthesize AzPhPIA, derivative of p(NIPAAm-co-AA), which possesses both thermo- and photo-sensitivities. Second, the synthesized copolymer was characterized by high performance liquid chromatography (HPLC), Fourier transform infrared (FTIR) and a CHN analyzer. The thermo-sensitivity was characterized by temperature reducing experiment, contact angle measurement and low critical solution temperature (LCST) testing. Third, the derivatized copolymer was immobilized by photolithography on a polystyrene plate, and then the surface characterization of AzPhPIA-coated polystyrene plate (PSt) was measured by electron spectroscopy for chemical analysis (ESCA). The thermo-sensitivity and cytocompatibility of the AzPhPIA-coated PSt were investigated by corneal epithelial cells culture. The results revealed that the AzPhPIA-coated PSt exhibited good cytocompatibility and cell detachability when temperature decreased.
部门归属	[liu, junming] s china normal univ, sch phys, guangzhou 510631, guangdong, peoples r china. [guan, yanqing; li, zhibin; wang, xin; ni, xiaoli; yang, aini] s china normal univ, sch life sci, guangzhou 510631, guangdong, peoples r china. [liu, junming] nanjing univ, lab solid state microstruct, nanjing 210093, peoples r china. [liu, junming] chinese acad sci, int ctr mat phys, shenyang 110016, peoples r china.;liu, jm (reprint author), s china normal univ, sch phys, guangzhou 510631, guangdong, peoples r china;liujm@nju.edu.cn
关键词	N-isopropylacrylamide Low Critical Solution Temperature Tissue Engineering Corneal Epithelial Cells N-isopropylacrylamide Stem-cells Hydrogels Grafts
URL	查看原文
WOS记录号	WOS:000286153900011
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/31124
专题	中国科学院金属研究所
推荐引用方式 GB/T 7714	Y. Q. Guan,Z. B. Li,X. Wang,et al. Synthesis of a Kind of Temperature-responsive Cell Culture Surface for Corneal Sheet[J]. Journal of Materials Science & Technology,2010,26(12):1119-1126.
APA	Y. Q. Guan,Z. B. Li,X. Wang,X. L. Ni,A. N. Yang,&J. M. Liu.(2010).Synthesis of a Kind of Temperature-responsive Cell Culture Surface for Corneal Sheet.Journal of Materials Science & Technology,26(12),1119-1126.
MLA	Y. Q. Guan,et al."Synthesis of a Kind of Temperature-responsive Cell Culture Surface for Corneal Sheet".Journal of Materials Science & Technology 26.12(2010):1119-1126.