Effects of Different Dental Alloys on Cytotoxic and Apoptosis Related Genes Expression in L929 Cells

IMR OpenIR

	Effects of Different Dental Alloys on Cytotoxic and Apoptosis Related Genes Expression in L929 Cells
	X. Y. Jia; Q. A. Wang; H. Meng; H. Sun; D. S. Zhan
	2010
发表期刊	Journal of Hard Tissue Biology
ISSN	1341-7649
卷号	19 期号:2 页码:95-99
摘要	Cytotoxicity is one of the most important problems in medical metal materials. Effect of different dental alloys on cytotoxic of L929 cells was evaluated by in vitro cytotoxicity-test (MTT method). Metal ion concentration was one of the factors leading to occurrence of cell toxicity. Thus metal ion concentration in cell culture fluid at different intervals of immersion periods were detected by inductively coupled plasma mass spectrometry (ICP-MS). For different medical metals, RT-PCR method was also used to analysis mRNA expression of L929 cells'caspase-3, caspase-8 and caspase-9. The results indicated that MTT test found little optical density (OD) value difference for different alloys at different time points. The cytotoxicity of Cu alloy group was Grade 4 and those of other groups were all Grade 0 after 48 hours culture. The mRNA levels of caspase-8 had no change in all groups. The mRNA levels of caspase-3 and caspase-9 were as follows from low to high: Cu alloy, the negative control and Au alloy, Co-Cr alloy, Ag-Pd alloy, and Ni-Cr alloy after 48 hours' culture. It is concluded that metal ions of the studied dental alloys may induce cell apoptosis by different degree through mitochondrion pathway. Apoptosis related genes expression of L929 cells was affected by ion concentration in cell culture fluid.
部门归属	[jia, xingya; wang, qiang; meng, he; sun, hao; zhan, desong] china med univ, sch stomatol, shenyang 110002, peoples r china. [wang, qiang] chinese acad sci, inst met res, shenyang 110016, peoples r china.;zhan, ds (reprint author), china med univ, sch stomatol, nan jing n st 117, shenyang 110002, peoples r china;zhandesong@126.com
关键词	Dental Alloys Icp Fibroblast Cell L929 Apoptosis Chromium Ions In-vitro Biocompatibility Cultures Cobalt
URL	查看原文
WOS记录号	WOS:000283588300003
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/31174
专题	中国科学院金属研究所
推荐引用方式 GB/T 7714	X. Y. Jia,Q. A. Wang,H. Meng,et al. Effects of Different Dental Alloys on Cytotoxic and Apoptosis Related Genes Expression in L929 Cells[J]. Journal of Hard Tissue Biology,2010,19(2):95-99.
APA	X. Y. Jia,Q. A. Wang,H. Meng,H. Sun,&D. S. Zhan.(2010).Effects of Different Dental Alloys on Cytotoxic and Apoptosis Related Genes Expression in L929 Cells.Journal of Hard Tissue Biology,19(2),95-99.
MLA	X. Y. Jia,et al."Effects of Different Dental Alloys on Cytotoxic and Apoptosis Related Genes Expression in L929 Cells".Journal of Hard Tissue Biology 19.2(2010):95-99.