In vitro and in vivo evaluations on osteogenesis and biodegradability of a ss-tricalcium phosphate coated magnesium alloy

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	In vitro and in vivo evaluations on osteogenesis and biodegradability of a ss-tricalcium phosphate coated magnesium alloy
	H. W. Chai; L. Guo; X. T. Wang; X. Y. Gao; K. Liu; Y. P. Fu; J. L. Guan; L. L. Tan; K. Yang
	2012
发表期刊	Journal of Biomedical Materials Research Part A
ISSN	1549-3296
卷号	100A 期号:2 页码:293-304
摘要	Magnesium (Mg) alloys have been demonstrated to be potential orthopedic implants due to their biodegradability in vivo. To enhance its corrosion resistance and improve its osteogenesis, beta-tricalcium phosphate (beta-TCP) was coated on a Mg alloy (Mg-3AI-1Zn) by phosphating process. In vitro, the human osteosarcoma cell line (SaOS-2) showed significantly good adherence and proliferation on the surface of the beta-TCP coated Mg alloy after 24-h incubation. The growth factor bone morphogenetic protein 2 (BMP-2) was highly expressed in SaOS-2 cultured with the beta-TCP coated Mg alloy by Western blot analysis (p < 0.05). In vivo, the newborn bone at the implant/bone interface was formed at week 1 and matured at week 4 postimplantation. Villous tissue was found at the implant/bone interface at week 12 postimplantation. The contents of phosphorus and calcium on the surface of the beta-TCP coated Mg alloy were decreased at week 4 and week 12 postimplantation, respectively. Immunohistochemical analysis of the experiment results demonstrated that the beta-TCP coated Mg alloy implants provided a high BMP-2 expression during the first 4 weeks postimplantation. Compared with the naked Mg alloy which was degraded for 33% in vivo, only 17% of the beta-TCP coated Mg alloy was degraded at week 12 postimplantation (p < 0.05). The in vitro cell tests showed that the beta-TCP coating provided the Mg alloy with a significantly better surface cytocompatibility, and in vivo results also confirmed that the beta-TCP coating exhibited greatly improved osteoconductivity and osteogenesis in the early 12 weeks postoperation period. Moreover, in vivo experiment demonstrated that the beta-TCP coating layer could slow down the degradation of the naked Mg alloy at the early stage of implantation. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.
部门归属	[chai, hongwei; guo, lei; wang, xiantao; gao, xiaoyu; liu, kui; fu, yuping; guan, junlin; yang, ke] china med univ, affiliated hosp 1, dept orthoped surg, shenyang 110001, peoples r china. [tan, lili; yang, ke] chinese acad sci, inst met res, shenyang 110016, peoples r china.;guo, l (reprint author), china med univ, affiliated hosp 1, dept orthoped surg, shenyang 110001, peoples r china.;g572@sina.com
关键词	Magnesium Alloy Ss-tricalcium Phosphate Osteogenesis Corrosion Resistance Bone Morphogenetic Protein-2 Mechanical Integrity Cell Compatibility Calcium Silicate Ca Alloys Beta-tcp Zn Alloy Hydroxyapatite Degradation Composite
URL	查看原文
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/59891
专题	中国科学院金属研究所
推荐引用方式 GB/T 7714	H. W. Chai,L. Guo,X. T. Wang,et al. In vitro and in vivo evaluations on osteogenesis and biodegradability of a ss-tricalcium phosphate coated magnesium alloy[J]. Journal of Biomedical Materials Research Part A,2012,100A(2):293-304.
APA	H. W. Chai.,L. Guo.,X. T. Wang.,X. Y. Gao.,K. Liu.,...&K. Yang.(2012).In vitro and in vivo evaluations on osteogenesis and biodegradability of a ss-tricalcium phosphate coated magnesium alloy.Journal of Biomedical Materials Research Part A,100A(2),293-304.
MLA	H. W. Chai,et al."In vitro and in vivo evaluations on osteogenesis and biodegradability of a ss-tricalcium phosphate coated magnesium alloy".Journal of Biomedical Materials Research Part A 100A.2(2012):293-304.