Cell death in He La mediated by thermoplastic polyurethane with co-immobilized IFN-gamma plus TNF-alpha

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	Cell death in He La mediated by thermoplastic polyurethane with co-immobilized IFN-gamma plus TNF-alpha
	Y. Q. Guan; Z. Zheng; Z. B. Li; J. M. Liu
	2012
发表期刊	Acta Biomaterialia
ISSN	1742-7061
卷号	8 期号:3 页码:1348-1356
摘要	In order to prohibit the toxicity of free IFN-gamma plus TNF-alpha in treating human cervical cancer HeLa cells, two kinds of thermoplastic polyurethane (polyester/polyether) biomaterials with co-immobilized IFN-gamma plus TNF-alpha on the surfaces are prepared. The programmed cell death of HeLa induced by these biomaterials is investigated. The surface modification of these biomaterials with co-immobilized IFN-gamma plus TNF-alpha is performed by the photo-immobilization method, and the surface structures are characterized by various techniques. The cell morphology, cell mortality, cell cycle arrest, and functional status of caspases, upon the treatment by these biomaterials, are characterized. The results show that the as-prepared biomaterials have high inhibition activity against the growth of HeLa cells. The HeLa cells mediated by the two kinds of biomaterials are mainly arrested in the G(1) phase, while those cells mediated directly by free IFN-gamma plus TNF-alpha are mainly arrested in the S phase. It is suggested that the programmed cell death mechanism induced by these two kinds of biomaterials is both caspase-dependent and caspase-independent. Our data provide the knowledge of microscopic surface structures and cell biology basis for synthesizing the thermoplastic polyurethane biomaterials with co-immobilized IFN-gamma plus TNF-alpha, which are promising for novel therapeutics (e.g. drug cup) design for cervical cancer patients. (C) 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
部门归属	[liu, jun-ming] nanjing univ, lab solid state microstruct, nanjing 210093, jiangsu, peoples r china. [guan, yan-qing; zheng, zhe; li, zhibin] s china normal univ, sch life sci, guangzhou 510631, guangdong, peoples r china. [liu, jun-ming] s china normal univ, inst adv mat, sch phys, guangzhou 510631, guangdong, peoples r china. [liu, jun-ming] chinese acad sci, int ctr mat phys, shenyang 110016, peoples r china.;liu, jm (reprint author), nanjing univ, lab solid state microstruct, nanjing 210093, jiangsu, peoples r china.;liujm@nju.edu.cn
关键词	Ifn-gamma Tnf-alpha Co-immobilization Thermoplastic Polyurethane (Tpu) Hela Tumor-necrosis-factor Mesenchymal Stem-cells In-vitro Interferon-gamma Growth-factor Drug-release Cancer Cells Apoptosis Differentiation Activation
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文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/59973
专题	中国科学院金属研究所
推荐引用方式 GB/T 7714	Y. Q. Guan,Z. Zheng,Z. B. Li,et al. Cell death in He La mediated by thermoplastic polyurethane with co-immobilized IFN-gamma plus TNF-alpha[J]. Acta Biomaterialia,2012,8(3):1348-1356.
APA	Y. Q. Guan,Z. Zheng,Z. B. Li,&J. M. Liu.(2012).Cell death in He La mediated by thermoplastic polyurethane with co-immobilized IFN-gamma plus TNF-alpha.Acta Biomaterialia,8(3),1348-1356.
MLA	Y. Q. Guan,et al."Cell death in He La mediated by thermoplastic polyurethane with co-immobilized IFN-gamma plus TNF-alpha".Acta Biomaterialia 8.3(2012):1348-1356.