Osteoimmunomodulatory properties of magnesium scaffolds coated with beta-tricalcium phosphate

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	Osteoimmunomodulatory properties of magnesium scaffolds coated with beta-tricalcium phosphate
	Z. T. Chen; X. L. Mao; L. L. Tan; T. Friis; C. T. Wu; R. Crawford; Y. Xiao
	2014
发表期刊	Biomaterials
ISSN	0142-9612
卷号	35 期号:30 页码:8553-8565
摘要	The osteoimmunomodulatory property of bone biomaterials is a vital property determining the in vivo fate of the implants. Endowing bone biomaterials with favorable osteoimmunomodulatory properties is of great importance in triggering desired immune response and thus supports the bone healing process. Magnesium (Mg) has been recognized as a revolutionary metal for applications in orthopedics due to it being biodegradable, biocompatible, and having osteoconductive properties. However, Mg's high rate of degradation leads to an excessive inflammatory response and this has restricted its application in bone tissue engineering. In this study, beta-tricalcium phosphate (beta-TCP) was used to coat Mg scaffolds in an effort to modulate the detrimental osteoimmunomodulatory properties of Mg scaffolds, due to the reported favorable osteoimmunomodulatory properties of beta-TCP. It was noted that macrophages switched to the M2 extreme phenotype in response to the Mg-beta-TCP scaffolds, which could be due to the inhibition of the toll like receptor (TLR) signaling pathway. VEGF and BMP2 were significantly upregulated in the macrophages exposed to Mg-beta-TCP scaffolds, indicating pro-osteogenic properties of macrophages in beta-TCP modified Mg scaffolds. This was further demonstrated by the macrophage-mediated osteogenic differentiation of bone marrow stromal cells (BMSCs). When BMSCs were stimulated by conditioned medium from macrophages cultured on Mg-beta-TCP scaffolds, osteogenic differentiation of BMSCs was significantly enhanced; whereas osteoclastogenesis was inhibited, as indicated by the downregualtion of MCSF, TRAP and inhibition of the RANKL/RANK system. These findings suggest that beta-TCP coating of Mg scaffolds can modulate the scaffold's osteoimmunomodulatory properties, shift the immune microenvironment towards one that favors osteogenesis over osteoclastogenesis. Endowing bone biomaterials with favorable osteoimmunomodulatory properties can be a highly valuable strategy for the development or modification of advanced bone biomaterials. (C) 2014 Elsevier Ltd. All rights reserved.
部门归属	[chen, zetao ; friis, thor ; crawford, ross ; xiao, yin] queensland univ technol, inst hlth & biomed innovat, brisbane, qld 4059, australia. [chen, zetao ; mao, xueli ; wu, chengtie ; crawford, ross ; xiao, yin] queensland univ technol, australia china ctr tissue engn & regenerat med, brisbane, qld 4059, australia. [mao, xueli] sun yat sen univ, hosp stomatol, guanghua sch stomatol, dept operat dent & endodont, guangzhou 510055, guangdong, peoples r china. [mao, xueli] guangdong prov key lab stomatol, guangzhou 510080, guangdong, peoples r china. [tan, lili] chinese acad sci, inst met res, shenyang 110016, peoples r china. [wu, chengtie] chinese acad sci, shanghai inst ceram, state key lab high performance ceram & superfine, shanghai 200050, peoples r china. ; mao, xl (reprint author), sun yat sen univ, hosp stomatol, guanghua sch stomatol, dept operat dent & endodont, 56 ling yuan rd west, guangzhou 510055, guangdong, peoples r china. ; maoxuel@mail.sysu.edu.cn ; yin.xiao@qut.edu.au
关键词	Macrophage Bone Substitute Osteoimmunomodulatory Property Beta-tcp Magnesium Osteogenesis Bone Morphogenetic Protein-2 Mesenchymal Stem-cells Endothelial Growth-factor Tgf-beta In-vitro Macrophage Activation Differentiation Osteogenesis Angiogenesis Responses
URL	查看原文
语种	英语
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/73200
专题	中国科学院金属研究所
推荐引用方式 GB/T 7714	Z. T. Chen,X. L. Mao,L. L. Tan,et al. Osteoimmunomodulatory properties of magnesium scaffolds coated with beta-tricalcium phosphate[J]. Biomaterials,2014,35(30):8553-8565.
APA	Z. T. Chen.,X. L. Mao.,L. L. Tan.,T. Friis.,C. T. Wu.,...&Y. Xiao.(2014).Osteoimmunomodulatory properties of magnesium scaffolds coated with beta-tricalcium phosphate.Biomaterials,35(30),8553-8565.
MLA	Z. T. Chen,et al."Osteoimmunomodulatory properties of magnesium scaffolds coated with beta-tricalcium phosphate".Biomaterials 35.30(2014):8553-8565.