Real time, in situ observation of the photocatalytic inactivation of Saccharomyces cerevisiae cells

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	Real time, in situ observation of the photocatalytic inactivation of Saccharomyces cerevisiae cells
	J. T.; Wang Zhang, X. X.; Li, Q.; Shang, J. K.
	2015
发表期刊	Materials Science & Engineering C-Materials for Biological Applications
ISSN	0928-4931
卷号	49 页码:75-83
摘要	An in situ microscopy technique was developed to observe in real time the photocatalytic inactivation process of Saccharomyces cerevisiae (S. cerevisiae) cells by palladium-modified nitrogen-doped titanium oxide (TiON/PdO) under visible light illumination. The technique was based on building a photocatalytic micro-reactor on the sample stage of a fluorescence/phase contrast microscopy capable of simultaneously providing the optical excitation to activate the photocatalyst in the micro-reactor and the illumination to acquire phase contrast images of the cells undergoing the photocatalytic inactivation process. Using TiON/PdO as an example, the technique revealed for the first time the vacuolar activities inside S. cerevisiae cells subjected to a visible light photocatalytic inactivation. The vacuoles responded to the photocatalytic attack by the first expansion of the vacuolar volume and then contraction, before the vacuole disappeared and the cell structure collapsed. Consistent with the aggregate behavior observed from the cell culture experiments, the transition in the vacuolar volume provided clear evidence that photocatalytic disinfection of S. cerevisiae cells started with an initiation period in which cells struggled to offset the photocatalytic damage and moved rapidly after the photocatalytic damage overwhelmed the defense mechanisms of the cells against oxidative attack. (C) 2014 Elsevier B.V. All rights reserved.
部门归属	[zhang, jingtao] zhengzhou univ flight ind, sch food & bioengn, zhengzhou 450002, peoples r china. [zhang, jingtao ; wang, xiaoxin ; li, qi ; shang, jian ku] chinese acad sci, inst met res, environm funct mat div, shenyang natl lab mat sci, shenyang 110016, peoples r china. [shang, jian ku] univ illinois, dept mat sci & engn, urbana, il 61801 usa. ; li, q (reprint author), 72 wenhua rd, shenyang 110016, liaoning provin, peoples r china. ; qili@imr.ac.cn
关键词	Photocatalytic Inactivation Saccharomyces Cerevisiae Cells Tion/pdo Real Time Analysis, In Situ Observation Phase Contrast Microscopy Fluorescence Staining Doped Titanium-oxide Visible-light Bactericidal Activity Tio2 Photocatalyst Water Disinfection Dioxide Nitrogen Degradation Irradiation Nanocomposites
URL	查看原文
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/74021
专题	中国科学院金属研究所
推荐引用方式 GB/T 7714	J. T.,Wang Zhang, X. X.,Li, Q.,et al. Real time, in situ observation of the photocatalytic inactivation of Saccharomyces cerevisiae cells[J]. Materials Science & Engineering C-Materials for Biological Applications,2015,49:75-83.
APA	J. T.,Wang Zhang, X. X.,Li, Q.,&Shang, J. K..(2015).Real time, in situ observation of the photocatalytic inactivation of Saccharomyces cerevisiae cells.Materials Science & Engineering C-Materials for Biological Applications,49,75-83.
MLA	J. T.,et al."Real time, in situ observation of the photocatalytic inactivation of Saccharomyces cerevisiae cells".Materials Science & Engineering C-Materials for Biological Applications 49(2015):75-83.