Anti-biofilm formation of a novel stainless steel against Staphylococcus aureus

IMR OpenIR

	Anti-biofilm formation of a novel stainless steel against Staphylococcus aureus
	Nan, Li; Yang, Ke; Ren, Guogang; g.g.ren@herts.ac.uk
	2015
发表期刊	MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
ISSN	0928-4931
卷号	51 页码:356-361
摘要	Staphylococcus aureus (S. aureus) is a bacterium frequently found proliferating on metal surfaces such as stainless steels used in healthcare and food processing facilities. Past research has shown that a novel Cu-bearing 304 type stainless steel (304CuSS) exhibits excellent antibacterial ability (i.e. against S. aureus) in a short time period (24 h.). This work was dedicated to investigate the 304CuSS's inhibition ability towards the S. aureus biofilm formation for an extended period of 7 days after incubation. It was found that the antibacterial rate of the 304CuSS against sessile bacterial cells reached over 99.9% in comparison with the 304SS. The thickness and sizes of the biofilms on the 304SS surfaces increased markedly with period of contact, and thus expected higher risk of bio-contamination, indicated by the changes of surface free energy between biofilm and the steel surfaces. The results demonstrated that the 304CuSS exhibited strong inhibition on the growth and adherence of the biofilms. The surface free energy of the 304CuSS after contact with sessile bacterial cells was much lower than that of the 304SS towards the same culture times. The continuously dissolved Cu2+ ions well demonstrated the dissolution ability of Cu-rich precipitates after exposure to S. aureus solution, from 3.1 ppm (2 days) to 4.5 ppm (7 days). For this to occur, a hypothesis mechanism might be established for 304CuSS in which the Cu2+ ions were released from Cu-rich phases that bond with extracellular polymeric substances (EPS) of the microorganisms. And these inhibited the activities of cell protein/enzymes and effectively prevented planktonic bacterial cells attaching to the 304CuSS metal surface. (C) 2015 The Authors. Published by Elsevier B.V.
部门归属	[nan, li ; yang, ke] chinese acad sci, inst met res, shenyang 110016, peoples r china ; [ren, guogang] univ hertfordshire, hatfield al10 9ab, herts, england
关键词	Cu-bearing Stainless Steel S. Aureus Antibacterial Biofilm
资助者	National Natural Science Foundation of China [51101154, 51371168]; National Basic Research Program [2012CB619101]; Shenyang National Lab for Materials Science; UK Royal Academy of Engineering (RAEng) [1213RECI052]
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/74637
专题	中国科学院金属研究所
通讯作者	g.g.ren@herts.ac.uk
推荐引用方式 GB/T 7714	Nan, Li,Yang, Ke,Ren, Guogang,et al. Anti-biofilm formation of a novel stainless steel against Staphylococcus aureus[J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,2015,51:356-361.
APA	Nan, Li,Yang, Ke,Ren, Guogang,&g.g.ren@herts.ac.uk.(2015).Anti-biofilm formation of a novel stainless steel against Staphylococcus aureus.MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,51,356-361.
MLA	Nan, Li,et al."Anti-biofilm formation of a novel stainless steel against Staphylococcus aureus".MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 51(2015):356-361.