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Enhanced Performance of Nanocrystalline ZnO DNA Biosensor via Introducing Electrochemical Covalent Biolinkers
Wang, Chun; Huang, Nan; Zhuang, Hao; Jiang, Xin; xjiang@imr.ac.cn
2015
Source PublicationACS APPLIED MATERIALS & INTERFACES
ISSN1944-8244
Volume7Issue:14Pages:7605-7612
AbstractZinc oxide (ZnO) is considered to be one of the most promising candidates for the third-generation DNA biosensor because of its good chemical stability, wonderful biocompatibility, easy surface modification, and numerous kinds of nanostructures. In this work, we report a new and simple method to modify ZnO surface for the immobilization of oligonucleotides by electrochemical covalent grafting of diazonium salts. The atomic force microscope, X-ray photoelectron spectroscopy, surface contact angle system, and electrochemical workstation were employed to characterize the functionalization process. Fluorescence results show that this kind of DNA biosensor from covalently linking strategy has an enhanced performance compared to that based on an electrostatic adsorption route. The functionalized ZnO biosensor has the capability to distinguish four-base mismatched, one-base mismatched, and complementary DNA sequences. Moreover, a linear relationship has been observed between the fluorescence intensity and the concentration of the complementary DNA in the solution within the range from 10(-6) to 10(-9) M, offering us a possibility in the qualitative determination of the level of target DNA.
description.department[wang, chun ; huang, nan ; jiang, xin] chinese acad sci, inst met res, shenyang natl lab mat sci, shenyang 110016, peoples r china ; [zhuang, hao ; jiang, xin] univ siegen, inst mat engn, d-57076 siegen, germany
KeywordZno Functionalization Coilalent Bond Electrochemistry Fluorescence Dna Biosensor
Funding OrganizationNational Natural Science Foundation of China [51202257]; Liaoning Province Doctoral Scientific Research Foundation of China [20131121]
Indexed Bysci
Language英语
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/74824
Collection中国科学院金属研究所
Corresponding Authorxjiang@imr.ac.cn
Recommended Citation
GB/T 7714
Wang, Chun,Huang, Nan,Zhuang, Hao,et al. Enhanced Performance of Nanocrystalline ZnO DNA Biosensor via Introducing Electrochemical Covalent Biolinkers[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(14):7605-7612.
APA Wang, Chun,Huang, Nan,Zhuang, Hao,Jiang, Xin,&xjiang@imr.ac.cn.(2015).Enhanced Performance of Nanocrystalline ZnO DNA Biosensor via Introducing Electrochemical Covalent Biolinkers.ACS APPLIED MATERIALS & INTERFACES,7(14),7605-7612.
MLA Wang, Chun,et al."Enhanced Performance of Nanocrystalline ZnO DNA Biosensor via Introducing Electrochemical Covalent Biolinkers".ACS APPLIED MATERIALS & INTERFACES 7.14(2015):7605-7612.
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