IMR OpenIR
Tantalum coating of porous carbon scaffold supplemented with autologous bone marrow stromal stem cells for bone regeneration in vitro and in vivo
Wei, Xiaowei; Zhao, Dewei; Wang, Benjie; Wang, Wei; Kang, Kai; Xie, Hui; Liu, Baoyi; Zhang, Xiuzhi; Zhang, Jinsong; Yang, Zhenming; zhaodewei2000@163.com
2016
发表期刊EXPERIMENTAL BIOLOGY AND MEDICINE
ISSN1535-3702
卷号241期号:6页码:592-602
摘要Porous tantalum metal with low elastic modulus is similar to cancellous bone. Reticulated vitreous carbon (RVC) can provide three-dimensional pore structure and serves as the ideal scaffold of tantalum coating. In this study, the biocompatibility of domestic porous tantalum was first successfully tested with bone marrow stromal stem cells (BMSCs) in vitro and for bone tissue repair in vivo. We evaluated cytotoxicity of RVC scaffold and tantalum coating using BMSCs. The morphology, adhesion, and proliferation of BMSCs were observed via laser scanning confocal microscope and scanning electron microscopy. In addition, porous tantalum rods with or without autologous BMSCs were implanted on hind legs in dogs, respectively. The osteogenic potential was observed by hard tissue slice examination. At three weeks and six weeks following implantation, new osteoblasts and new bone were observed at the tantalum-host bone interface and pores. At 12 weeks postporous tantalum with autologous BMSCs implantation, regenerated trabecular equivalent to mature bone was found in the pore of tantalum rods. Our results suggested that domestic porous tantalum had excellent biocompatibility and could promote new bone formation in vivo. Meanwhile, the osteogenesis of porous tantalum associated with autologous BMSCs was more excellent than only tantalum implantation. Future clinical studies are warranted to verify the clinical efficacy of combined implantation of this domestic porous tantalum associated with autologous BMSCs implantation and compare their efficacy with conventional autologous bone grafting carrying blood vessel in patients needing bone repairing.
部门归属[wei, xiaowei ; zhao, dewei ; wang, benjie ; wang, wei ; kang, kai ; xie, hui ; liu, baoyi ; zhang, xiuzhi] dalian univ, dept orthopaed lab, zhongshan hosp, dalian 116001, peoples r china ; [zhang, jinsong ; yang, zhenming] chinese acad sci, inst met res, shenyang 110016, peoples r china
关键词Bone Marrow Stromal Stem Cells Porous Tantalum Biocompatibility Osteogenesis Bone Tissue Engineering
资助者National Natural Science Foundation of China [81371942]; National Science and Technology Support Program [2012BAI17B02]; Dalian Science and Technology Bureau [2013J21DW004]; Dalian University [2015YBL003]
收录类别sci
语种英语
文献类型期刊论文
条目标识符http://ir.imr.ac.cn/handle/321006/75177
专题中国科学院金属研究所
通讯作者zhaodewei2000@163.com
推荐引用方式
GB/T 7714
Wei, Xiaowei,Zhao, Dewei,Wang, Benjie,et al. Tantalum coating of porous carbon scaffold supplemented with autologous bone marrow stromal stem cells for bone regeneration in vitro and in vivo[J]. EXPERIMENTAL BIOLOGY AND MEDICINE,2016,241(6):592-602.
APA Wei, Xiaowei.,Zhao, Dewei.,Wang, Benjie.,Wang, Wei.,Kang, Kai.,...&zhaodewei2000@163.com.(2016).Tantalum coating of porous carbon scaffold supplemented with autologous bone marrow stromal stem cells for bone regeneration in vitro and in vivo.EXPERIMENTAL BIOLOGY AND MEDICINE,241(6),592-602.
MLA Wei, Xiaowei,et al."Tantalum coating of porous carbon scaffold supplemented with autologous bone marrow stromal stem cells for bone regeneration in vitro and in vivo".EXPERIMENTAL BIOLOGY AND MEDICINE 241.6(2016):592-602.
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