The in vitro biocompatibility and macrophage phagocytosis of Mg17Al12 phase in Mg-Al-Zn alloys

IMR OpenIR

	The in vitro biocompatibility and macrophage phagocytosis of Mg17Al12 phase in Mg-Al-Zn alloys
	Liu, Chen; He, Peng; Wan, Peng; Li, Mei; Wang, Kehong; Tan, Lili; Zhang, Yu; Yang, Ke; luck_2001@126.com; kyang@imr.ac.cn
	2015
发表期刊	JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
ISSN	1549-3296
卷号	103 期号:7 页码:2405-2415
摘要	Mg alloys are gaining interest for applications as biodegradable medical implant, including Mg-Al-Zn series alloys with good combination of mechanical properties and reasonable corrosion resistance. However, whether the existence of second phase particles in the alloys exerts influence on the biocompatibility is still not clear. A deeper understanding of how the particles regulate specific biological responses is becoming a crucial requirement for their subsequent biomedical application. In this work, the in vitro biocompatibility of Mg17Al12 as a common second phase in biodegradable Mg-Al-Zn alloys was investigated via hemolysis, cytotoxicity, cell proliferation, and cell adhesion tests. Moreover, osteogenic differentiation was evaluated by the extracellular matrix mineralization assay. The Mg17Al12 particles were also prepared to simulate the real situation of second phase in the in vivo environment in order to estimate the cellular response in macrophages to the Mg17Al12 particles. The experimental results indicated that no hemolysis was found and an excellent cytocompatibility was also proved for the Mg17Al12 second phase when co-cultured with L929 cells, MC3T3-E1 cells and BMSCs. Macrophage phagocytosis co-culture test revealed that Mg17Al12 particles exerted no harmful effect on RAW264.7 macrophages and could be phagocytized by the RAW264.7 cells. Furthermore, the possible inflammatory reaction and metabolic way for Mg17Al12 phase were also discussed in detail. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103: 2405-2415, 2015.
部门归属	[liu, chen ; wang, kehong] nanjing univ sci & technol, dept mat sci & engn, nanjing 210094, jiangsu, peoples r china ; [liu, chen ; wan, peng ; tan, lili ; yang, ke] chinese acad sci, inst met res, shenyang 110016, peoples r china ; [he, peng] southern med univ, guangzhou 510515, guangdong, peoples r china ; [he, peng ; li, mei ; zhang, yu] guangzhou mil command, guangzhou gen hosp, orthoped hosp, guangzhou 510010, guangdong, peoples r china
关键词	Magnesium Alloys Second Phase Mg17al12 Biocompatibility Macrophage Phagocytosis
资助者	National Basic Research Program of China [2012CB619101]; National Natural Science Foundation of China [81401773]; Guangdong Key Laboratory of Enterprises for Biodegradable Mg alloys and Related Implant Device [2012A061600002]
收录类别	sci
语种	英语
WOS记录号	WOS:000355246400020
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/75196
专题	中国科学院金属研究所
通讯作者	luck_2001@126.com; kyang@imr.ac.cn
推荐引用方式 GB/T 7714	Liu, Chen,He, Peng,Wan, Peng,et al. The in vitro biocompatibility and macrophage phagocytosis of Mg17Al12 phase in Mg-Al-Zn alloys[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2015,103(7):2405-2415.
APA	Liu, Chen.,He, Peng.,Wan, Peng.,Li, Mei.,Wang, Kehong.,...&kyang@imr.ac.cn.(2015).The in vitro biocompatibility and macrophage phagocytosis of Mg17Al12 phase in Mg-Al-Zn alloys.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,103(7),2405-2415.
MLA	Liu, Chen,et al."The in vitro biocompatibility and macrophage phagocytosis of Mg17Al12 phase in Mg-Al-Zn alloys".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 103.7(2015):2405-2415.