|Toward a Molecular Understanding of the Antibacterial Mechanism of Copper-Bearing Titanium Alloys against Staphylococcus aureus|
|Li, Mei; Ma, Zheng; Zhu, Ye; Xia, Hong; Yao, Mengyu; Chu, Xiao; Wang, Xiaolan; Yang, Ke; Yang, Mingying; Zhang, Yu; Mao, Chuanbin; firstname.lastname@example.org; email@example.com; firstname.lastname@example.org
|Source Publication||ADVANCED HEALTHCARE MATERIALS
|Abstract||The antibacterial mechanism of the Cu-containing materials has not been fully understood although such understanding is crucial for the sustained clinical use of Cu-containing antibacterial materials such as bone implants. The aim of this study is to investigate the molecular mechanisms by which the Gram-positive Staphylococcus aureus is inactivated through Cu-bearing titanium alloys (Ti6Al4V5Cu). Cu ions released from the alloys are found to contribute to lethal damage of bacteria. They destroy the permeability of the bacterial membranes, resulting in the leakage of reducing sugars and proteins from the cells. They also promote the generation of bacteria-killing reactive oxygen species (ROS). The ROS production is confirmed by several assays including fluorescent staining of intracellular oxidative stress, detection of respiratory chain activity, and measurement of the levels of lipid peroxidation, catalase, and glutathione. Furthermore, the released Cu ions show obvious genetic toxicity by interfering the replication of nuc (species-specific) and 16SrRNA genes, but with no effect on the genome integrity. All of these effects lead to the antibacterial effect of Ti6Al4V5Cu. Collectively, our work reconciles the conflicting antibacterial mechanisms of Cu-bearing metallic materials or nanoparticles reported in the literature and highlights the potential use of Ti6Al4V5Cu alloys in inhibiting bacterial infections.|
; xia, hong
; yao, mengyu
; chu, xiao
; wang, xiaolan
; zhang, yu] guangzhou mil command, guangzhou gen hosp, dept orthoped, guangdong key lab orthopaed technol & implant mat, guangzhou 510010, guangdong, peoples r china
; [li, mei] xi an jiao tong univ, state key lab mech behav mat, xian 710049, shaanxi, peoples r china
; [ma, zheng
; yang, ke] chinese acad sci, inst met res, shenyang 110016, liaoning, peoples r china
; [zhu, ye
; mao, chuanbin] univ oklahoma, dept chem & biochem, stephenson life sci res ctr, norman, ok 73019 usa
; [yang, mingying] zhejiang univ, coll anim sci, inst appl bioresource res, yuhangtang rd 866, hangzhou 310058, zhejiang, peoples r china
; [mao, chuanbin] zhejiang univ, sch mat sci & engn, hangzhou 310027, zhejiang, peoples r china
|Funding Organization||National Natural Science Foundation of China [81271957, 81501859, 81272057, 21172194]; National Basic Research Program of China [2012CB 619106]; Projects of Zhejiang Provincial Science and Technology Plans [2012C12910]; Silkworm Industry Science and Technology Innovation Team [2011R50028]; China Agriculture Research System [CARS-22-ZJ0402]; National High Technology Research and Development Program 863 [2013AA102507]; National Institutes of Health [EB015190, CA200504]; National Science Foundation [CMMI-1234957, CBET-1512664]; Department of Defense Congressionally Directed Medical Research Program [W81XWH-15-1-0180]; Oklahoma Center for Adult Stem Cell Research ; Oklahoma Center for the Advancement of Science and Technology [HR14-160]
|Corresponding Authoremail@example.com; firstname.lastname@example.org; email@example.com|
Li, Mei,Ma, Zheng,Zhu, Ye,et al. Toward a Molecular Understanding of the Antibacterial Mechanism of Copper-Bearing Titanium Alloys against Staphylococcus aureus[J]. ADVANCED HEALTHCARE MATERIALS,2016,5(5):557-566.
Li, Mei.,Ma, Zheng.,Zhu, Ye.,Xia, Hong.,Yao, Mengyu.,...&firstname.lastname@example.org.(2016).Toward a Molecular Understanding of the Antibacterial Mechanism of Copper-Bearing Titanium Alloys against Staphylococcus aureus.ADVANCED HEALTHCARE MATERIALS,5(5),557-566.
Li, Mei,et al."Toward a Molecular Understanding of the Antibacterial Mechanism of Copper-Bearing Titanium Alloys against Staphylococcus aureus".ADVANCED HEALTHCARE MATERIALS 5.5(2016):557-566.
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