|An investigation of the antibacterial ability and cytotoxicity of a novel cu-bearing 317L stainless steel|
|Sun, Da; Xu, Dake; Yang, Chunguang; Shahzad, M. Babar; Sun, Ziqing; Xia, Jin; Zhao, Jinlong; Gu, Tingyue; Yang, Ke; Wang, Guixue; Wang, GX (reprint author), Chongqing Univ, Coll Bioengn, Key Lab Biorheol Sci & Technol, Chongqing 400044, Peoples R China.; Yang, K (reprint author), Chinese Acad Sci, Inst Met Res, 72 Wenhua Rd, Shenyang 110016, Peoples R China.
|摘要||In order to solve the challenging problem of microbial infections caused by microorganisms on medical implants, it is imperative to develop novel antimicrobial biomaterials. This work demonstrated that 317L-Cu stainless steel (SS), created by adding copper through a solution and aging heat treatment process, exhibited good antibacterial properties against staphylococcus aureus, achieving 2 log reduction of planktonic cells after 5 days of incubation. In this study, the antibacterial test was performed using the plate count method, the fluorescence cell staining method and the quantitative polymerase chain reaction (qPCR) method. It is well known that a high concentration of copper ion can lead to cytotoxicity. This work explored the cytotoxicity of 317L-Cu SS through real-time cell analysis (RTCA). Experimental results demonstrated that the 317L-Cu SS possessed a satisfactory antibacterial ability against S. aureus, and the antibacterial rate based on the reduction of sessile cell count reached 98.3% after 24-hour treatment. The bacterial adhesion and the biofilm thickness were considerably reduced by the 317L-Cu SS. The results of RTCA suggested that 317L-Cu SS did not introduce cytotoxicity to mouse cells, indicating its suitability as a medical implant material.|
; wang, guixue] chongqing univ, coll bioengn, key lab biorheol sci & technol, chongqing 400044, peoples r china
; [xu, dake
; yang, chunguang
; shahzad, m. babar
; sun, ziqing
; zhao, jinlong
; yang, ke] chinese acad sci, inst met res, 72 wenhua rd, shenyang 110016, peoples r china
; [xia, jin] liaoning univ, coll chem, shenyang 110036, peoples r china
; [gu, tingyue] ohio univ, inst corros & multiphase technol, dept chem & biomol engn, athens, oh 45701 usa
|学科领域||Science & Technology - Other Topics
|资助者||National Natural Science Foundation of China ; National Key Technology R & D Program of China [2012BAJ25B09]; "Young Merit Scholars" program of the Institute of Metal Research, Chinese Academy of Sciences; Chongqing Engineering Laboratory in Vascular Implants; National "111 Plan" Base [B06023]; Public Experiment Center of State Bioindustrial Base (Chongqing) of China
|通讯作者||Wang, GX (reprint author), Chongqing Univ, Coll Bioengn, Key Lab Biorheol Sci & Technol, Chongqing 400044, Peoples R China.; Yang, K (reprint author), Chinese Acad Sci, Inst Met Res, 72 Wenhua Rd, Shenyang 110016, Peoples R China.|
Sun, Da,Xu, Dake,Yang, Chunguang,et al. An investigation of the antibacterial ability and cytotoxicity of a novel cu-bearing 317L stainless steel[J]. SCIENTIFIC REPORTS,2016,6.
Sun, Da.,Xu, Dake.,Yang, Chunguang.,Shahzad, M. Babar.,Sun, Ziqing.,...&Yang, K .(2016).An investigation of the antibacterial ability and cytotoxicity of a novel cu-bearing 317L stainless steel.SCIENTIFIC REPORTS,6.
Sun, Da,et al."An investigation of the antibacterial ability and cytotoxicity of a novel cu-bearing 317L stainless steel".SCIENTIFIC REPORTS 6(2016).