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Biological behaviour of human umbilical artery smooth muscle cell grown on nickel-free and nickel-containing stainless steel for stent implantation
Li, Liming; An, Liwen; Zhou, Xiaohang; Pan, Shuang; Meng, Xin; Ren, Yibin; Yang, Ke; Guan, Yifu; Li, LM (reprint author), Northeastern Univ, Inst Biotechnol, Shenyang, Peoples R China.; Li, LM (reprint author), China Med Univ, Dept Biochem & Mol Biol, Shenyang 110001, Peoples R China.
2016-01-04
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume6
AbstractTo evaluate the clinical potential of high nitrogen nickel-free austenitic stainless steel (HNNF SS), we have compared the cellular and molecular responses of human umbilical artery smooth muscle cells (HUASMCs) to HNNF SS and 316L SS (nickel-containing austenitic 316L stainless steel). CCK-8 analysis and flow cytometric analysis were used to assess the cellular responses (proliferation, apoptosis, and cell cycle), and quantitative real-time PCR (qRT-PCR) was used to analyze the gene expression profiles of HUASMCs exposed to HNNF SS and 316L SS, respectively. CCK-8 analysis demonstrated that HUASMCs cultured on HNNF SS proliferated more slowly than those on 316L SS. Flow cytometric analysis revealed that HNNF SS could activate more cellular apoptosis. The qRT-PCR results showed that the genes regulating cell apoptosis and autophagy were up-regulated on HNNF SS. Thus, HNNF SS could reduce the HUASMC proliferation in comparison to 316L SS. The findings furnish valuable information for developing new biomedical materials for stent implantation.
description.department[li, liming] northeastern univ, inst biotechnol, shenyang, peoples r china ; [li, liming ; an, liwen ; zhou, xiaohang ; pan, shuang ; meng, xin ; guan, yifu] china med univ, dept biochem & mol biol, shenyang 110001, peoples r china ; [ren, yibin ; yang, ke] chinese acad sci, inst met res, shenyang 110016, peoples r china
Subject AreaScience & Technology - Other Topics
Funding OrganizationNational Natural Science Foundation of China [31070705]; Doctoral Startup Foundation of Liaoning Province [20141010]
Indexed Bysci
Language英语
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/75948
Collection中国科学院金属研究所
Corresponding AuthorLi, LM (reprint author), Northeastern Univ, Inst Biotechnol, Shenyang, Peoples R China.; Li, LM (reprint author), China Med Univ, Dept Biochem & Mol Biol, Shenyang 110001, Peoples R China.
Recommended Citation
GB/T 7714
Li, Liming,An, Liwen,Zhou, Xiaohang,et al. Biological behaviour of human umbilical artery smooth muscle cell grown on nickel-free and nickel-containing stainless steel for stent implantation[J]. SCIENTIFIC REPORTS,2016,6.
APA Li, Liming.,An, Liwen.,Zhou, Xiaohang.,Pan, Shuang.,Meng, Xin.,...&Li, LM .(2016).Biological behaviour of human umbilical artery smooth muscle cell grown on nickel-free and nickel-containing stainless steel for stent implantation.SCIENTIFIC REPORTS,6.
MLA Li, Liming,et al."Biological behaviour of human umbilical artery smooth muscle cell grown on nickel-free and nickel-containing stainless steel for stent implantation".SCIENTIFIC REPORTS 6(2016).
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