Biological behaviour of human umbilical artery smooth muscle cell grown on nickel-free and nickel-containing stainless steel for stent implantation

IMR OpenIR

	Biological behaviour of human umbilical artery smooth muscle cell grown on nickel-free and nickel-containing stainless steel for stent implantation
	Li, Liming; An, Liwen; Zhou, Xiaohang; Pan, Shuang; Meng, Xin; Ren, Yibin; Yang, Ke; Guan, Yifu; Li, LM (reprint author), Northeastern Univ, Inst Biotechnol, Shenyang, Peoples R China.; Li, LM (reprint author), China Med Univ, Dept Biochem & Mol Biol, Shenyang 110001, Peoples R China.
	2016-01-04
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	6
摘要	To evaluate the clinical potential of high nitrogen nickel-free austenitic stainless steel (HNNF SS), we have compared the cellular and molecular responses of human umbilical artery smooth muscle cells (HUASMCs) to HNNF SS and 316L SS (nickel-containing austenitic 316L stainless steel). CCK-8 analysis and flow cytometric analysis were used to assess the cellular responses (proliferation, apoptosis, and cell cycle), and quantitative real-time PCR (qRT-PCR) was used to analyze the gene expression profiles of HUASMCs exposed to HNNF SS and 316L SS, respectively. CCK-8 analysis demonstrated that HUASMCs cultured on HNNF SS proliferated more slowly than those on 316L SS. Flow cytometric analysis revealed that HNNF SS could activate more cellular apoptosis. The qRT-PCR results showed that the genes regulating cell apoptosis and autophagy were up-regulated on HNNF SS. Thus, HNNF SS could reduce the HUASMC proliferation in comparison to 316L SS. The findings furnish valuable information for developing new biomedical materials for stent implantation.
部门归属	[li, liming] northeastern univ, inst biotechnol, shenyang, peoples r china ; [li, liming ; an, liwen ; zhou, xiaohang ; pan, shuang ; meng, xin ; guan, yifu] china med univ, dept biochem & mol biol, shenyang 110001, peoples r china ; [ren, yibin ; yang, ke] chinese acad sci, inst met res, shenyang 110016, peoples r china
学科领域	Science & Technology - Other Topics
资助者	National Natural Science Foundation of China [31070705]; Doctoral Startup Foundation of Liaoning Province [20141010]
收录类别	sci
语种	英语
WOS记录号	WOS:000373733900001
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/75948
专题	中国科学院金属研究所
通讯作者	Li, LM (reprint author), Northeastern Univ, Inst Biotechnol, Shenyang, Peoples R China.; Li, LM (reprint author), China Med Univ, Dept Biochem & Mol Biol, Shenyang 110001, Peoples R China.
推荐引用方式 GB/T 7714	Li, Liming,An, Liwen,Zhou, Xiaohang,et al. Biological behaviour of human umbilical artery smooth muscle cell grown on nickel-free and nickel-containing stainless steel for stent implantation[J]. SCIENTIFIC REPORTS,2016,6.
APA	Li, Liming.,An, Liwen.,Zhou, Xiaohang.,Pan, Shuang.,Meng, Xin.,...&Li, LM .(2016).Biological behaviour of human umbilical artery smooth muscle cell grown on nickel-free and nickel-containing stainless steel for stent implantation.SCIENTIFIC REPORTS,6.
MLA	Li, Liming,et al."Biological behaviour of human umbilical artery smooth muscle cell grown on nickel-free and nickel-containing stainless steel for stent implantation".SCIENTIFIC REPORTS 6(2016).