Biodegradable Mg-Cu alloy implants with antibacterial activity for the treatment of osteomyelitis: In vitro and in vivo evaluations

IMR OpenIR

	Biodegradable Mg-Cu alloy implants with antibacterial activity for the treatment of osteomyelitis: In vitro and in vivo evaluations
	Li, Y; Liu, LN; Wan, P; Zhai, ZJ; Mao, ZY; Ouyang, ZX; Yu, DG; Sun, Q; Tan, LL; Ren, L; Zhu, ZA; Hao, YQ; Qu, XH; Yang, K; Dai, KR; Dai, KR (reprint author), Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Orthopaed Surg, Shanghai Key Lab Orthopaed Implants,Sch Med, 639 Zhizaoju Rd, Shanghai 200011, Peoples R China.; Yang, K (reprint author), Chinese Acad Sci, Inst Met Res, 72 Wenhua Rd, Shenyang 110016, Peoples R China.
	2016-11-01
发表期刊	BIOMATERIALS
ISSN	0142-9612
卷号	106 页码:250-263
摘要	Treatment of chronic osteomyelitis (bone infection) remains a clinical challenge; in particular, it requires an implantable material with improved antibacterial activity. Here, we prepared biodegradable magnesium (Mg)-copper (Cu) alloys with different Cu contents (0.05, 0.1, and 0.25 wt%) and assessed their potential for treating methicillin-resistant Staphylococcus aureus-induced osteomyelitis. We evaluated the microstructures, mechanical properties, corrosion behavior, and ion release of the alloys in vitro, and their biocompatibility and antibacterial activity in vitro and in vivo. The antibacterial activity of the Mg Cu alloys in vitro was demonstrated by microbiological counting assays, bacterial viability assays, biofilm formation observations, and the expression of biofilm, virulence, and antibiotic-resistance associated genes. The antibacterial activity of Mg-Cu alloys in vivo was confirmed by imaging examination, microbiological cultures, and histopathology. The biocompatibility of Mg-Cu alloys was confirmed by cell proliferation, vitality, and morphology assays in vitro and Cu2+ or Mg2+ ion assays, blood biochemical tests, and histological evaluation in vivo. The alloy containing 0.25 wt% Cu exhibited the highest antibacterial activity among the tested alloys, with favorable biocompatibility. Collectively, our results indicate the potential utility of Mg -Cu alloy implants with 0.25 wt% Cu in treating orthopedic infections. (C) 2016 Elsevier Ltd. All rights reserved.
部门归属	[li, yang ; zhai, zanjing ; mao, zhenyang ; ouyang, zhengxiao ; yu, degang ; zhu, zhenan ; hao, yongqiang ; qu, xinhua ; dai, kerong] shanghai jiao tong univ, shanghai peoples hosp 9, dept orthopaed surg, shanghai key lab orthopaed implants,sch med, 639 zhizaoju rd, shanghai 200011, peoples r china ; [liu, lina] shanghai jiao tong univ, shanghai peoples hosp 9, dept plast & reconstruct surg, sch med, shanghai, peoples r china ; [wan, peng ; tan, lili ; ren, ling ; yang, ke] chinese acad sci, inst met res, 72 wenhua rd, shenyang 110016, peoples r china ; [sun, qi] shanghai jiao tong univ, sch med, shanghai peoples hosp 9, dept radiol, shanghai, peoples r china
关键词	Osteomyelitis Antibacterial Activity Biodegradable Mg-cu Alloy
学科领域	Engineering, Biomedical ; Materials Science, bioMaterials
资助者	Key National Basic Research Program of China [2012CB619101, 2015AA033603]; National Natural Science Foundation of China [81190133]; National Natural Science Foundation for Youths [81401852, 81401773, 31500777]; Doctoral Innovation Fund Projects from Shanghai Jiao Tong University's School of Medicine [BXJ201430]; Natural Science Foundation of Shanghai [14ZR1424000]; "Chen Guang" Project of the Shanghai Municipal Education Commission; Shanghai Education Development Foundation [14CG14]
收录类别	sci
语种	英语
WOS记录号	WOS:000383934300021
引用统计	被引频次：227[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/76216
专题	中国科学院金属研究所
通讯作者	Qu, XH; Dai, KR (reprint author), Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Orthopaed Surg, Shanghai Key Lab Orthopaed Implants,Sch Med, 639 Zhizaoju Rd, Shanghai 200011, Peoples R China.; Yang, K (reprint author), Chinese Acad Sci, Inst Met Res, 72 Wenhua Rd, Shenyang 110016, Peoples R China.
推荐引用方式 GB/T 7714	Li, Y,Liu, LN,Wan, P,et al. Biodegradable Mg-Cu alloy implants with antibacterial activity for the treatment of osteomyelitis: In vitro and in vivo evaluations[J]. BIOMATERIALS,2016,106:250-263.
APA	Li, Y.,Liu, LN.,Wan, P.,Zhai, ZJ.,Mao, ZY.,...&Yang, K .(2016).Biodegradable Mg-Cu alloy implants with antibacterial activity for the treatment of osteomyelitis: In vitro and in vivo evaluations.BIOMATERIALS,106,250-263.
MLA	Li, Y,et al."Biodegradable Mg-Cu alloy implants with antibacterial activity for the treatment of osteomyelitis: In vitro and in vivo evaluations".BIOMATERIALS 106(2016):250-263.