Osteoblast cellular activity on low elastic modulus Ti-24Nb-4Zr-8Sn alloy

IMR OpenIR

	Osteoblast cellular activity on low elastic modulus Ti-24Nb-4Zr-8Sn alloy
	Nune, K. C.; Misra, R. D. K.; Li, S. J.; Hao, Y. L.; Yang, R.; Misra, RDK (reprint author), Univ Texas El Paso, Dept Met Mat & Biomed Engn, 500 W Univ Ave, El Paso, TX 79968 USA.
	2017-02-01
发表期刊	DENTAL MATERIALS
ISSN	0109-5641
卷号	33 期号:2 页码:152-165
摘要	Objectives. Low modulus p-titanium alloys with non-toxic alloying elements are envisaged to provide good biocompatibility and alleviate the undesired stress shielding effect. The objective of this study is to fundamentally elucidate the biological response of novel high strength-low elastic modulus Ti2448 alloy through the study of bioactivity and osteoblast cell functions. Methods. Characterization techniques such as SEM, EDX, XRD, and fluorescence microscopy were utilized to analyze the microstructure, morphology, chemical composition, and cell adhesion. The cellular activity was explored in terms of cell-to-cell communication involving proliferation, spreading, synthesis of extracellular and intracellular proteins, differentiation, and mineralization. Results. The formation of fine apatite-like crystals on the surface during immersion test in simulated body fluid confirmed the bioactivity of the surface, which provided the favorable osteogenic microenvironment for cell-material interaction. The proliferation and differentiation of pre-osteoblasts and their ability to form a well mineralized bone-like extracellular matrix (ECM) by secreting bone markers (ALP, calcium, etc.) over the surface point toward the determining role of unique surface chemistry and surface properties of the Ti-24Nb-4Zr-8Sn (Ti2448) alloy in modulating osteoblasts functions. Significance. These results demonstrated that the low modulus (similar to 49 GPa) Ti2448 alloy with non-toxic alloying elements can be used as a potential dental or orthopedic load-bearing implant material. (C) 2016 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.; Objectives. Low modulus p-titanium alloys with non-toxic alloying elements are envisaged to provide good biocompatibility and alleviate the undesired stress shielding effect. The objective of this study is to fundamentally elucidate the biological response of novel high strength-low elastic modulus Ti2448 alloy through the study of bioactivity and osteoblast cell functions. Methods. Characterization techniques such as SEM, EDX, XRD, and fluorescence microscopy were utilized to analyze the microstructure, morphology, chemical composition, and cell adhesion. The cellular activity was explored in terms of cell-to-cell communication involving proliferation, spreading, synthesis of extracellular and intracellular proteins, differentiation, and mineralization. Results. The formation of fine apatite-like crystals on the surface during immersion test in simulated body fluid confirmed the bioactivity of the surface, which provided the favorable osteogenic microenvironment for cell-material interaction. The proliferation and differentiation of pre-osteoblasts and their ability to form a well mineralized bone-like extracellular matrix (ECM) by secreting bone markers (ALP, calcium, etc.) over the surface point toward the determining role of unique surface chemistry and surface properties of the Ti-24Nb-4Zr-8Sn (Ti2448) alloy in modulating osteoblasts functions. Significance. These results demonstrated that the low modulus (similar to 49 GPa) Ti2448 alloy with non-toxic alloying elements can be used as a potential dental or orthopedic load-bearing implant material. (C) 2016 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
部门归属	[nune, k. c. ; misra, r. d. k.] univ texas el paso, dept met mat & biomed engn, biomat & biomed engn res lab, 500 w univ ave, el paso, tx 79968 usa ; [li, s. j. ; hao, y. l. ; yang, r.] chinese acad sci, inst met res, shenyang natl lab mat sci, 72 wenhua rd, shenyang 110016, peoples r china
关键词	Ti-24nb-4zr-8sn Alloy Low Elastic Modulus Osteoblasts Functions
学科领域	Dentistry, Oral Surgery & Medicine ; Materials Science, bioMaterials
资助者	Chinese MoST [2016YFC1102601, 2015AA033702]; National Natural Science Foundation of China [51271180]; University of Texas at El Paso
收录类别	SCI
语种	英语
WOS记录号	WOS:000396409800008
引用统计	被引频次：62[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/78319
专题	中国科学院金属研究所
通讯作者	Misra, RDK (reprint author), Univ Texas El Paso, Dept Met Mat & Biomed Engn, 500 W Univ Ave, El Paso, TX 79968 USA.
推荐引用方式 GB/T 7714	Nune, K. C.,Misra, R. D. K.,Li, S. J.,et al. Osteoblast cellular activity on low elastic modulus Ti-24Nb-4Zr-8Sn alloy[J]. DENTAL MATERIALS,2017,33(2):152-165.
APA	Nune, K. C.,Misra, R. D. K.,Li, S. J.,Hao, Y. L.,Yang, R.,&Misra, RDK .(2017).Osteoblast cellular activity on low elastic modulus Ti-24Nb-4Zr-8Sn alloy.DENTAL MATERIALS,33(2),152-165.
MLA	Nune, K. C.,et al."Osteoblast cellular activity on low elastic modulus Ti-24Nb-4Zr-8Sn alloy".DENTAL MATERIALS 33.2(2017):152-165.