In vitro and in vivo evaluation of MgF2 coated AZ31 magnesium alloy porous scaffolds for bone regeneration

IMR OpenIR

	In vitro and in vivo evaluation of MgF2 coated AZ31 magnesium alloy porous scaffolds for bone regeneration
	Yu, Weilin; Zhao, Huakun; Ding, Zhenyu; Zhang, Zhiwang; Sun, Benben; Shen, Ji; Chen, Shanshan; Zhang, Bingchun; Yang, Ke; Liu, Meixia; Chen, Daoyun; He, Yaohua; Chen, DY; He, YH (reprint author), Shanghai Jiao Tong Univ, Dept Orthoped, Affiliated Peoples Hosp 6, Shanghai 200233, Peoples R China.; Liu, MX (reprint author), Chinese Acad Sci, Inst Met Res, Shenyang 110016, Peoples R China.
	2017
发表期刊	COLLOIDS AND SURFACES B-BIOINTERFACES
ISSN	0927-7765
卷号	149 页码:330-340
摘要	Porous magnesium scaffolds are attracting increasing attention because of their degradability and good mechanical property. In this work, a porous and degradable AZ31 magnesium alloy scaffold was fabricated using laser perforation technique. To enhance the corrosion resistance and cytocompatibility of the AZ31 scaffolds, a fluoride treatment was used to acquire the MgF2 coating. Enhanced corrosion resistance was confirmed by immersion and electrochemical tests. Due to the protection provided by the MgF2 coating, the magnesium release and pH increase resulting from the degradation of the FAZ31 scaffolds were controllable. Moreover, in vitro studies revealed that the MgF2 coated AZ31 (FAZ31) scaffolds enhanced the proliferation and attachment of rat bone marrow stromal cells (rBMSCs) compared with the AZ31 scaffolds. In addition, our present data indicated that the extract of the FAZ31 scaffold could enhance the osteogenic differentiation of rBMSCs. To compare the in vivo bone regenerative capacity of the AZ31 and FAZ31 scaffolds, a rabbit femoral condyle defect model was used. Micro-computed tomography (micro-CT) and histological examination were performed to evaluate the degradation of the scaffolds and bone volume changes. In addition to the enhanced the corrosion resistance, the FAZ31 scaffolds were more biocompatible and induced significantly more new bone formation in vivo. Conversely, bone resorption was observed from the AZ31 scaffolds. These promising results suggest potential clinical applications of the fluoride pretreated AZ31 scaffold for bone tissue repair and regeneration. (C) 2016 Elsevier B.V. All rights reserved.; Porous magnesium scaffolds are attracting increasing attention because of their degradability and good mechanical property. In this work, a porous and degradable AZ31 magnesium alloy scaffold was fabricated using laser perforation technique. To enhance the corrosion resistance and cytocompatibility of the AZ31 scaffolds, a fluoride treatment was used to acquire the MgF2 coating. Enhanced corrosion resistance was confirmed by immersion and electrochemical tests. Due to the protection provided by the MgF2 coating, the magnesium release and pH increase resulting from the degradation of the FAZ31 scaffolds were controllable. Moreover, in vitro studies revealed that the MgF2 coated AZ31 (FAZ31) scaffolds enhanced the proliferation and attachment of rat bone marrow stromal cells (rBMSCs) compared with the AZ31 scaffolds. In addition, our present data indicated that the extract of the FAZ31 scaffold could enhance the osteogenic differentiation of rBMSCs. To compare the in vivo bone regenerative capacity of the AZ31 and FAZ31 scaffolds, a rabbit femoral condyle defect model was used. Micro-computed tomography (micro-CT) and histological examination were performed to evaluate the degradation of the scaffolds and bone volume changes. In addition to the enhanced the corrosion resistance, the FAZ31 scaffolds were more biocompatible and induced significantly more new bone formation in vivo. Conversely, bone resorption was observed from the AZ31 scaffolds. These promising results suggest potential clinical applications of the fluoride pretreated AZ31 scaffold for bone tissue repair and regeneration. (C) 2016 Elsevier B.V. All rights reserved.
部门归属	[yu, weilin ; zhao, huakun ; ding, zhenyu ; zhang, zhiwang ; sun, benben ; shen, ji ; chen, daoyun ; he, yaohua] shanghai jiao tong univ, dept orthoped, affiliated peoples hosp 6, shanghai 200233, peoples r china ; [chen, shanshan ; zhang, bingchun ; yang, ke ; liu, meixia] chinese acad sci, inst met res, shenyang 110016, peoples r china
关键词	Magnesium Alloy Scaffold Surface Modification Mgf2 Coating Osteogenesis
学科领域	Biophysics ; Chemistry, Physical ; Materials Science, bioMaterials
资助者	National Natural Science Foundation of China [81271961, 81572106, 81271998]; Shanghai Committee of Science and Technology, China [15ZR1431900]
收录类别	SCI
语种	英语
WOS记录号	WOS:000390625200040
引用统计	被引频次：73[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/78380
专题	中国科学院金属研究所
通讯作者	Chen, DY; He, YH (reprint author), Shanghai Jiao Tong Univ, Dept Orthoped, Affiliated Peoples Hosp 6, Shanghai 200233, Peoples R China.; Liu, MX (reprint author), Chinese Acad Sci, Inst Met Res, Shenyang 110016, Peoples R China.
推荐引用方式 GB/T 7714	Yu, Weilin,Zhao, Huakun,Ding, Zhenyu,et al. In vitro and in vivo evaluation of MgF2 coated AZ31 magnesium alloy porous scaffolds for bone regeneration[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2017,149:330-340.
APA	Yu, Weilin.,Zhao, Huakun.,Ding, Zhenyu.,Zhang, Zhiwang.,Sun, Benben.,...&Liu, MX .(2017).In vitro and in vivo evaluation of MgF2 coated AZ31 magnesium alloy porous scaffolds for bone regeneration.COLLOIDS AND SURFACES B-BIOINTERFACES,149,330-340.
MLA	Yu, Weilin,et al."In vitro and in vivo evaluation of MgF2 coated AZ31 magnesium alloy porous scaffolds for bone regeneration".COLLOIDS AND SURFACES B-BIOINTERFACES 149(2017):330-340.