Effect of low-intensity pulsed ultrasound on the biological behavior of osteoblasts on porous titanium alloy scaffolds: An in vitro and in vivo study

IMR OpenIR

	Effect of low-intensity pulsed ultrasound on the biological behavior of osteoblasts on porous titanium alloy scaffolds: An in vitro and in vivo study
	Cao, Hongjuan; Feng, Lifang; Wu, Zhenxian; Hou, Wentao; Li, Shujun; Hao, Yulin; Wu, Lin; Wu, L (reprint author), China Med Univ, Sch Stomatol, Dept Prosthodont, Shenyang, Liaoning, Peoples R China.
	2017-11-01
发表期刊	ELSEVIER SCIENCE BV
ISSN	0928-4931
卷号	80 页码:7-17
摘要	Low-intensity pulsed ultrasound (LIPUS) has been used in patients with fresh fractures, delayed union and nonunion to enhance bone healing and improve functional outcome. However, there were few studies concerning the effects of LIPUS on the biological behavior of osteoblasts on porous scaffolds. This study aimed to evaluate the effects of LIPUS on the biological behavior of osteoblasts on porous titanium-6aluminum-4vanadium (Ti6A14V) alloy scaffolds in vitro and in vivo. Scaffolds were randomly divided into an ultrasound group and a control group. Mouse pre-osteoblast cells were cultured with porous Ti6AI4V scaffolds in vitro. The effects of LIPUS on the biological behavior of osteoblasts were evaluated by observing the adhesion, proliferation, differentiation and ingrowth depth on porous Ti6A14V scaffolds. In addition, scaffolds were implanted into rabbit mandibular defects in vivo. The effects of LIPUS on bone regeneration were evaluated via micro-CT, fluorescent staining and toluidine blue staining. The results revealed that osteoblast adhered well to the scaffolds, and there was no significant difference in the methyl thiazolyl tetrazolium value between the ultrasound group and the control group (p > 0.05). Compared with the control group, ultrasound promoted the alkaline phosphatase activity, osteocalcin levels and ingrowth depth of the cells on the scaffolds (p < 0.05). In addition, micro-CT and histomorphological analysis showed that the volume and amount of new bone formation were increased and that bone maturity was improved in the ultrasound group compared to the control group. These results indicate that LIPUS promotes osteoblast differentiation as well as enhances bone ingrowth in porous Ti6AI4V scaffolds, and promotes bone formation and maturity in porous Ti6A14V scaffolds. (C) 2017 Elsevier B.V. All rights reserved.; Low-intensity pulsed ultrasound (LIPUS) has been used in patients with fresh fractures, delayed union and nonunion to enhance bone healing and improve functional outcome. However, there were few studies concerning the effects of LIPUS on the biological behavior of osteoblasts on porous scaffolds. This study aimed to evaluate the effects of LIPUS on the biological behavior of osteoblasts on porous titanium-6aluminum-4vanadium (Ti6A14V) alloy scaffolds in vitro and in vivo. Scaffolds were randomly divided into an ultrasound group and a control group. Mouse pre-osteoblast cells were cultured with porous Ti6AI4V scaffolds in vitro. The effects of LIPUS on the biological behavior of osteoblasts were evaluated by observing the adhesion, proliferation, differentiation and ingrowth depth on porous Ti6A14V scaffolds. In addition, scaffolds were implanted into rabbit mandibular defects in vivo. The effects of LIPUS on bone regeneration were evaluated via micro-CT, fluorescent staining and toluidine blue staining. The results revealed that osteoblast adhered well to the scaffolds, and there was no significant difference in the methyl thiazolyl tetrazolium value between the ultrasound group and the control group (p > 0.05). Compared with the control group, ultrasound promoted the alkaline phosphatase activity, osteocalcin levels and ingrowth depth of the cells on the scaffolds (p < 0.05). In addition, micro-CT and histomorphological analysis showed that the volume and amount of new bone formation were increased and that bone maturity was improved in the ultrasound group compared to the control group. These results indicate that LIPUS promotes osteoblast differentiation as well as enhances bone ingrowth in porous Ti6AI4V scaffolds, and promotes bone formation and maturity in porous Ti6A14V scaffolds. (C) 2017 Elsevier B.V. All rights reserved.
部门归属	[cao, hongjuan ; feng, lifang ; wu, zhenxian ; wu, lin] china med univ, sch stomatol, dept prosthodont, shenyang, liaoning, peoples r china ; [hou, wentao ; li, shujun ; hao, yulin] chinese acad sci, inst met res, shenyang natl lab mat sci, shenyang, liaoning, peoples r china
关键词	Low Intensity Pulsed Ultrasound (Lipus) Porous Ti6ai4v Scaffolds Osteoblasts Biological Behavior Bone Formation
学科领域	Materials Science, bioMaterials
资助者	National High Technology Research & Development Program (863 Program) of China [2015AA033702]; Natural Science Foundation of Liaoning Province, China [2014021008]
收录类别	SCI
语种	英语
WOS记录号	WOS:000410254400002
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/79023
专题	中国科学院金属研究所
通讯作者	Wu, L (reprint author), China Med Univ, Sch Stomatol, Dept Prosthodont, Shenyang, Liaoning, Peoples R China.
推荐引用方式 GB/T 7714	Cao, Hongjuan,Feng, Lifang,Wu, Zhenxian,et al. Effect of low-intensity pulsed ultrasound on the biological behavior of osteoblasts on porous titanium alloy scaffolds: An in vitro and in vivo study[J]. ELSEVIER SCIENCE BV,2017,80:7-17.
APA	Cao, Hongjuan.,Feng, Lifang.,Wu, Zhenxian.,Hou, Wentao.,Li, Shujun.,...&Wu, L .(2017).Effect of low-intensity pulsed ultrasound on the biological behavior of osteoblasts on porous titanium alloy scaffolds: An in vitro and in vivo study.ELSEVIER SCIENCE BV,80,7-17.
MLA	Cao, Hongjuan,et al."Effect of low-intensity pulsed ultrasound on the biological behavior of osteoblasts on porous titanium alloy scaffolds: An in vitro and in vivo study".ELSEVIER SCIENCE BV 80(2017):7-17.