IMR OpenIR
Characterization of synergistic anti-tumor effects of doxorubicin and p53 via graphene oxide-polyethyleneimine nanocarriers
Xie, Bei; Yi, Jipeng; Peng, Jian; Zhang, Xing; Lei, Lei; Zhao, Dapeng; Lei, Zhixin; Nie, Hemin; Nie, HM (reprint author), Hunan Univ, Inst Bionanotechnol & Tissue Engn, Coll Life Sci, Changsha 410082, Hunan, Peoples R China.; Zhang, X (reprint author), Chinese Acad Sci, Shenyang Natl Lab Mat Sci, Inst Met Res, Shenyang 110016, Liaoning, Peoples R China.
2017-08-01
发表期刊JOURNAL MATER SCI TECHNOL
ISSN1005-0302
卷号33期号:8页码:807-814
摘要Co-delivery of chemical drugs and therapeutic genes for synergistic therapy provides a promising strategy to treat devastating diseases. However, the real-time coordination patterns between chemical drugs and therapeutic genes remain poorly understood. Herein, the complexes of doxorubicin/graphene oxidepolyethyleneimine/p53 plasmid (Dox/GO-PEI/p53) were fabricated and employed to investigate the synergistic manner between Dox and p53 in the inhibition of HeLa cell growth. GO was conjugated with PEI to form the GO-PEI backbone as the delivery vector. The GO backbone provided surfaces with a high specific area to load Dox via the pi-pi stacking interaction, and was able to release Dox significantly faster at pH 5.0 than at pH 7.0, while the positively charged PEI section of GO-PEI could condense plasmids into GO-PEI/DNA nanoparticles via the electrostatic interaction. The nanoparticles efficiently mediated the transfection of DNA in HeLa cells, with lower cytotoxicity compared to PEI/DNA nanoparticles. Furthermore, the complexes of Dox/GO-PEI/p53 released Dox and expressed p53 gene in a sequential manner, and showed successive inhibition of the in vitro growth of HeLa cells. This type of drug/GO-PEI/DNA complex can be employed as a platform to investigate the coordination pattern between chemical drugs and therapeutic genes for tumor therapy. (C) 2017 Published by Elsevier Ltd on behalf of The editorial office of Journal of Materials Science & Technology.; Co-delivery of chemical drugs and therapeutic genes for synergistic therapy provides a promising strategy to treat devastating diseases. However, the real-time coordination patterns between chemical drugs and therapeutic genes remain poorly understood. Herein, the complexes of doxorubicin/graphene oxidepolyethyleneimine/p53 plasmid (Dox/GO-PEI/p53) were fabricated and employed to investigate the synergistic manner between Dox and p53 in the inhibition of HeLa cell growth. GO was conjugated with PEI to form the GO-PEI backbone as the delivery vector. The GO backbone provided surfaces with a high specific area to load Dox via the pi-pi stacking interaction, and was able to release Dox significantly faster at pH 5.0 than at pH 7.0, while the positively charged PEI section of GO-PEI could condense plasmids into GO-PEI/DNA nanoparticles via the electrostatic interaction. The nanoparticles efficiently mediated the transfection of DNA in HeLa cells, with lower cytotoxicity compared to PEI/DNA nanoparticles. Furthermore, the complexes of Dox/GO-PEI/p53 released Dox and expressed p53 gene in a sequential manner, and showed successive inhibition of the in vitro growth of HeLa cells. This type of drug/GO-PEI/DNA complex can be employed as a platform to investigate the coordination pattern between chemical drugs and therapeutic genes for tumor therapy. (C) 2017 Published by Elsevier Ltd on behalf of The editorial office of Journal of Materials Science & Technology.
部门归属[xie, bei ; yi, jipeng ; peng, jian ; zhao, dapeng ; nie, hemin] hunan univ, inst bionanotechnol & tissue engn, coll life sci, changsha 410082, hunan, peoples r china ; [zhang, xing] chinese acad sci, shenyang natl lab mat sci, inst met res, shenyang 110016, liaoning, peoples r china ; [lei, lei] cent s univ, xiangya stomatol hosp, dept orthodont, changsha 410008, hunan, peoples r china ; [lei, zhixin] changsha dmy med technol co ltd, changsha 410005, hunan, peoples r china ; [nie, hemin] hunan univ, shenzhen res inst, nanshan hi new technol & ind pk, shenzhen 518057, peoples r china
关键词Graphene Oxide Polyethyleneimine Doxorubicin P53 Successive Inhibition
学科领域Materials Science, Multidisciplinary ; Metallurgy & Metallurgical Engineering
资助者National Natural Science Foundation of China [31670997]; Natural Science Foundation of Hunan Province [2015JJ1007]; Basic Research Program of Shenzhen City [JCYJ20160530193417959]
收录类别SCI
语种英语
文献类型期刊论文
条目标识符http://ir.imr.ac.cn/handle/321006/79160
专题中国科学院金属研究所
通讯作者Nie, HM (reprint author), Hunan Univ, Inst Bionanotechnol & Tissue Engn, Coll Life Sci, Changsha 410082, Hunan, Peoples R China.; Zhang, X (reprint author), Chinese Acad Sci, Shenyang Natl Lab Mat Sci, Inst Met Res, Shenyang 110016, Liaoning, Peoples R China.
推荐引用方式
GB/T 7714
Xie, Bei,Yi, Jipeng,Peng, Jian,et al. Characterization of synergistic anti-tumor effects of doxorubicin and p53 via graphene oxide-polyethyleneimine nanocarriers[J]. JOURNAL MATER SCI TECHNOL,2017,33(8):807-814.
APA Xie, Bei.,Yi, Jipeng.,Peng, Jian.,Zhang, Xing.,Lei, Lei.,...&Zhang, X .(2017).Characterization of synergistic anti-tumor effects of doxorubicin and p53 via graphene oxide-polyethyleneimine nanocarriers.JOURNAL MATER SCI TECHNOL,33(8),807-814.
MLA Xie, Bei,et al."Characterization of synergistic anti-tumor effects of doxorubicin and p53 via graphene oxide-polyethyleneimine nanocarriers".JOURNAL MATER SCI TECHNOL 33.8(2017):807-814.
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