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2D transition metal carbide MXene as a robust biosensing platform for enzyme immobilization and ultrasensitive detection of phenol
Wu, LX; Lu, XB; Dhanjai; Wu, ZS; Dong, YF; Wang, XH; Zheng, SH; Chen, JP; Lu, XB (reprint author), Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China.
2018-06-01
发表期刊BIOSENSORS & BIOELECTRONICS
ISSN0956-5663
卷号107页码:69-75
摘要MXene-Ti3C2, as a new class of two-dimensional (2D) transition metal carbides (or nitrides), has been synthesized by exfoliating pristine Ti3AlC2 phases with hydrofluoric acid. The SEM and XRD images show that the resultant MXene possesses a graphene-like 2D nanostructure. and the surface of MXene has been partially terminated with-OH, thus providing a favorable microenvironment for enzyme immobilization and retaining their bioactivity and stability. Considering the unique metallic conductivity, biocompatibility and good dispersion in aqueous phase, the as-prepared MXene was explored as a new matrix to immobilize tyrosinase (a model enzyme) for fabricating a mediator-free biosensor for ultrasensidve and rapid detection of phenol. The varying electrochemical measurements were used to investigate the electrochemical performance of MXene-based tyrosinase biosensors. The results revealed that the direct electron transfer between tyrosinase and electrode could be easily achieved via a surface-controlled electrochemical process. The fabricated MXene-based tyrosinase biosensors exhibited good analytical performance over a wide linear range from 0.05 to 15.5 mu mol L-1, with a low detection limit of 12 nmol L-1 and a sensitivity of 414.4 mA M-1. The proposed biosensing approach also demonstrated good repeatability, reproducibility, long-term stability and high recovery for phenol detection in real water samples. With those excellent performances, MXene with graphene-like structure is proved to be a robust and versatile electrochemical biosensing platform for enzyme-based biosensors and biocatalysis, and has wide potential applications in biomedical detection and environmental analysis.; MXene-Ti3C2, as a new class of two-dimensional (2D) transition metal carbides (or nitrides), has been synthesized by exfoliating pristine Ti3AlC2 phases with hydrofluoric acid. The SEM and XRD images show that the resultant MXene possesses a graphene-like 2D nanostructure. and the surface of MXene has been partially terminated with-OH, thus providing a favorable microenvironment for enzyme immobilization and retaining their bioactivity and stability. Considering the unique metallic conductivity, biocompatibility and good dispersion in aqueous phase, the as-prepared MXene was explored as a new matrix to immobilize tyrosinase (a model enzyme) for fabricating a mediator-free biosensor for ultrasensidve and rapid detection of phenol. The varying electrochemical measurements were used to investigate the electrochemical performance of MXene-based tyrosinase biosensors. The results revealed that the direct electron transfer between tyrosinase and electrode could be easily achieved via a surface-controlled electrochemical process. The fabricated MXene-based tyrosinase biosensors exhibited good analytical performance over a wide linear range from 0.05 to 15.5 mu mol L-1, with a low detection limit of 12 nmol L-1 and a sensitivity of 414.4 mA M-1. The proposed biosensing approach also demonstrated good repeatability, reproducibility, long-term stability and high recovery for phenol detection in real water samples. With those excellent performances, MXene with graphene-like structure is proved to be a robust and versatile electrochemical biosensing platform for enzyme-based biosensors and biocatalysis, and has wide potential applications in biomedical detection and environmental analysis.
部门归属[wu, lingxia ; lu, xianbo ; dhanjai ; chen, jiping] chinese acad sci, dalian inst chem phys, cas key lab separat sci analyt chem, 457 zhongshan rd, dalian 116023, peoples r china ; [wu, zhong-shuai ; dong, yanfeng ; zheng, shuanghao] chinese acad sci, dalian inst chem phys, dalian natl lab clean energy, 457 zhongshan rd, dalian 116023, peoples r china ; [wu, lingxia ; zheng, shuanghao] univ chinese acad sci, beijing 100049, peoples r china ; [wang, xiaohui] chinese acad sci, inst met res, shenyang natl lab mat sci, shenyang 110016, liaoning, peoples r china
关键词2-dimensional Titanium Carbide Water-soluble Graphene Ti3c2 Mxene Functionalized Graphene Direct Electrochemistry Aqueous-media Ion Batteries Adsorption Intercalation Exfoliation
学科领域Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
资助者National Natural Science Foundation of China [21577139, 21475130, 51572259]; Special Fund for Agro-scientific Research in the Public Interest of China [201503108]; Exploratory Research Program of Shanxi Yanchang Petroleum (Group) CO., LTD [DICP ZZBS201708]; DICP [DICP ZZBS201708]
收录类别SCI
语种英语
文献类型期刊论文
条目标识符http://ir.imr.ac.cn/handle/321006/79299
专题中国科学院金属研究所
通讯作者Lu, XB (reprint author), Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China.
推荐引用方式
GB/T 7714
Wu, LX,Lu, XB,Dhanjai,et al. 2D transition metal carbide MXene as a robust biosensing platform for enzyme immobilization and ultrasensitive detection of phenol[J]. BIOSENSORS & BIOELECTRONICS,2018,107:69-75.
APA Wu, LX.,Lu, XB.,Dhanjai.,Wu, ZS.,Dong, YF.,...&Lu, XB .(2018).2D transition metal carbide MXene as a robust biosensing platform for enzyme immobilization and ultrasensitive detection of phenol.BIOSENSORS & BIOELECTRONICS,107,69-75.
MLA Wu, LX,et al."2D transition metal carbide MXene as a robust biosensing platform for enzyme immobilization and ultrasensitive detection of phenol".BIOSENSORS & BIOELECTRONICS 107(2018):69-75.
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