In Vitro and in Vivo Studies on Biomedical Magnesium Low-Alloying with Elements Gadolinium and Zinc for Orthopedic Implant Applications

IMR OpenIR

	In Vitro and in Vivo Studies on Biomedical Magnesium Low-Alloying with Elements Gadolinium and Zinc for Orthopedic Implant Applications
	Bian, D; Deng, JX; Li, N; Chu, X; Liu, Y; Li, WT; Cai, H; Xiu, P; Zhang, Y; Guan, ZP; Zheng, YF; Kou, YH; Jiang, BG; Chen, RS; Zheng, YF (reprint author), Peking Univ, Coll Engn, Dept Mat Sci & Engn, Beijing 100871, Peoples R China.; Jiang, BG (reprint author), Peking Univ, Peoples Hosp, Dept Trauma & Orthoped, Beijing 100044, Peoples R China.; Chen, RS (reprint author), Chinese Acad Sci, Inst Met Res, Grp Magnesium Alloys & Their Applicat, Shenyang 110016, Liaoning, Peoples R China.
	2018-02-07
发表期刊	ACS APPLIED MATERIALS & INTERFACES
ISSN	1944-8244
卷号	10 期号:5 页码:4394-4408
摘要	Ternary magnesium alloys with low combined addition of elements gadolinium and zinc were developed in the present work, with their microstructures, mechanical properties, in vitro degradation behaviors, and cytotoxicity being systematically studied. Furthermore, the Mg-1.8Zn-0.2Gd alloy, with the best in vitro performance, was implanted into Sprague Dawley rats to examine its in vivo degradation performance for up to 6 months. It was found that Mg-1.8Zn-0.2Gd, composed of a single alpha-Mg phase, owned excellent strength and toughness that were comparable to the CE marked MAGNEZIX, the mischmetal added Mg alloy. Owing to the uniform single-phased microstructure, the degradation rate of this alloy was around 0.12 mm/y measured by electrochemical testing; which was comparable to high purity magnesium. Moreover, the Mg-1.8Zn-0.2Gd alloy exhibited no cytotoxicity to L929, MG63, and VSMC cells. In vivo degradation characterized by micro computed tomography revealed that the Mg-1.8Zn-0.2Gd implant could maintain structural integrity in the first 2 months, and serious degradation could be observed after 6 months. A remarkable 100% survival rate of experimental animals was observed with no negative effects on bone tissues. The implant and the surrounding bone were well integrated within 2 months, implying good biocompatibility and osteoconductivity of the experimental alloy. On the basis of the above findings, the feasibility of Mg Zn-Gd alloys for use as orthopedic implants was systematically discussed. This study provides a new strategy for development of high-performance Mg-rare earth (RE)-based alloys with superior mechanical properties and corrosion resistance while effectively avoiding the possible standing toxic effect of RE elements.; Ternary magnesium alloys with low combined addition of elements gadolinium and zinc were developed in the present work, with their microstructures, mechanical properties, in vitro degradation behaviors, and cytotoxicity being systematically studied. Furthermore, the Mg-1.8Zn-0.2Gd alloy, with the best in vitro performance, was implanted into Sprague Dawley rats to examine its in vivo degradation performance for up to 6 months. It was found that Mg-1.8Zn-0.2Gd, composed of a single alpha-Mg phase, owned excellent strength and toughness that were comparable to the CE marked MAGNEZIX, the mischmetal added Mg alloy. Owing to the uniform single-phased microstructure, the degradation rate of this alloy was around 0.12 mm/y measured by electrochemical testing; which was comparable to high purity magnesium. Moreover, the Mg-1.8Zn-0.2Gd alloy exhibited no cytotoxicity to L929, MG63, and VSMC cells. In vivo degradation characterized by micro computed tomography revealed that the Mg-1.8Zn-0.2Gd implant could maintain structural integrity in the first 2 months, and serious degradation could be observed after 6 months. A remarkable 100% survival rate of experimental animals was observed with no negative effects on bone tissues. The implant and the surrounding bone were well integrated within 2 months, implying good biocompatibility and osteoconductivity of the experimental alloy. On the basis of the above findings, the feasibility of Mg Zn-Gd alloys for use as orthopedic implants was systematically discussed. This study provides a new strategy for development of high-performance Mg-rare earth (RE)-based alloys with superior mechanical properties and corrosion resistance while effectively avoiding the possible standing toxic effect of RE elements.
部门归属	[bian, dong ; li, nan ; liu, yang ; li, wenting ; zheng, yufeng] peking univ, coll engn, dept mat sci & engn, beijing 100871, peoples r china ; [deng, jiuxu ; kou, yuhui ; jiang, baoguo] peking univ, peoples hosp, dept trauma & orthoped, beijing 100044, peoples r china ; [guan, zhenpeng] peking univ, peoples hosp, arthrit clin & res ctr, beijing 100044, peoples r china ; [chu, xiao ; zhang, yu] guangzhou gen hosp guangzhou mil command, guangdong key lab orthopaed technol & implant mat, dept orthoped, guangzhou 510010, guangdong, peoples r china ; [cai, hong ; xiu, peng] peking univ, hosp 3, dept orthoped, beijing 100191, peoples r china ; [chen, rongshi] chinese acad sci, inst met res, grp magnesium alloys & their applicat, shenyang 110016, liaoning, peoples r china
关键词	Simulated Body-fluid Zn-gd Alloys Mg-zn Mechanical-properties Corrosion Behavior Rare-earth Pure Magnesium Rabbit Model Binary Mg Bone
学科领域	Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
资助者	National Key Research and Development Program of China [2016YFC1102402]; National Natural Science Foundation of China [51431002]; NSFC/RGC Joint Research Scheme [51361165101, 5161101031]; NSFC-RFBR Cooperation [51611130054]
收录类别	SCI
语种	英语
WOS记录号	WOS:000424851600006
引用统计	被引频次：75[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/79517
专题	中国科学院金属研究所
通讯作者	Zheng, YF (reprint author), Peking Univ, Coll Engn, Dept Mat Sci & Engn, Beijing 100871, Peoples R China.; Jiang, BG (reprint author), Peking Univ, Peoples Hosp, Dept Trauma & Orthoped, Beijing 100044, Peoples R China.; Chen, RS (reprint author), Chinese Acad Sci, Inst Met Res, Grp Magnesium Alloys & Their Applicat, Shenyang 110016, Liaoning, Peoples R China.
推荐引用方式 GB/T 7714	Bian, D,Deng, JX,Li, N,et al. In Vitro and in Vivo Studies on Biomedical Magnesium Low-Alloying with Elements Gadolinium and Zinc for Orthopedic Implant Applications[J]. ACS APPLIED MATERIALS & INTERFACES,2018,10(5):4394-4408.
APA	Bian, D.,Deng, JX.,Li, N.,Chu, X.,Liu, Y.,...&Chen, RS .(2018).In Vitro and in Vivo Studies on Biomedical Magnesium Low-Alloying with Elements Gadolinium and Zinc for Orthopedic Implant Applications.ACS APPLIED MATERIALS & INTERFACES,10(5),4394-4408.
MLA	Bian, D,et al."In Vitro and in Vivo Studies on Biomedical Magnesium Low-Alloying with Elements Gadolinium and Zinc for Orthopedic Implant Applications".ACS APPLIED MATERIALS & INTERFACES 10.5(2018):4394-4408.