IMR OpenIR
TGF-beta 1 promotes the osteoinduction of human osteoblasts via the PI3K/AKT/mTOR/S6K1 signalling pathway
Zhang, Zhaodong; Zhang, Xiuzhi; Zhao, Dewei; Liu, Baoyi; Wang, Benjie; Yu, Weiting; Li, Junlei; Yu, Xiaobing; Cao, Fang; Zheng, Guoshuang; Zhang, Yao; Liu, Yupeng
2019-05-01
Source PublicationMOLECULAR MEDICINE REPORTS
ISSN1791-2997
Volume19Issue:5Pages:3505-3518
AbstractTransforming growth factor 1 (TGF-1) has been suggested to be a candidate cytokine in the field of bone tissue engineering. Cytokines serve important roles in tissue engineering, particularly in the repair of bone damage; however, the underlying molecular mechanisms remain unclear. In the present study, the effects of TGF-1 on the osteogenesis and motility of hFOB1.19 human osteoblasts were demonstrated via the phenotype and gene expression of cells. Additionally, the role of the phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin/S6 kinase 1 (PI3K/AKT/mTOR/S6K1) signalling pathway in the effects of TGF-1 on osteoblasts was investigated. It was demonstrated using Cell Counting Kit-8 and flow cytometry assays that the proliferation of human osteoblasts was promoted by 1 ng/ml TGF-1. In addition, alkaline phosphatase activity, Alizarin red staining, scratch-wound and Transwell assays were conducted. It was revealed that osteogenesis and the migration of cells were regulated by TGF-1 via the upregulation of osteogenic and migration-associated genes. Alterations in the expression of osteogenesis- and migration-associated genes were evaluated following pre-treatment with a PI3K/AKT inhibitor (LY294002) and an mTOR/S6K1 inhibitor (rapamycin), with or without TGF-1. The results indicated that TGF-1 affected the osteogenesis and mineralisation of osteoblasts via the PI3K/AKT signalling pathway. Furthermore, TGF-1 exhibited effects on mTOR/S6K1 downstream of PI3K/AKT. The present study demonstrated that TGF-1 promoted the proliferation, differentiation and migration of human hFOB1.19 osteoblasts, and revealed that TGF-1 affected the biological activity of osteoblasts via the PI3K/AKT/mTOR/S6K1 signalling pathway. Our findings may provide novel insight to aid the development of bone tissue engineering methods for the treatment of bone injury.
Keywordtransforming growth factor 1 osteoinduction phosphatidylinositol 3-kinase protein kinase B mammalian target of rapamycin S6 kinase 1
Indexed BySCI
Language英语
WOS IDWOS:000465868800015
PublisherSPANDIDOS PUBL LTD
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/81131
Collection中国科学院金属研究所
Recommended Citation
GB/T 7714
Zhang, Zhaodong,Zhang, Xiuzhi,Zhao, Dewei,et al. TGF-beta 1 promotes the osteoinduction of human osteoblasts via the PI3K/AKT/mTOR/S6K1 signalling pathway[J]. MOLECULAR MEDICINE REPORTS,2019,19(5):3505-3518.
APA Zhang, Zhaodong.,Zhang, Xiuzhi.,Zhao, Dewei.,Liu, Baoyi.,Wang, Benjie.,...&Liu, Yupeng.(2019).TGF-beta 1 promotes the osteoinduction of human osteoblasts via the PI3K/AKT/mTOR/S6K1 signalling pathway.MOLECULAR MEDICINE REPORTS,19(5),3505-3518.
MLA Zhang, Zhaodong,et al."TGF-beta 1 promotes the osteoinduction of human osteoblasts via the PI3K/AKT/mTOR/S6K1 signalling pathway".MOLECULAR MEDICINE REPORTS 19.5(2019):3505-3518.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Zhaodong]'s Articles
[Zhang, Xiuzhi]'s Articles
[Zhao, Dewei]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Zhaodong]'s Articles
[Zhang, Xiuzhi]'s Articles
[Zhao, Dewei]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Zhaodong]'s Articles
[Zhang, Xiuzhi]'s Articles
[Zhao, Dewei]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.