IMR OpenIR
Effect of Microarc Oxidation-Treated Ti6Al4V Scaffold Following Low-Intensity Pulsed Ultrasound Stimulation on Osteogenic Cells in Vitro
Chen, Jie; Li, Jiongjiong; Hu, Fu; Zou, Qin; Mei, Quanjing; Li, Shujun; Hao, Yulin; Hou, Wentao; Li, Jidong; Li, Yubao; Zuo, Yi
2019-02-01
Source PublicationACS BIOMATERIALS SCIENCE & ENGINEERING
ISSN2373-9878
Volume5Issue:2Pages:572-581
AbstractThe porous Ti6Al4V alloy has emerged to solve the biomechanical mismatch between implant and bone as its tunable mechanical properties. Cell-surface interaction is related to numerous factors-the surface's chemical composition, morphological structure, and external effect. The microarc oxidation (MAO) method was employed in this study to improve the surface properties of scaffolds produced by Electron Beam Melting (EBM), and low-intensity pulse ultrasound (LIPUS) provides physical stimulation for cells in vitro. Although MAO-treated and untreated scaffolds shared the same three-dimensional (3D) structures, the former recreated a more affinity surface than the latter in the 3D room, which could stimulate cell adhesion, proliferation, and differentiation. Therefore, MG63 cells were represented with a stereoscopic cytoskeleton structure on the MAO-treated scaffold as numerous cellular filopodia/lamellipodia with rich extracellular matrix secretion, while flat and sheetlike cells were observed on the untreated scaffold. The expression of ALP, OCN, BMP2, Bmpr1a, and Runx2 were up-regulated by the MAO-treated scaffold; in addition, LIPUS stimulation effectively promoted cell proliferation and osteogenesis differentiation. In the future, the EBM-MAO strategy can be applied to prepare 3D hierarchical macro-/microstructure titanium implants for bone grafts, and LIPUS stimulation can be used as a therapeutic method simultaneously.
Keywordtitanium alloy scaffold EBM-MAO strategy affinity surface stereoscopic cytoskeleton structure LIPUS stimulation cell mineralization
Indexed BySCI
Language英语
WOS IDWOS:000458937900019
PublisherAMER CHEMICAL SOC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/81401
Collection中国科学院金属研究所
Recommended Citation
GB/T 7714
Chen, Jie,Li, Jiongjiong,Hu, Fu,et al. Effect of Microarc Oxidation-Treated Ti6Al4V Scaffold Following Low-Intensity Pulsed Ultrasound Stimulation on Osteogenic Cells in Vitro[J]. ACS BIOMATERIALS SCIENCE & ENGINEERING,2019,5(2):572-581.
APA Chen, Jie.,Li, Jiongjiong.,Hu, Fu.,Zou, Qin.,Mei, Quanjing.,...&Zuo, Yi.(2019).Effect of Microarc Oxidation-Treated Ti6Al4V Scaffold Following Low-Intensity Pulsed Ultrasound Stimulation on Osteogenic Cells in Vitro.ACS BIOMATERIALS SCIENCE & ENGINEERING,5(2),572-581.
MLA Chen, Jie,et al."Effect of Microarc Oxidation-Treated Ti6Al4V Scaffold Following Low-Intensity Pulsed Ultrasound Stimulation on Osteogenic Cells in Vitro".ACS BIOMATERIALS SCIENCE & ENGINEERING 5.2(2019):572-581.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Chen, Jie]'s Articles
[Li, Jiongjiong]'s Articles
[Hu, Fu]'s Articles
Baidu academic
Similar articles in Baidu academic
[Chen, Jie]'s Articles
[Li, Jiongjiong]'s Articles
[Hu, Fu]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Chen, Jie]'s Articles
[Li, Jiongjiong]'s Articles
[Hu, Fu]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.