IMR OpenIR
Regulation of osteogenesis and osteoclastogenesis by zoledronic acid loaded on biodegradable magnesium-strontium alloy
Li, Mei; Wan, Peng; Wang, Weidan; Yang, Ke; Zhang, Yu; Han, Yong
2019-01-30
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume9
AbstractInhibiting osteoclasts and osteoclast precursors to reduce bone resorption is an important strategy to treat osteoclast-related diseases, such as peri-prosthetic osteolysis. In this study, our objective was to study the role of zoledronic acid (ZA), as a highly potent and nitrogen-containing bisphosphonate, in promoting osteogenesis and inhibiting osteoclastogenesis properties of magnesium (Mg)-based implants. ZA was chemically associated with calcium phosphate (CaP) deposited on magnesiumstrontium (Mg-Sr) alloy, which was confirmed by the morphological observation, phase composition and drug releasing via SEM, XRD spectrum and High Performance Liquid Chromatography (HPLC), respectively. The in vitro performances indicated that ZA-CaP bilayer coating Mg-Sr alloy could enhance the proliferation and the osteogenic differentiation as well as the mineralization of pre-osteoblasts, however, induce the apoptosis and inhibit the osteoclast differentiation. We further investigated the possible molecular mechanisms by using Quantitative real-time PCR (qRT-PCR) and Western Blotting, and the results showed that ZA-CaP bilayer coating Mg-Sr alloy could regulate the osteogenesis and osteoclastogenesis through the Estrogen Receptor alpha (ER alpha) and NF-kappa B signaling pathway. Moreover, ZA-CaP bilayer coating Mg-Sr alloy could regulate the cross talk of osteoblast-osteoclast and increase the ratio of OPG: RANKL in the co-culture system through OPG/RANKL/RANK signaling pathway, which promoting the balance of bone remodeling process. Therefore, these promising results suggest the potential clinical applications of ZA pretreated Mg-Sr alloys for bone defect repairs and periprosthetical osteolysis due to the excessive differentitation and maturation of osteoclasts.
Indexed BySCI
Language英语
WOS IDWOS:000457128700010
PublisherNATURE PUBLISHING GROUP
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/81430
Collection中国科学院金属研究所
Recommended Citation
GB/T 7714
Li, Mei,Wan, Peng,Wang, Weidan,et al. Regulation of osteogenesis and osteoclastogenesis by zoledronic acid loaded on biodegradable magnesium-strontium alloy[J]. SCIENTIFIC REPORTS,2019,9.
APA Li, Mei,Wan, Peng,Wang, Weidan,Yang, Ke,Zhang, Yu,&Han, Yong.(2019).Regulation of osteogenesis and osteoclastogenesis by zoledronic acid loaded on biodegradable magnesium-strontium alloy.SCIENTIFIC REPORTS,9.
MLA Li, Mei,et al."Regulation of osteogenesis and osteoclastogenesis by zoledronic acid loaded on biodegradable magnesium-strontium alloy".SCIENTIFIC REPORTS 9(2019).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li, Mei]'s Articles
[Wan, Peng]'s Articles
[Wang, Weidan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li, Mei]'s Articles
[Wan, Peng]'s Articles
[Wang, Weidan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li, Mei]'s Articles
[Wan, Peng]'s Articles
[Wang, Weidan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.