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A Comparison of 1-and 3.2-MHz Low-Intensity Pulsed Ultrasound on Osteogenesis on Porous Titanium Alloy Scaffolds: An In Vitro and In Vivo Study
Feng, Lifang; Liu, Xiaohan; Cao, Hongjuan; Qin, Limei; Hou, Wentao; Wu, Lin
2019
Source PublicationJOURNAL OF ULTRASOUND IN MEDICINE
ISSN0278-4297
Volume38Issue:1Pages:191-202
AbstractObjectives Low-intensity pulsed ultrasound (LIPUS) combined with porous scaffolds can be used as a new therapy to treat bone defect repair. The aim of this study was to evaluate the effects of 1 and 3.2 MHz LIPUS on osteogenesis on porous Ti64 alloy scaffolds for both in vitro and in vivo studies. Methods Scaffolds were randomly divided into the high-frequency ultrasound group, low-frequency ultrasound group, and control group. Mouse pre-osteoblast cells were cultured with porous Ti-6Al-4V scaffolds in vitro to evaluate cell proliferation and differentiation. In addition, scaffolds were implanted into rabbit mandibular defects in vivo. The effects of LIPUS on bone regeneration were evaluated by observing the micro-computed tomography (micro-CT), toluidine blue staining, and von Kossa staining. Results The results revealed no significant difference in the cell counting kit-8 values between the ultrasound groups and control groups (P > .05). Compared with the control group, ultrasound promoted alkaline phosphatase activity and osteocalcin levels of the cells on the scaffolds (P < .05), but there was no significant difference between the two frequencies. In addition, histomorphologic analyses revealed that the volume and amount of new bone formation increased and that bone maturity improved in the ultrasound groups compared with the control group, but no significant difference was noted between the two frequencies. Conclusions Under the present experimental conditions, LIPUS promoted osteoblast differentiation and promoted bone maturity on porous Ti64 scaffolds. No significant differences were noted between the two frequencies.
Keywordbone formation frequency low-intensity pulsed ultrasound osteogenesis porous Ti64 scaffolds
Indexed BySCI
Language英语
WOS IDWOS:000453869000022
PublisherWILEY
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/81503
Collection中国科学院金属研究所
Recommended Citation
GB/T 7714
Feng, Lifang,Liu, Xiaohan,Cao, Hongjuan,et al. A Comparison of 1-and 3.2-MHz Low-Intensity Pulsed Ultrasound on Osteogenesis on Porous Titanium Alloy Scaffolds: An In Vitro and In Vivo Study[J]. JOURNAL OF ULTRASOUND IN MEDICINE,2019,38(1):191-202.
APA Feng, Lifang,Liu, Xiaohan,Cao, Hongjuan,Qin, Limei,Hou, Wentao,&Wu, Lin.(2019).A Comparison of 1-and 3.2-MHz Low-Intensity Pulsed Ultrasound on Osteogenesis on Porous Titanium Alloy Scaffolds: An In Vitro and In Vivo Study.JOURNAL OF ULTRASOUND IN MEDICINE,38(1),191-202.
MLA Feng, Lifang,et al."A Comparison of 1-and 3.2-MHz Low-Intensity Pulsed Ultrasound on Osteogenesis on Porous Titanium Alloy Scaffolds: An In Vitro and In Vivo Study".JOURNAL OF ULTRASOUND IN MEDICINE 38.1(2019):191-202.
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