AURKB and MAPK involvement in the regulation of the early stages of mouse zygote development

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	AURKB and MAPK involvement in the regulation of the early stages of mouse zygote development
其他题名	AURKB and MAPK involvement in the regulation of the early stages of mouse zygote development
	Xu Lin 1; Liu Tong 2; Han Feng 1; Zong ZhiHong 1; Wang GuoLi 1; Yu BingZhi 1; Zhang Jie 1
	2012
发表期刊	SCIENCE CHINA-LIFE SCIENCES
ISSN	1674-7305
卷号	55 期号:1 页码:47-56
摘要	Aurora kinases have become a hot topic for research as they have been found to play an important role in various stages of mitotic cell division and to participate in malignant conversions of tumors. The participation of Aurora kinases in the regulation of oocyte meiosis has been recently reported, but their participation in mammalian early embryonic development remained unclear. The object of our study was to establish the spatio-temporal expression pattern of Aurora kinase B (AURKB) in mouse zygotes during the first cleavage, to reveal its functions in the early development of mouse zygotes, and to define the involvement of AURKB in mitogen-activated protein kinase (MAPK) signaling. Our results showed that in mouse zygotes AURKB expression increased in G1 phase and peaked in M phase. AURKB protein distribution was found to be in association with nuclei and distributed throughout the cytoplasm in a cell cycle-dependent manner. Functional disruption of AURKB resulted in abnormal division phenotypes or mitotic impairments. U0126, a specific mitogen-activated protein kinase kinase (MEK) inhibitor, caused significantly altered morphologies of early embryos together with a decrease in protein expression and kinase activity of AURKB. Our results indicated that the activity of AURKB was required for regulating multiple stages of mitotic progression in the early development of mouse zygotes and was correlated with the activation of the MAPK pathway.
其他摘要	Aurora kinases have become a hot topic for research as they have been found to play an important role in various stages of mitotic cell division and to participate in malignant conversions of tumors. The participation of Aurora kinases in the regulation of oocyte meiosis has been recently reported, but their participation in mammalian early embryonic development remained unclear. The object of our study was to establish the spatio-temporal expression pattern of Aurora kinase B (AURKB) in mouse zygotes during the first cleavage, to reveal its functions in the early development of mouse zygotes, and to define the involvement of AURKB in mitogen-activated protein kinase (MAPK) signaling. Our results showed that in mouse zygotes AURKB expression increased in G1 phase and peaked in M phase. AURKB protein distribution was found to be in association with nuclei and distributed throughout the cytoplasm in a cell cycle-dependent manner. Functional disruption of AURKB resulted in abnormal division phenotypes or mitotic impairments. U0126, a specific mitogen-activated protein kinase kinase (MEK) inhibitor, caused significantly altered morphologies of early embryos together with a decrease in protein expression and kinase activity of AURKB. Our results indicated that the activity of AURKB was required for regulating multiple stages of mitotic progression in the early development of mouse zygotes and was correlated with the activation of the MAPK pathway.
关键词	AURORA-KINASE-A MICROTUBULE ORGANIZATION MEIOTIC MATURATION SIGNALING PATHWAY OOCYTES MITOSIS SPINDLE PROTEIN ACTIVATION SUBSTRATE AURKB MAPK mouse zygote mitosis cell cycle regulation
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China]
CSCD记录号	CSCD:4723664
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/87148
专题	中国科学院金属研究所
作者单位	1.中国科学院地质与地球物理研究所 2.中国科学院金属研究所
推荐引用方式 GB/T 7714	Xu Lin,Liu Tong,Han Feng,et al. AURKB and MAPK involvement in the regulation of the early stages of mouse zygote development[J]. SCIENCE CHINA-LIFE SCIENCES,2012,55(1):47-56.
APA	Xu Lin.,Liu Tong.,Han Feng.,Zong ZhiHong.,Wang GuoLi.,...&Zhang Jie.(2012).AURKB and MAPK involvement in the regulation of the early stages of mouse zygote development.SCIENCE CHINA-LIFE SCIENCES,55(1),47-56.
MLA	Xu Lin,et al."AURKB and MAPK involvement in the regulation of the early stages of mouse zygote development".SCIENCE CHINA-LIFE SCIENCES 55.1(2012):47-56.