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Optimization of microencapsulated recombinant CHO cell growth, endostatin production, and stability of microcapsule in vivo
Zhang, Ying1,2; Wang, Wei1; Xie, Yubing1; Yu, Weiting1; Lv, Guojun1,2; Guo, Xin1; Xiong, Ying1; Ma, Xiaojun1
Corresponding AuthorMa, Xiaojun(maxj@dicp.ae.en)
2008
Source PublicationJOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
ISSN1552-4973
Volume84BIssue:1Pages:79-88
AbstractMicroencapsulation of recombinant cells secreting endostatin offers a promising approach to tumor gene therapy in which therapeutic protein is delivered in a sustainable and long-term fashion by encapsulated recombinant cells. However, the studies of cell growth and protein production in vivo are very limited. In this study, the effects of microencapsulation parameters on in vivo cell growth, endostatin production, and microcapsule stability after implantation in the peritoneal cavity of mice were for the first time investigated. Microcapsules with liquid core reached higher cell density and endostatin production at day 18 than microcapsules with solid core. There was no significant difference in stability whether the core of the microcapsulle was solid or liquid. Decrease in microcapsule size increased the stability of microcapsule. The microcapsules kept intact in the peritoneal cavity of mice after 36 days of implantation when the microcapsules size was 240 mu m in diameter, which gave rise to high endostatin production as well. The optimized microencapsulation conditions for in vivo implantation are liquid core and 240 mu m in diameter. This study provides useful information for antiangiogenic gene therapy to tumors using microencapsulated recombinant cells. (c) 2007 Wiley Periodicals, Inc.
Keywordmicroencapsulation recombinant cell cell growth endostatin production stability in vivo
Indexed BySCI
Language英语
WOS Research AreaEngineering ; Materials Science
WOS SubjectEngineering, Biomedical ; Materials Science, Biomaterials
WOS IDWOS:000251802900010
PublisherWILEY-LISS
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/93234
Collection中国科学院金属研究所
Corresponding AuthorMa, Xiaojun
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch Chinese Acad Sci, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Ying,Wang, Wei,Xie, Yubing,et al. Optimization of microencapsulated recombinant CHO cell growth, endostatin production, and stability of microcapsule in vivo[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS,2008,84B(1):79-88.
APA Zhang, Ying.,Wang, Wei.,Xie, Yubing.,Yu, Weiting.,Lv, Guojun.,...&Ma, Xiaojun.(2008).Optimization of microencapsulated recombinant CHO cell growth, endostatin production, and stability of microcapsule in vivo.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS,84B(1),79-88.
MLA Zhang, Ying,et al."Optimization of microencapsulated recombinant CHO cell growth, endostatin production, and stability of microcapsule in vivo".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 84B.1(2008):79-88.
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