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Effect of ionic liquids as additives on the separation of bases and amino acids in HPLC
Jiang, Qiong1; Qiu, Hongdeng2,3; Wang, Xusheng2; Liu, Xia2; Zhang, Shusheng1
Corresponding AuthorZhang, Shusheng(menghuanrou@163.com)
2008
Source PublicationJOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN1082-6076
Volume31Issue:10Pages:1448-1457
AbstractIn this paper, four bases, including cytosine, thymine, adenine, 6-chlorouracil and four amino acids, including L-histidine, L-tyrosine, L-phenylalanine, and DL-tryptophane, were separated with different types of ionic liquids as additives for the mobile phase in high-performance liquid chromatography. Four different ILs, including 1-ethyl-3-methylimidazolium tetrafluoroborate, 1-butyl-3-methylimidazolium tetrafluoroborate, 1-hexyl-3-methylimidazolium tetrafluoroborate, and 1-butyl-3-methylimidazolium chloride were used. The effects of the length of the alkyl group on the imidazolium ring and its counterion, the concentrations of the ionic liquids on the retention factor of the bases and amino acids are investigated. The results showed that the retention and resolution of the bases and amino acids were improved when ionic liquids were used as additives. The mechanism at the separation with ionic liquids as additive to mobile phase is discussed.
Keywordionic liquid additive base amino acid high-performance liquid chromatography
DOI10.1080/10826070802039424
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000255272900005
PublisherTAYLOR & FRANCIS INC
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/93259
Collection中国科学院金属研究所
Corresponding AuthorZhang, Shusheng
Affiliation1.Zhengzhou Univ, Dept Chem, Key Lab Chem Biol & Organ Chem Henan, Zhengzhou 450052, Peoples R China
2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China
3.Chinese Acad Sci, Grad Sch Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Jiang, Qiong,Qiu, Hongdeng,Wang, Xusheng,et al. Effect of ionic liquids as additives on the separation of bases and amino acids in HPLC[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2008,31(10):1448-1457.
APA Jiang, Qiong,Qiu, Hongdeng,Wang, Xusheng,Liu, Xia,&Zhang, Shusheng.(2008).Effect of ionic liquids as additives on the separation of bases and amino acids in HPLC.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,31(10),1448-1457.
MLA Jiang, Qiong,et al."Effect of ionic liquids as additives on the separation of bases and amino acids in HPLC".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 31.10(2008):1448-1457.
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