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Expression, purification and characterization of recombinant Jerdonitin, a P-II class snake venom metalloproteinase comprising metalloproteinase and disintegrin domains
Zhu, Lili1,2; Yuan, Cai1,2; Chen, Zhuo1,2; Wang, Wanyu2,3; Huang, Mingdong1,2
Corresponding AuthorHuang, Mingdong(mhuang@fjirsm.ac.cn)
2010-02-01
Source PublicationTOXICON
ISSN0041-0101
Volume55Issue:2-3Pages:375-380
AbstractJerdonitin is a P-II class snake venom metalloproteinase comprising metalloproteinase and disintegrin domains. In this study, we established a high-level expression system in Pichia pastoris and developed a purification strategy for the recombinant Jerdonitin. This recombinant Jerdonitin degraded fibrinogen at a level of activity comparable with its wild type. The effects of recombinant Jerdonitin on inhibiting ADP-induced human platelet aggregation were in a dose-dependent manner with an IC(50) of 248 nM. In addition, we reported here that Jerdonitin can significantly inhibit the growth of several cell lines, including human liver cancer cells (Bel7402), human leukemia cells (K562) and human gastric carcinoma cells (BGC823). This study offers recombinant Jerdonitin that will be valuable for further functional and structural studies of Jerdonitin. (C) 2009 Elsevier Ltd. All rights reserved.
KeywordJerdonitin Snake venom metalloproteinases Recombinant protein expression Metalloproteinase Disintegrin Fibrinolysis activity Platelet aggregation Tumor cells MTT colorimetric assay Pichia pastoris
Funding OrganizationCAS ; Fujian NSF ; NSFC ; MOST
DOI10.1016/j.toxicon.2009.08.016
Indexed BySCI
Language英语
Funding ProjectCAS[KSCX2YW-R-082] ; Fujian NSF[200910029] ; NSFC[30625011] ; NSFC[30811130467] ; NSFC[30770429] ; MOST[2007CB914304] ; MOST[2006AA02A313]
WOS Research AreaPharmacology & Pharmacy ; Toxicology
WOS SubjectPharmacology & Pharmacy ; Toxicology
WOS IDWOS:000274579100026
PublisherPERGAMON-ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/101135
Collection中国科学院金属研究所
Corresponding AuthorHuang, Mingdong
Affiliation1.Chinese Acad Sci, Fujian Inst Res Struct Matter, State Key Lab Struct Chem, Fuzhou 350002, Peoples R China
2.Chinese Acad Sci, Grad Sch Chinese Acad Sci, Beijing 10039, Peoples R China
3.Chinese Acad Sci, Kunming Inst Zool, Dept Anim Toxinol, Kunming 650223, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Lili,Yuan, Cai,Chen, Zhuo,et al. Expression, purification and characterization of recombinant Jerdonitin, a P-II class snake venom metalloproteinase comprising metalloproteinase and disintegrin domains[J]. TOXICON,2010,55(2-3):375-380.
APA Zhu, Lili,Yuan, Cai,Chen, Zhuo,Wang, Wanyu,&Huang, Mingdong.(2010).Expression, purification and characterization of recombinant Jerdonitin, a P-II class snake venom metalloproteinase comprising metalloproteinase and disintegrin domains.TOXICON,55(2-3),375-380.
MLA Zhu, Lili,et al."Expression, purification and characterization of recombinant Jerdonitin, a P-II class snake venom metalloproteinase comprising metalloproteinase and disintegrin domains".TOXICON 55.2-3(2010):375-380.
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