Expression, purification and characterization of recombinant Jerdonitin, a P-II class snake venom metalloproteinase comprising metalloproteinase and disintegrin domains

doi:10.1016/j.toxicon.2009.08.016

IMR OpenIR

	Expression, purification and characterization of recombinant Jerdonitin, a P-II class snake venom metalloproteinase comprising metalloproteinase and disintegrin domains
	Zhu, Lili 1,2; Yuan, Cai 1,2; Chen, Zhuo 1,2; Wang, Wanyu 2,3; Huang, Mingdong 1,2
通讯作者	Huang, Mingdong(mhuang@fjirsm.ac.cn)
	2010-02-01
发表期刊	TOXICON
ISSN	0041-0101
卷号	55 期号:2-3 页码:375-380
摘要	Jerdonitin is a P-II class snake venom metalloproteinase comprising metalloproteinase and disintegrin domains. In this study, we established a high-level expression system in Pichia pastoris and developed a purification strategy for the recombinant Jerdonitin. This recombinant Jerdonitin degraded fibrinogen at a level of activity comparable with its wild type. The effects of recombinant Jerdonitin on inhibiting ADP-induced human platelet aggregation were in a dose-dependent manner with an IC(50) of 248 nM. In addition, we reported here that Jerdonitin can significantly inhibit the growth of several cell lines, including human liver cancer cells (Bel7402), human leukemia cells (K562) and human gastric carcinoma cells (BGC823). This study offers recombinant Jerdonitin that will be valuable for further functional and structural studies of Jerdonitin. (C) 2009 Elsevier Ltd. All rights reserved.
关键词	Jerdonitin Snake venom metalloproteinases Recombinant protein expression Metalloproteinase Disintegrin Fibrinolysis activity Platelet aggregation Tumor cells MTT colorimetric assay Pichia pastoris
资助者	CAS ; Fujian NSF ; NSFC ; MOST
DOI	10.1016/j.toxicon.2009.08.016
收录类别	SCI
语种	英语
资助项目	CAS[KSCX2YW-R-082] ; Fujian NSF[200910029] ; NSFC[30625011] ; NSFC[30811130467] ; NSFC[30770429] ; MOST[2007CB914304] ; MOST[2006AA02A313]
WOS研究方向	Pharmacology & Pharmacy ; Toxicology
WOS类目	Pharmacology & Pharmacy ; Toxicology
WOS记录号	WOS:000274579100026
出版者	PERGAMON-ELSEVIER SCIENCE LTD
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/101135
专题	中国科学院金属研究所
通讯作者	Huang, Mingdong
作者单位	1.Chinese Acad Sci, Fujian Inst Res Struct Matter, State Key Lab Struct Chem, Fuzhou 350002, Peoples R China 2.Chinese Acad Sci, Grad Sch Chinese Acad Sci, Beijing 10039, Peoples R China 3.Chinese Acad Sci, Kunming Inst Zool, Dept Anim Toxinol, Kunming 650223, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Lili,Yuan, Cai,Chen, Zhuo,et al. Expression, purification and characterization of recombinant Jerdonitin, a P-II class snake venom metalloproteinase comprising metalloproteinase and disintegrin domains[J]. TOXICON,2010,55(2-3):375-380.
APA	Zhu, Lili,Yuan, Cai,Chen, Zhuo,Wang, Wanyu,&Huang, Mingdong.(2010).Expression, purification and characterization of recombinant Jerdonitin, a P-II class snake venom metalloproteinase comprising metalloproteinase and disintegrin domains.TOXICON,55(2-3),375-380.
MLA	Zhu, Lili,et al."Expression, purification and characterization of recombinant Jerdonitin, a P-II class snake venom metalloproteinase comprising metalloproteinase and disintegrin domains".TOXICON 55.2-3(2010):375-380.