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Effect of calcium on the uptake of glutamate by synaptosomes: possible involvement of two different mechanisms
Zhu, BG; Chen, YZ; Xing, BR
Corresponding AuthorZhu, BG()
1999
Source PublicationJOURNAL OF NEURAL TRANSMISSION
ISSN0300-9564
Volume106Issue:3-4Pages:257-264
AbstractThe effects of Ca2+ on glutamate uptake by synaptosomes were investigated. Glutamate uptake was decreased in the absence of Ca2+ with 2 mM EGTA added to the medium. After a 8-min preincubation, the uptake reduced to the greatest extent (about 64% of the uptake measured in Ca2+ medium). According to the kinetic analysis, lack of Ca2+ resulted in a reduction of the Vmax for glutamate uptake. These results suggested external Ca2+ was necessary for the optimal uptake of glutamate. TTX, an inhibitor of the voltage-dependent Na+ channel, almost reversed the reduction of glutamate uptake, indicating that the uptake might be impaired via the partial activation of the voltage-dependent tetrodotoxin-sensitive Na+ channel. On the other hand, glutamate uptake was decreased by trifluoperazine, a calmodulin antagonist, and KN-62, an inhibtor of Ca2+/calmodulin-dependent protein kinase II, implying that intrasynaptosomal Ca2+ might also have a role in the uptake of glutamte, and Ca2+/calmodulin-dependent processes, such as Ca2+/calmodulin-dependent protein kinase II, might be involved in the modulation of glutamate transporter activity.
Keywordglutamate uptake Ca2+ synaptosome Na+ channel calmodulin Na2+/calmodulin-dependent protein kinase II
Indexed BySCI
Language英语
WOS Research AreaNeurosciences & Neurology
WOS SubjectClinical Neurology ; Neurosciences
WOS IDWOS:000079984000004
PublisherSPRINGER-VERLAG WIEN
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/109869
Collection中国科学院金属研究所
Corresponding AuthorZhu, BG
Affiliation1.Second Mil Med Univ, Inst Neurosci, Dept Physiol, Shanghai 200433, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Physiol, Neurobiol Lab, Shanghai 200031, Peoples R China
Recommended Citation
GB/T 7714
Zhu, BG,Chen, YZ,Xing, BR. Effect of calcium on the uptake of glutamate by synaptosomes: possible involvement of two different mechanisms[J]. JOURNAL OF NEURAL TRANSMISSION,1999,106(3-4):257-264.
APA Zhu, BG,Chen, YZ,&Xing, BR.(1999).Effect of calcium on the uptake of glutamate by synaptosomes: possible involvement of two different mechanisms.JOURNAL OF NEURAL TRANSMISSION,106(3-4),257-264.
MLA Zhu, BG,et al."Effect of calcium on the uptake of glutamate by synaptosomes: possible involvement of two different mechanisms".JOURNAL OF NEURAL TRANSMISSION 106.3-4(1999):257-264.
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