Effect of calcium on the uptake of glutamate by synaptosomes: possible involvement of two different mechanisms

IMR OpenIR

	Effect of calcium on the uptake of glutamate by synaptosomes: possible involvement of two different mechanisms
	Zhu, BG ; Chen, YZ ; Xing, BR
通讯作者	Zhu, BG()
	1999
发表期刊	JOURNAL OF NEURAL TRANSMISSION
ISSN	0300-9564
卷号	106 期号:3-4 页码:257-264
摘要	The effects of Ca2+ on glutamate uptake by synaptosomes were investigated. Glutamate uptake was decreased in the absence of Ca2+ with 2 mM EGTA added to the medium. After a 8-min preincubation, the uptake reduced to the greatest extent (about 64% of the uptake measured in Ca2+ medium). According to the kinetic analysis, lack of Ca2+ resulted in a reduction of the Vmax for glutamate uptake. These results suggested external Ca2+ was necessary for the optimal uptake of glutamate. TTX, an inhibitor of the voltage-dependent Na+ channel, almost reversed the reduction of glutamate uptake, indicating that the uptake might be impaired via the partial activation of the voltage-dependent tetrodotoxin-sensitive Na+ channel. On the other hand, glutamate uptake was decreased by trifluoperazine, a calmodulin antagonist, and KN-62, an inhibtor of Ca2+/calmodulin-dependent protein kinase II, implying that intrasynaptosomal Ca2+ might also have a role in the uptake of glutamte, and Ca2+/calmodulin-dependent processes, such as Ca2+/calmodulin-dependent protein kinase II, might be involved in the modulation of glutamate transporter activity.
关键词	glutamate uptake Ca2+ synaptosome Na+ channel calmodulin Na2+/calmodulin-dependent protein kinase II
收录类别	SCI
语种	英语
WOS研究方向	Neurosciences & Neurology
WOS类目	Clinical Neurology ; Neurosciences
WOS记录号	WOS:000079984000004
出版者	SPRINGER-VERLAG WIEN
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/109869
专题	中国科学院金属研究所
通讯作者	Zhu, BG
作者单位	1.Second Mil Med Univ, Inst Neurosci, Dept Physiol, Shanghai 200433, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Physiol, Neurobiol Lab, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Zhu, BG,Chen, YZ,Xing, BR. Effect of calcium on the uptake of glutamate by synaptosomes: possible involvement of two different mechanisms[J]. JOURNAL OF NEURAL TRANSMISSION,1999,106(3-4):257-264.
APA	Zhu, BG,Chen, YZ,&Xing, BR.(1999).Effect of calcium on the uptake of glutamate by synaptosomes: possible involvement of two different mechanisms.JOURNAL OF NEURAL TRANSMISSION,106(3-4),257-264.
MLA	Zhu, BG,et al."Effect of calcium on the uptake of glutamate by synaptosomes: possible involvement of two different mechanisms".JOURNAL OF NEURAL TRANSMISSION 106.3-4(1999):257-264.