| Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome-wide transcriptomes | |
| Yang, M.1; Mu, T.1; Zhong, W.2; Olajuyin, A. M.2; Xing, J.1 | |
| Corresponding Author | Yang, M.(mhyang@ipe.ac.cn) |
| 2017-08-01 | |
| Source Publication | LETTERS IN APPLIED MICROBIOLOGY
 |
| ISSN | 0266-8254 |
| Volume | 65Issue:2Pages:165-172 |
| Abstract | For pyruvate-producing strains, intracellular reduced nicotinamide adenine dinucleotide (NADH) accumulation is the main reason for the glycolysis inhibition. Comparing with glucose, using sodium gluconate as carbon source brought a decrease in NADH production and an increase in pyruvate production in engineered strain YP211. In order to explore the metabolic advantages of gluconate, genome-wide transcriptome analysis was employed to compare the metabolic differences between the two carbon sources. The results showed that the transcription of the genes gntU, gntK, and gntT responsible for transport and phosphorylation of gluconate, and genes edd and eda belonging to the Entner-Doudoroff (ED) pathway, was significantly enhanced. This suggested that the shortest route for the synthesis of pyruvate from gluconate was activated, and the synthesis of NADH was halved. Besides, the transcription of genes glpABCDTKF related to the glycerol metabolism was significantly enhanced, which might be because glycerol metabolism pathways were activated in the absence of glucose. These results provided valuable information for the further design of metabolic pathways in the construction of pyruvate-producing strains. |
| Keyword | Entner-Doudoroff pathway genome-wide transcriptome gluconate metabolism pyruvate redox state |
| Funding Organization | National High Technology Research and Development Program of China |
| DOI | 10.1111/lam.12758 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National High Technology Research and Development Program of China[2014AA021900] |
| WOS Research Area | Biotechnology & Applied Microbiology ; Microbiology |
| WOS Subject | Biotechnology & Applied Microbiology ; Microbiology |
| WOS ID | WOS:000406935400009 |
| Publisher | WILEY |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/127005 |
| Collection | 中国科学院金属研究所 |
| Corresponding Author | Yang, M. |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing, Peoples R China 2.Grad Sch Chinese Acad Sci, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Yang, M.,Mu, T.,Zhong, W.,et al. Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome-wide transcriptomes[J]. LETTERS IN APPLIED MICROBIOLOGY,2017,65(2):165-172. |
| APA | Yang, M.,Mu, T.,Zhong, W.,Olajuyin, A. M.,&Xing, J..(2017).Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome-wide transcriptomes.LETTERS IN APPLIED MICROBIOLOGY,65(2),165-172. |
| MLA | Yang, M.,et al."Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome-wide transcriptomes".LETTERS IN APPLIED MICROBIOLOGY 65.2(2017):165-172. |
| Files in This Item: | There are no files associated with this item. | |||||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment