Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome-wide transcriptomes

doi:10.1111/lam.12758

IMR OpenIR

	Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome-wide transcriptomes
	Yang, M.1; Mu, T.1; Zhong, W.2; Olajuyin, A. M.2; Xing, J.1
通讯作者	Yang, M.(mhyang@ipe.ac.cn)
	2017-08-01
发表期刊	LETTERS IN APPLIED MICROBIOLOGY
ISSN	0266-8254
卷号	65 期号:2 页码:165-172
摘要	For pyruvate-producing strains, intracellular reduced nicotinamide adenine dinucleotide (NADH) accumulation is the main reason for the glycolysis inhibition. Comparing with glucose, using sodium gluconate as carbon source brought a decrease in NADH production and an increase in pyruvate production in engineered strain YP211. In order to explore the metabolic advantages of gluconate, genome-wide transcriptome analysis was employed to compare the metabolic differences between the two carbon sources. The results showed that the transcription of the genes gntU, gntK, and gntT responsible for transport and phosphorylation of gluconate, and genes edd and eda belonging to the Entner-Doudoroff (ED) pathway, was significantly enhanced. This suggested that the shortest route for the synthesis of pyruvate from gluconate was activated, and the synthesis of NADH was halved. Besides, the transcription of genes glpABCDTKF related to the glycerol metabolism was significantly enhanced, which might be because glycerol metabolism pathways were activated in the absence of glucose. These results provided valuable information for the further design of metabolic pathways in the construction of pyruvate-producing strains.
关键词	Entner-Doudoroff pathway genome-wide transcriptome gluconate metabolism pyruvate redox state
资助者	National High Technology Research and Development Program of China
DOI	10.1111/lam.12758
收录类别	SCI
语种	英语
资助项目	National High Technology Research and Development Program of China[2014AA021900]
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS记录号	WOS:000406935400009
出版者	WILEY
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/127005
专题	中国科学院金属研究所
通讯作者	Yang, M.
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing, Peoples R China 2.Grad Sch Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Yang, M.,Mu, T.,Zhong, W.,et al. Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome-wide transcriptomes[J]. LETTERS IN APPLIED MICROBIOLOGY,2017,65(2):165-172.
APA	Yang, M.,Mu, T.,Zhong, W.,Olajuyin, A. M.,&Xing, J..(2017).Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome-wide transcriptomes.LETTERS IN APPLIED MICROBIOLOGY,65(2),165-172.
MLA	Yang, M.,et al."Analysis of gluconate metabolism for pyruvate production in engineered Escherichia coli based on genome-wide transcriptomes".LETTERS IN APPLIED MICROBIOLOGY 65.2(2017):165-172.