| Purinyl-cobamide is a native prosthetic group of reductive dehalogenases | |
| Yan, Jun1,2,3,4,5; Bi, Meng1; Bourdon, Allen K.6; Farmer, Abigail T.6; Wang, Po-Hsiang7; Molenda, Olivia7; Quaile, Andrew T.7; Jiang, Nannan3,4,5,8; Yang, Yi3,9; Yin, Yongchao1; Simsir, Burcu3,9; Campagna, Shawn R.6; Edwards, Elizabeth A.7; Loffler, Frank E.1,3,4,5,8,9,10 | |
| Corresponding Author | Yan, Jun(junyan@iae.ac.cn) ; Loffler, Frank E.(frank.loeffler@utk.edu) |
| 2018 | |
| Source Publication | NATURE CHEMICAL BIOLOGY
 |
| ISSN | 1552-4450 |
| Volume | 14Issue:1Pages:8-+ |
| Abstract | Cobamides such as vitamin B-12 are structurally conserved, cobalt-containing tetrapyrrole biomolecules that have essential biochemical functions in all domains of life. In organohalide respiration, a vital biological process for the global cycling of natural and anthropogenic organohalogens, cobamides are the requisite prosthetic groups for carbon-halogen bond-cleaving reductive dehalogenases. This study reports the biosynthesis of a new cobamide with unsubstituted purine as the lower base and assigns unsubstituted purine a biological function by demonstrating that Co alpha-purinyl-cobamide (purinyl-Cba) is the native prosthetic group in catalytically active tetrachloroethene reductive dehalogenases of Desulfitobacterium hafniense. Cobamides featuring different lower bases are not functionally equivalent, and purinyl-Cba elicits different physiological responses in corrinoid-auxotrophic, organohalide-respiring bacteria. Given that cobamide-dependent enzymes catalyze key steps in essential metabolic pathways, the discovery of a novel cobamide structure and the realization that lower bases can effectively modulate enzyme activities generate opportunities to manipulate functionalities of microbiomes. |
| Funding Organization | Superfund Research Program under the National Institute of Environmental Health Sciences ; Strategic Environmental Research and Development Program (SERDP) ; Natural Science and Engineering Research Council of Canada (NSERC) Industrial Biocatalysis Network ; China-UT One-Hundred Scholars Program by the China Scholarship Council ; University of Tennessee |
| DOI | 10.1038/NCHEMBIO.2512 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Superfund Research Program under the National Institute of Environmental Health Sciences[R01ES024294] ; Strategic Environmental Research and Development Program (SERDP)[ER-2312] ; Natural Science and Engineering Research Council of Canada (NSERC) Industrial Biocatalysis Network ; China-UT One-Hundred Scholars Program by the China Scholarship Council ; University of Tennessee |
| WOS Research Area | Biochemistry & Molecular Biology |
| WOS Subject | Biochemistry & Molecular Biology |
| WOS ID | WOS:000419964200004 |
| Publisher | NATURE PUBLISHING GROUP |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/127238 |
| Collection | 中国科学院金属研究所 |
| Corresponding Author | Yan, Jun; Loffler, Frank E. |
| Affiliation | 1.Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA 2.Chinese Acad Sci, Inst Appl Ecol, Key Lab Pollut Ecol & Environm Engn, Shenyang, Liaoning, Peoples R China 3.Univ Tennessee, Ctr Environm Biotechnol, Knoxville, TN 37932 USA 4.Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN 37830 USA 5.Oak Ridge Natl Lab, Joint Inst Biol Sci, Oak Ridge, TN 37830 USA 6.Univ Tennessee, Dept Chem, Knoxville, TN 37996 USA 7.Univ Toronto, Dept Chem Engn & Appl Chem, Toronto, ON, Canada 8.Univ Tennessee, Bredesen Ctr Interdisciplinary Res & Grad Educ, Knoxville, TN 37996 USA 9.Univ Tennessee, Dept Civil & Environm Engn, Knoxville, TN 37996 USA 10.Univ Tennessee, Dept Biosyst Engn & Soil Sci, Knoxville, TN 37996 USA |
| Recommended Citation GB/T 7714 | Yan, Jun,Bi, Meng,Bourdon, Allen K.,et al. Purinyl-cobamide is a native prosthetic group of reductive dehalogenases[J]. NATURE CHEMICAL BIOLOGY,2018,14(1):8-+. |
| APA | Yan, Jun.,Bi, Meng.,Bourdon, Allen K..,Farmer, Abigail T..,Wang, Po-Hsiang.,...&Loffler, Frank E..(2018).Purinyl-cobamide is a native prosthetic group of reductive dehalogenases.NATURE CHEMICAL BIOLOGY,14(1),8-+. |
| MLA | Yan, Jun,et al."Purinyl-cobamide is a native prosthetic group of reductive dehalogenases".NATURE CHEMICAL BIOLOGY 14.1(2018):8-+. |
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