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Purinyl-cobamide is a native prosthetic group of reductive dehalogenases
Yan, Jun1,2,3,4,5; Bi, Meng1; Bourdon, Allen K.6; Farmer, Abigail T.6; Wang, Po-Hsiang7; Molenda, Olivia7; Quaile, Andrew T.7; Jiang, Nannan3,4,5,8; Yang, Yi3,9; Yin, Yongchao1; Simsir, Burcu3,9; Campagna, Shawn R.6; Edwards, Elizabeth A.7; Loffler, Frank E.1,3,4,5,8,9,10
Corresponding AuthorYan, Jun(junyan@iae.ac.cn) ; Loffler, Frank E.(frank.loeffler@utk.edu)
2018
Source PublicationNATURE CHEMICAL BIOLOGY
ISSN1552-4450
Volume14Issue:1Pages:8-+
AbstractCobamides such as vitamin B-12 are structurally conserved, cobalt-containing tetrapyrrole biomolecules that have essential biochemical functions in all domains of life. In organohalide respiration, a vital biological process for the global cycling of natural and anthropogenic organohalogens, cobamides are the requisite prosthetic groups for carbon-halogen bond-cleaving reductive dehalogenases. This study reports the biosynthesis of a new cobamide with unsubstituted purine as the lower base and assigns unsubstituted purine a biological function by demonstrating that Co alpha-purinyl-cobamide (purinyl-Cba) is the native prosthetic group in catalytically active tetrachloroethene reductive dehalogenases of Desulfitobacterium hafniense. Cobamides featuring different lower bases are not functionally equivalent, and purinyl-Cba elicits different physiological responses in corrinoid-auxotrophic, organohalide-respiring bacteria. Given that cobamide-dependent enzymes catalyze key steps in essential metabolic pathways, the discovery of a novel cobamide structure and the realization that lower bases can effectively modulate enzyme activities generate opportunities to manipulate functionalities of microbiomes.
Funding OrganizationSuperfund Research Program under the National Institute of Environmental Health Sciences ; Strategic Environmental Research and Development Program (SERDP) ; Natural Science and Engineering Research Council of Canada (NSERC) Industrial Biocatalysis Network ; China-UT One-Hundred Scholars Program by the China Scholarship Council ; University of Tennessee
DOI10.1038/NCHEMBIO.2512
Indexed BySCI
Language英语
Funding ProjectSuperfund Research Program under the National Institute of Environmental Health Sciences[R01ES024294] ; Strategic Environmental Research and Development Program (SERDP)[ER-2312] ; Natural Science and Engineering Research Council of Canada (NSERC) Industrial Biocatalysis Network ; China-UT One-Hundred Scholars Program by the China Scholarship Council ; University of Tennessee
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000419964200004
PublisherNATURE PUBLISHING GROUP
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/127238
Collection中国科学院金属研究所
Corresponding AuthorYan, Jun; Loffler, Frank E.
Affiliation1.Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA
2.Chinese Acad Sci, Inst Appl Ecol, Key Lab Pollut Ecol & Environm Engn, Shenyang, Liaoning, Peoples R China
3.Univ Tennessee, Ctr Environm Biotechnol, Knoxville, TN 37932 USA
4.Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN 37830 USA
5.Oak Ridge Natl Lab, Joint Inst Biol Sci, Oak Ridge, TN 37830 USA
6.Univ Tennessee, Dept Chem, Knoxville, TN 37996 USA
7.Univ Toronto, Dept Chem Engn & Appl Chem, Toronto, ON, Canada
8.Univ Tennessee, Bredesen Ctr Interdisciplinary Res & Grad Educ, Knoxville, TN 37996 USA
9.Univ Tennessee, Dept Civil & Environm Engn, Knoxville, TN 37996 USA
10.Univ Tennessee, Dept Biosyst Engn & Soil Sci, Knoxville, TN 37996 USA
Recommended Citation
GB/T 7714
Yan, Jun,Bi, Meng,Bourdon, Allen K.,et al. Purinyl-cobamide is a native prosthetic group of reductive dehalogenases[J]. NATURE CHEMICAL BIOLOGY,2018,14(1):8-+.
APA Yan, Jun.,Bi, Meng.,Bourdon, Allen K..,Farmer, Abigail T..,Wang, Po-Hsiang.,...&Loffler, Frank E..(2018).Purinyl-cobamide is a native prosthetic group of reductive dehalogenases.NATURE CHEMICAL BIOLOGY,14(1),8-+.
MLA Yan, Jun,et al."Purinyl-cobamide is a native prosthetic group of reductive dehalogenases".NATURE CHEMICAL BIOLOGY 14.1(2018):8-+.
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