Anti-fibrotic function of Cu-bearing stainless steel for reducing recurrence of urethral stricture after stent implantation

doi:10.1002/jbm.b.34005

IMR OpenIR

	Anti-fibrotic function of Cu-bearing stainless steel for reducing recurrence of urethral stricture after stent implantation
	Zhao, Jing; Ren, Ling; Liu, Meixia; Xi, Tong; Zhang, Bingchun; Yang, Ke
通讯作者	Ren, Ling(lren@imr.ac.cn) ; Yang, Ke(kyang@imr.ac.cn)
	2018-07-01
发表期刊	JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
ISSN	1552-4973
卷号	106 期号:5 页码:2019-2028
摘要	Recurrent stenosis is the main reason inducing the failure of urethral stricture treatment. Our previous study has found that the 316L type Cu bearing stainless steel (316L-Cu SS) showed antimicrobial activity and anti-encrustation performance when it was used for relieving urethral obstructer. However, whether it can reduce the occurrence of fibrosis or not, we need further investigation to compare the cellular and molecular responses of human urethral scar fibroblast cells (USFCs) on 316L-Cu SS and medical grade 316L stainless (316L SS, as a control). [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4- sulfophenyl)- 2H-tetrazolium (MTS) and Transwell were used to assess the cellular responses, which confirmed that 316L-Cu SS could inhibit proliferation and migration of USFCs. Molecular expressions of fibrosis were evaluated by western blot, real-time quantitative polymerase chain reaction (qPCR), and Cu/Zn superoxide dismutase (CuZnSOD) measurement. The results indicated that up-regulating of CuZnSOD attenuated the transforming growth factor-1 expression and phosphorylation of Smad3 after exposure to 316L-Cu SS. Besides, the content of collagen type I (COL1) and collagen type III (COL3) secreting into the culture medium measured by enzyme-linked immunosorbent assay were in accord with the results of messenger ribonucleic acids. Both of them exhibited lower levels of COL1/COL3 exposure to 316L-Cu SS, demonstrating the inhibitory performance of 316L-Cu SS against fibrosis. (c) 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2019-2028, 2018.
关键词	Cu bearing stainless steel fibrosis CuZnSOD TGF-beta 1 Smad3 COL1 COL3
资助者	National Basic Research Program of China ; National Natural Science Foundation of China ; Foundation of Jiangsu Collaborative Innovation Center of Biomedical Functional Materials
DOI	10.1002/jbm.b.34005
收录类别	SCI
语种	英语
资助项目	National Basic Research Program of China[2012CB619101] ; National Natural Science Foundation of China[51171186] ; Foundation of Jiangsu Collaborative Innovation Center of Biomedical Functional Materials
WOS研究方向	Engineering ; Materials Science
WOS类目	Engineering, Biomedical ; Materials Science, Biomaterials
WOS记录号	WOS:000435443500038
出版者	WILEY
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/128637
专题	中国科学院金属研究所
通讯作者	Ren, Ling; Yang, Ke
作者单位	Chinese Acad Sci, Inst Met Res, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Jing,Ren, Ling,Liu, Meixia,et al. Anti-fibrotic function of Cu-bearing stainless steel for reducing recurrence of urethral stricture after stent implantation[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS,2018,106(5):2019-2028.
APA	Zhao, Jing,Ren, Ling,Liu, Meixia,Xi, Tong,Zhang, Bingchun,&Yang, Ke.(2018).Anti-fibrotic function of Cu-bearing stainless steel for reducing recurrence of urethral stricture after stent implantation.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS,106(5),2019-2028.
MLA	Zhao, Jing,et al."Anti-fibrotic function of Cu-bearing stainless steel for reducing recurrence of urethral stricture after stent implantation".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 106.5(2018):2019-2028.