| Staphylococcal enterotoxin C2 stimulated the maturation of bone marrow derived dendritic cells via TLR-NF kappa B signaling pathway | |
| Yao, Songyuan1,2; Xu, Mingkai1; Li, Yansheng1,2; Zhou, Libao3; Liao, Hui3; Zhang, Huiwen1; Zhang, Chenggang1 | |
| Corresponding Author | Xu, Mingkai(mkxu@iae.ac.cn) |
| 2018-09-15 | |
| Source Publication | EXPERIMENTAL CELL RESEARCH
 |
| ISSN | 0014-4827 |
| Volume | 370Issue:2Pages:237-244 |
| Abstract | As a kind of superantigen, staphylococcal enterotoxin C2 (SEC2) is well known as a powerful immunomodulator. However, most previous studies about SEC2 focus on its T cell activating characters. But the direct effect of SEC2 on antigen-presenting cells (APCs) which are important for the T cell activation is not clearly. In this study, we investigated the effect of SEC2 on murine bone marrow-derived dendritic cells (BMDCs) which are known as the specialized professional APCs. Contrary to its effects on T cells, SEC2 could not induce proliferation or cytotoxicity to BMDCs even in high concentrations. While SEC2 could promote the mature of BMDCs with increased expression of co-stimulatory molecules on cell membrane such as CD80, CD86, and MHC II. The production of pro-inflammatory cytokines such as TNF-alpha, IFN-gamma and IL-6 were also increased in BMDCs treated with SEC2. We also found that SEC2 enhanced the genes expression of pattern recognition receptors including toll-like receptors 2 (TLR2) and TLR4 in BMDCs, and up-regulated the key signal molecule MyD88 in both mRNA and protein levels. In addition, SEC2 also caused I kappa B alpha degradating and NF kappa B p65 translocating from the cytoplasm to the nucleus in BMDCs. The siRNAs for both TLR2 and TLR4, as well as NFKB specific inhibitor BAY 11-7085 could inhibit the co-stimulatory molecules expression and pro-inflammatory cytokines releasing induced by SEC2. Moreover, TLR2/4 specific siRNAs inhibited p65 and MyD88 upregulation induced by SEC2. In summary, all our results indicated that SEC2 could stimulate BMDCs maturation through TLR-NFKB signaling pathways. |
| Keyword | Bone marrow-derived dendritic cells NF kappa B Staphylococcal enterotoxin C2 Toll like receptors |
| Funding Organization | Strategic Priority Research Program of the Chinese Academy of Sciences ; Science and Technology Plan Projects of Shenyang City |
| DOI | 10.1016/j.yexcr.2018.06.024 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12020225] ; Science and Technology Plan Projects of Shenyang City[Z17-7-013] ; Science and Technology Plan Projects of Shenyang City[Y17-4-003] |
| WOS Research Area | Oncology ; Cell Biology |
| WOS Subject | Oncology ; Cell Biology |
| WOS ID | WOS:000442979100006 |
| Publisher | ELSEVIER INC |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/129183 |
| Collection | 中国科学院金属研究所 |
| Corresponding Author | Xu, Mingkai |
| Affiliation | 1.Chinese Acad Sci, Inst Appl Ecol, Shenyang, Liaoning, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chengda Biotechnol Co Ltd, Shenyang, Liaoning, Peoples R China |
| Recommended Citation GB/T 7714 | Yao, Songyuan,Xu, Mingkai,Li, Yansheng,et al. Staphylococcal enterotoxin C2 stimulated the maturation of bone marrow derived dendritic cells via TLR-NF kappa B signaling pathway[J]. EXPERIMENTAL CELL RESEARCH,2018,370(2):237-244. |
| APA | Yao, Songyuan.,Xu, Mingkai.,Li, Yansheng.,Zhou, Libao.,Liao, Hui.,...&Zhang, Chenggang.(2018).Staphylococcal enterotoxin C2 stimulated the maturation of bone marrow derived dendritic cells via TLR-NF kappa B signaling pathway.EXPERIMENTAL CELL RESEARCH,370(2),237-244. |
| MLA | Yao, Songyuan,et al."Staphylococcal enterotoxin C2 stimulated the maturation of bone marrow derived dendritic cells via TLR-NF kappa B signaling pathway".EXPERIMENTAL CELL RESEARCH 370.2(2018):237-244. |
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