| Characterization and abolishment of the cyclopiazonic acids produced by Aspergillus oryzae HMP-F28 | |
| Cao, Ting1,2; Ling, Junhong3; Liu, Yi1,3; Chen, Xiaoqi1,2; Tian, Xiaoyue1,3; Meng, Dali3; Pan, Huaqi1; Hu, Jiangchun1; Wang, Nan1 | |
| Corresponding Author | Wang, Nan(wangn@iae.ac.cn) |
| 2018 | |
| Source Publication | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
 |
| ISSN | 0916-8451 |
| Volume | 82Issue:10Pages:1832-1839 |
| Abstract | Extracellular alkalinization and H2O2 production are important early events during induced resistance establishment in plants. In a screen for metabolites as plant resistance activators from 98 fungal isolates associated with marine sponge Hymeniacidon perleve, the cyclopiazonic acids (CPAs) produced by Aspergillus oryzae HMP-F28 induced significant extracellular alkalinization coupled with augmented H2O2 production in tobacco cell suspensions. Bioassay-guided fractionation led to the isolation and structural elucidation of a new CPA congener (4, 3-hydroxysperadine A) and three known ones (1-3). To construct a mutasynthetic strain to generate unnatural CPA analogues, a hybrid pks-nrps gene (cpaS) was disrupted to abolish the production of the critical precursor of cyclo-acetoacetyl-L-tryptophan (cAATrp) and all the downstream CPA products. Elimination of cAATrp will allow cAATrp mimics being processed by the CPA biosynthetic machinery to produce CPA derivatives with designed structural features. |
| Keyword | Cyclopiazonic acid Aspergillus oryzae oxidative burst hybrid pks-nrps plant immune |
| DOI | 10.1080/09168451.2018.1490170 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry ; Food Science & Technology |
| WOS Subject | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry, Applied ; Food Science & Technology |
| WOS ID | WOS:000445240200020 |
| Publisher | TAYLOR & FRANCIS LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/129434 |
| Collection | 中国科学院金属研究所 |
| Corresponding Author | Wang, Nan |
| Affiliation | 1.Chinese Acad Sci, Inst Appl Ecol, Shenyang, Liaoning, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 3.Shenyang Pharmaceut Univ, Sch Tradit Chinese Mat Med, Shenyang, Liaoning, Peoples R China |
| Recommended Citation GB/T 7714 | Cao, Ting,Ling, Junhong,Liu, Yi,et al. Characterization and abolishment of the cyclopiazonic acids produced by Aspergillus oryzae HMP-F28[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,2018,82(10):1832-1839. |
| APA | Cao, Ting.,Ling, Junhong.,Liu, Yi.,Chen, Xiaoqi.,Tian, Xiaoyue.,...&Wang, Nan.(2018).Characterization and abolishment of the cyclopiazonic acids produced by Aspergillus oryzae HMP-F28.BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,82(10),1832-1839. |
| MLA | Cao, Ting,et al."Characterization and abolishment of the cyclopiazonic acids produced by Aspergillus oryzae HMP-F28".BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 82.10(2018):1832-1839. |
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