Cu-bearing stainless steel reduces cytotoxicity and crystals adhesion after ureteral epithelial cells exposing to calcium oxalate monohydrate

doi:10.1038/s41598-018-32388-0

IMR OpenIR

	Cu-bearing stainless steel reduces cytotoxicity and crystals adhesion after ureteral epithelial cells exposing to calcium oxalate monohydrate
	Cao, Zhiqiang 1; Zhao, Jing 2; Yang, Ke 2
通讯作者	Cao, Zhiqiang(13309888392@163.com) ; Yang, Ke(kyang@imr.ac.cn)
	2018-09-20
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	8 页码:8
摘要	Calcium oxalate monohydrate (COM), which is the main component of encrustation, may result in cell membrane injury. In addition, cellular damage is suggested to be the primary event attributing to COM crystal binding. To study the interaction between cells and crystals after incubating with a Cu-bearing stainless steel (316L-Cu SS), MTS and flow cytometric analyses were used to assess the cellular responses. The results confirmed that 316L-Cu SS could inhibit cytotoxicity and cellular apoptosis of ureteral epithelial cells (UECs) after COM treatment. Furthermore, molecular expressions of Cu/ Zn superoxide dismutase (CuZnSOD), which were evaluated by western blot analysis and real-time quantitative PCR (qPCR), indicated that 316L-Cu SS could inhibit the oxidative stress attributing to up-regulating of CuZnSOD. Moreover, the crystal adhesion cytokine CD44 was examined with western blot and qPCR, and the corresponding hyaluronic (HA) secreted into the medium was measured by enzyme-linked immunosorbent assay (ELISA). All results were confirmed that the expressions of cells cultured with 316L-Cu SS were down-regulated, demonstrating the inhibitory performance of 316L-Cu SS against crystal adhesion.
资助者	National Natural Science Foundation of China ; General Financial Grant from China Postdoctoral Science Foundation
DOI	10.1038/s41598-018-32388-0
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[51631009] ; National Natural Science Foundation of China[51371168] ; General Financial Grant from China Postdoctoral Science Foundation[2015M582885]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000445079400015
出版者	NATURE PUBLISHING GROUP
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/129557
专题	中国科学院金属研究所
通讯作者	Cao, Zhiqiang; Yang, Ke
作者单位	1.Gen Hosp Shenyang Mil Reg, Shenyang 110840, Liaoning, Peoples R China 2.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Cao, Zhiqiang,Zhao, Jing,Yang, Ke. Cu-bearing stainless steel reduces cytotoxicity and crystals adhesion after ureteral epithelial cells exposing to calcium oxalate monohydrate[J]. SCIENTIFIC REPORTS,2018,8:8.
APA	Cao, Zhiqiang,Zhao, Jing,&Yang, Ke.(2018).Cu-bearing stainless steel reduces cytotoxicity and crystals adhesion after ureteral epithelial cells exposing to calcium oxalate monohydrate.SCIENTIFIC REPORTS,8,8.
MLA	Cao, Zhiqiang,et al."Cu-bearing stainless steel reduces cytotoxicity and crystals adhesion after ureteral epithelial cells exposing to calcium oxalate monohydrate".SCIENTIFIC REPORTS 8(2018):8.