IMR OpenIR
Cu-bearing stainless steel reduces cytotoxicity and crystals adhesion after ureteral epithelial cells exposing to calcium oxalate monohydrate
Cao, Zhiqiang1; Zhao, Jing2; Yang, Ke2
Corresponding AuthorCao, Zhiqiang(13309888392@163.com) ; Yang, Ke(kyang@imr.ac.cn)
2018-09-20
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume8Pages:8
AbstractCalcium oxalate monohydrate (COM), which is the main component of encrustation, may result in cell membrane injury. In addition, cellular damage is suggested to be the primary event attributing to COM crystal binding. To study the interaction between cells and crystals after incubating with a Cu-bearing stainless steel (316L-Cu SS), MTS and flow cytometric analyses were used to assess the cellular responses. The results confirmed that 316L-Cu SS could inhibit cytotoxicity and cellular apoptosis of ureteral epithelial cells (UECs) after COM treatment. Furthermore, molecular expressions of Cu/ Zn superoxide dismutase (CuZnSOD), which were evaluated by western blot analysis and real-time quantitative PCR (qPCR), indicated that 316L-Cu SS could inhibit the oxidative stress attributing to up-regulating of CuZnSOD. Moreover, the crystal adhesion cytokine CD44 was examined with western blot and qPCR, and the corresponding hyaluronic (HA) secreted into the medium was measured by enzyme-linked immunosorbent assay (ELISA). All results were confirmed that the expressions of cells cultured with 316L-Cu SS were down-regulated, demonstrating the inhibitory performance of 316L-Cu SS against crystal adhesion.
Funding OrganizationNational Natural Science Foundation of China ; General Financial Grant from China Postdoctoral Science Foundation
DOI10.1038/s41598-018-32388-0
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[51631009] ; National Natural Science Foundation of China[51371168] ; General Financial Grant from China Postdoctoral Science Foundation[2015M582885]
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000445079400015
PublisherNATURE PUBLISHING GROUP
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/129557
Collection中国科学院金属研究所
Corresponding AuthorCao, Zhiqiang; Yang, Ke
Affiliation1.Gen Hosp Shenyang Mil Reg, Shenyang 110840, Liaoning, Peoples R China
2.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Liaoning, Peoples R China
Recommended Citation
GB/T 7714
Cao, Zhiqiang,Zhao, Jing,Yang, Ke. Cu-bearing stainless steel reduces cytotoxicity and crystals adhesion after ureteral epithelial cells exposing to calcium oxalate monohydrate[J]. SCIENTIFIC REPORTS,2018,8:8.
APA Cao, Zhiqiang,Zhao, Jing,&Yang, Ke.(2018).Cu-bearing stainless steel reduces cytotoxicity and crystals adhesion after ureteral epithelial cells exposing to calcium oxalate monohydrate.SCIENTIFIC REPORTS,8,8.
MLA Cao, Zhiqiang,et al."Cu-bearing stainless steel reduces cytotoxicity and crystals adhesion after ureteral epithelial cells exposing to calcium oxalate monohydrate".SCIENTIFIC REPORTS 8(2018):8.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Cao, Zhiqiang]'s Articles
[Zhao, Jing]'s Articles
[Yang, Ke]'s Articles
Baidu academic
Similar articles in Baidu academic
[Cao, Zhiqiang]'s Articles
[Zhao, Jing]'s Articles
[Yang, Ke]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Cao, Zhiqiang]'s Articles
[Zhao, Jing]'s Articles
[Yang, Ke]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.