A Comparison of 1-and 3.2-MHz Low-Intensity Pulsed Ultrasound on Osteogenesis on Porous Titanium Alloy Scaffolds: An In Vitro and In Vivo Study

doi:10.1002/jum.14683

IMR OpenIR

	A Comparison of 1-and 3.2-MHz Low-Intensity Pulsed Ultrasound on Osteogenesis on Porous Titanium Alloy Scaffolds: An In Vitro and In Vivo Study
	Feng, Lifang 1; Liu, Xiaohan 1; Cao, Hongjuan 1; Qin, Limei 1; Hou, Wentao 2; Wu, Lin 1
通讯作者	Wu, Lin(wulin13@163.com)
	2019
发表期刊	JOURNAL OF ULTRASOUND IN MEDICINE
ISSN	0278-4297
卷号	38 期号:1 页码:191-202
摘要	Objectives Low-intensity pulsed ultrasound (LIPUS) combined with porous scaffolds can be used as a new therapy to treat bone defect repair. The aim of this study was to evaluate the effects of 1 and 3.2 MHz LIPUS on osteogenesis on porous Ti64 alloy scaffolds for both in vitro and in vivo studies. Methods Scaffolds were randomly divided into the high-frequency ultrasound group, low-frequency ultrasound group, and control group. Mouse pre-osteoblast cells were cultured with porous Ti-6Al-4V scaffolds in vitro to evaluate cell proliferation and differentiation. In addition, scaffolds were implanted into rabbit mandibular defects in vivo. The effects of LIPUS on bone regeneration were evaluated by observing the micro-computed tomography (micro-CT), toluidine blue staining, and von Kossa staining. Results The results revealed no significant difference in the cell counting kit-8 values between the ultrasound groups and control groups (P > .05). Compared with the control group, ultrasound promoted alkaline phosphatase activity and osteocalcin levels of the cells on the scaffolds (P < .05), but there was no significant difference between the two frequencies. In addition, histomorphologic analyses revealed that the volume and amount of new bone formation increased and that bone maturity improved in the ultrasound groups compared with the control group, but no significant difference was noted between the two frequencies. Conclusions Under the present experimental conditions, LIPUS promoted osteoblast differentiation and promoted bone maturity on porous Ti64 scaffolds. No significant differences were noted between the two frequencies.
关键词	bone formation frequency low-intensity pulsed ultrasound osteogenesis porous Ti64 scaffolds
资助者	National High Technology Research & Development Program (863 Program) of China ; Nature Science Foundation of Liaoning Province, China
DOI	10.1002/jum.14683
收录类别	SCI
语种	英语
资助项目	National High Technology Research & Development Program (863 Program) of China[2015AA033702] ; Nature Science Foundation of Liaoning Province, China[2014021008]
WOS研究方向	Acoustics ; Radiology, Nuclear Medicine & Medical Imaging
WOS类目	Acoustics ; Radiology, Nuclear Medicine & Medical Imaging
WOS记录号	WOS:000453869000022
出版者	WILEY
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/130900
专题	中国科学院金属研究所
通讯作者	Wu, Lin
作者单位	1.China Med Univ, Sch Stomatol, Dept Prosthodont, 117 Nanjing North St, Shenyang, Liaoning, Peoples R China 2.Chinese Acad Sci, Inst Met Res, Shenyang Natl Lab Mat Sci, Shenyang, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Feng, Lifang,Liu, Xiaohan,Cao, Hongjuan,et al. A Comparison of 1-and 3.2-MHz Low-Intensity Pulsed Ultrasound on Osteogenesis on Porous Titanium Alloy Scaffolds: An In Vitro and In Vivo Study[J]. JOURNAL OF ULTRASOUND IN MEDICINE,2019,38(1):191-202.
APA	Feng, Lifang,Liu, Xiaohan,Cao, Hongjuan,Qin, Limei,Hou, Wentao,&Wu, Lin.(2019).A Comparison of 1-and 3.2-MHz Low-Intensity Pulsed Ultrasound on Osteogenesis on Porous Titanium Alloy Scaffolds: An In Vitro and In Vivo Study.JOURNAL OF ULTRASOUND IN MEDICINE,38(1),191-202.
MLA	Feng, Lifang,et al."A Comparison of 1-and 3.2-MHz Low-Intensity Pulsed Ultrasound on Osteogenesis on Porous Titanium Alloy Scaffolds: An In Vitro and In Vivo Study".JOURNAL OF ULTRASOUND IN MEDICINE 38.1(2019):191-202.